Influence of the polarity of the microenvironment of pyrene on the intensity of its solid-phase luminescence

Мельников, Г. В.; Губина, Т. И.; Dyachuk, O. A.

doi:10.1134/s0036024406070284

Cited by 4 publications

(8 citation statements)

References 23 publications

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“…From the data in Table 1, we obtain the following ratio between the dielectric constant of the microenvironment of the pyrene molecules (ε m ) and the polarity index (I 1 /I 3 ), which is consistent with what was obtained earlier in [8]: (2).…”

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confidence: 82%

Complexation of pyrene and anthracene with human blood plasma

et al. 2008

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We have studied the interaction between polycyclic aromatic hydrocarbons (pyrene and anthracene) with human serum albumin (HSA) and human blood plasma. We have shown that the increase in the fluorescence intensity and the decrease in the polarity index of pyrene on going from an aqueous solution to a pH 7.4 buffer solution of HSA suggests that polycyclic aromatic hydrocarbons are localized in the hydrophobic microphase of the proteins. The increase in the fluorescence intensity for anthracene and pyrene, and also the decrease in the polarity index of pyrene on going from HSA to blood plasma is connected with the fact that polycyclic aromatic hydrocarbons can bind both to plasma proteins and to plasma lipids. When sodium dodecyl sulfate (SDS) is added to the blood plasma in a concentration greater than the critical micelle concentration, we observe an increase in the fluorescence intensity and the polarity index of pyrene. We hypothesize that this is connected with localization of pyrene near the interface between the hydrophobic and hydrophilic phases of the protein-SDS system. We have established that SDS leads to a change in the structure of blood plasma proteins and promotes escape of polycyclic aromatic hydrocarbons from the protein globules.Key words: human blood plasma, human serum albumin, polycyclic aromatic hydrocarbons, sodium dodecyl sulfate, fluorescence.Introduction. Luminescent probes are widely used in studying structural changes in proteins, lipoproteins, and also in studying the properties of biological membranes [1,2]. The properties of nonpolar microregions in microheterogeneous media have been studied using luminescent probes: polycyclic aromatic hydrocarbons, which due to hydrophobic interactions tend to escape from the polar aqueous medium or macrophase of the solution into the nonpolar microphase, i.e., into the interior volume of the surfactant micelles, which is followed from the changes in the vibrational structure of the fluorescence spectra of the probes [3]. The use of the fluorescence of pyrene, which belongs to the class of polycyclic aromatic hydrocarbons, is well known [4-6] for determination of changes in the physicochemical properties of biological systems and model lipid membranes. Analysis of the changes in the vibrational structure of the fluorescence spectra of pyrene [3-5] also makes it possible to obtain information about the polarity of the microenvironment of the luminescent probe when its position changes in different microheterogeneous media. In [6], using data from analysis of the vibronic structure of the monomer luminescence spectrum of pyrene in a model membrane of phosphatidylcholine liposomes, it was hypothesized that the pyrene molecules can not only move laterally along the surface of the membrane, but also can undergo transmembrane motion. Thus the use of a method based on analysis of changes in the vibronic structure of the fluorescence spectra of pyrene molecules is promising for studying processes connected with a change in where polycyclic aromatic hydroca...

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confidence: 82%

Complexation of pyrene and anthracene with human blood plasma

et al. 2008

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“…In the spectrum in a wavelength range of 370-400 nm, there are five main vibronic bands (I 1 ~375 nm, I 2 ~380 nm, I 3 ~385 nm, I 4 ~390 nm, and I 5 ~395 nm), of which the following three are well resolved: I 1 , I 3 , and I 5 . Such spectra are typical of the solutions of PAHs, in particular, pyrene [25,26]. It was established that the vibronic structure of the fluorescence spec trum of pyrene is sensitive to changes in the polarity of analyzed solutions.…”

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confidence: 99%

“…In the SPL method, phosphors are immobilized onto different solid phase matrices: aluminum oxide, quartz glass [27], zeolites [28], fiberglass [29], polyurethane foams [30], nylon membranes [31], silver [32] or gold [33] nanoparticles, etc. Filter paper (a cellulose matrix) is most com monly used [25,26]. However, the efficiency of the preconcentration of hydrophobic PAHs on filter paper is low because of the hydrophilicity of this sorbent.…”

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“…For this purpose, we prepared CAMs, evaluated their surface and phys icochemical properties, and studied the applicability of the membranes to luminescence analysis. Because the solubilization of a phosphor in surfactant micelles is a factor responsible for an improvement in the ana lytical characteristics of the luminescence determina tion of substances, in particular, pyrene [25,26,35], we used the following organized media (the self asso ciated ensembles of diphilic surfactant molecules [35]) in the experiments: the aqueous micellar surfactant + pyrene solutions, which contained anionic, cationic, and nonionic surfactants.…”

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“…Their concentrations in drinking water, wastewater, and aquatic environ ments should be monitored because of the ability of different PAHs to accumulate in environmental mate rials [24]. Pyrene is a readily available compound from Recent studies showed that solid phase lumines cence (SPL) is the most promising method for the determination of PAHs and, in particular, pyrene in aqueous media [25][26][27][28][29][30][31][32][33]. It provides an opportunity to combine the sorption preconcentration of a substance on a solid phase matrix with its subsequent lumines cence determination directly in the sorbent phase without a stage of PAH desorption by an organic sol vent.…”

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Usage of cellulose acetate membranes for the sorption-luminescence determination of pyrene in aqueous media

et al. 2015

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The applicability of cellulose acetate membranes (CAMs) as a solid matrix for the luminescence determination of pyrene in aqueous micellar solutions is shown. The effect of the concentrations of various surfactants, namely, anionic sodium dodecyl sulfate (SDS), cationic cetyltrimethylammonium bromide (CTAB), and nonionic polyoxyethylene (10) mono 4 isooctyl phenyl ether (TX 100), on the fluorescence of pyrene in aqueous micellar solutions before and after sorption preconcentration and in an adsorbed state on a CAM has been studied. It has been found that the fluorescence intensity of pyrene on the solid phase matrix increases as a result of pyrene solubilization in surfactant hemimiceles formed on the sorbent surface. The highest degree of pyrene extraction on CAMs has been achieved in the presence of cationic CTAB micelles. The CAM has a negative surface potential (-31.5 ± 2.5 mV), which affects the hydrocarbon recovery. The degree of extractlion and the polarity index of a microenvironment of pyrene molecules in solutions decrease in the order CTAB → SDS → TX 100.

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