Influence of the pH on the itaconic acid production with Aspergillus terreus

Hevekerl, Antje; Kuenz, Anja; Vorlop, Klaus‐Dieter

doi:10.1007/s00253-014-6047-2

Cited by 105 publications

(90 citation statements)

References 14 publications

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“…There was no significant difference in the pattern of glucose utilization and IA production among these 3 strains and final IA concentration was between 87 and 91 g L −1 . Recently, Hevekerl et al [30] reported that when pH control to 3.0 was started after 2.1 days of cultivation of A. terreus DSM 23081 in a medium containing 180 g glucose L −1 , the IA concentration reached to 146 g L −1 with a productivity of 0.48 g L −1 h −1 and a yield of 0.81 mol mol −1 glucose which is an increase of 68% compared to the cultivation without pH control. After 2.1 days, the cultivation had entered the production phase and IA was already formed.…”

Section: Production Of Itaconic Acid By Aspergillus Terreusmentioning

confidence: 98%

Emerging biotechnologies for production of itaconic acid and its applications as a platform chemical

Saha¹

2017

Journal of Industrial Microbiology and Biotechnology

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Recently, itaconic acid (IA), an unsaturated C5-dicarboxylic acid, has attracted much attention as a biobased building block chemical. It is produced industrially (>80 g L) from glucose by fermentation with Aspergillus terreus. The titer is low compared with citric acid production (>200 g L). This review summarizes the latest progress on enhancing the yield and productivity of IA production. IA biosynthesis involves the decarboxylation of the TCA cycle intermediate cis-aconitate through the action of cis-aconitate decarboxylase (CAD) enzyme encoded by the CadA gene in A. terreus. A number of recombinant microorganisms have been developed in an effort to overproduce it. IA is used as a monomer for production of superabsorbent polymer, resins, plastics, paints, and synthetic fibers. Its applications as a platform chemical are highlighted. It has a strong potential to replace petroleum-based methylacrylic acid in industry which will create a huge market for IA.

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Section: Production Of Itaconic Acid By Aspergillus Terreusmentioning

confidence: 98%

Emerging biotechnologies for production of itaconic acid and its applications as a platform chemical

Saha¹

2017

Journal of Industrial Microbiology and Biotechnology

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“…Although the most significant progress in improving itaconic acid production is made by classical process optimization techniques until now (Hevekerl et al 2014), the construction of mutant strains by genetic engineering is also promising for improving the itaconic acid production. For example, overexpression of CAD in A. terreus LYT10 enhanced the itaconic acid production level by nearly 10 % in shake flasks, and the production stage was shortened by around 4 h at the pilot scale (35 m 3 fermentor) (Huang et al 2014b).…”

Section: Discussionmentioning

confidence: 99%

Identification of an itaconic acid degrading pathway in itaconic acid producing Aspergillus terreus

Chen

Huang

Zhong

et al. 2016

Appl Microbiol Biotechnol

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Itaconic acid, one of the most promising and flexible bio-based chemicals, is mainly produced by Aspergillus terreus. Previous studies to improve itaconic acid production in A. terreus through metabolic engineering were mainly focused on its biosynthesis pathway, while the itaconic aciddegrading pathway has largely been ignored. In this study, we used transcriptomic, proteomic, bioinformatic, and in vitro enzymatic analyses to identify three key enzymes, itaconyl-CoA transferase (IctA), itaconyl-CoA hydratase (IchA), and citramalyl-CoA lyase (CclA), that are involved in the catabolic pathway of itaconic acid in A. terreus. In the itaconic acid catabolic pathway in A. terreus, itaconic acid is first converted by IctA into itaconyl-CoA with succinyl-CoA as the CoA donor, and then itaconyl-CoA is hydrated into citramalyl-CoA by IchA. Finally, citramalyl-CoA is cleaved into acetyl-CoA and pyruvate by CclA. Moreover, IctA can also catalyze the reaction between citramalyl-CoA and succinate to generate succinyl-CoA and citramalate. These results, for the first time, identify the three key enzymes, IctA, IchA, and CclA, involved in the itaconic acid degrading pathway in itaconic acid producing A. terreus. The results will facilitate the improvement of itaconic acid production by metabolically engineering the catabolic pathway of itaconic acid in A. terreus.

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“…Since it has been reported that controlling pH is important for IA production (Hevekerl et al, 2014), we performed fed-batch fermentation in which a pH of 6.2, the pH optimum for CadA, was constantly maintained by the addition of ammonia solution. Although cell mass in the fed-batch fermentation steadily increased, reaching $60-fold that in flask culture, IA production was just twofold higher (Fig.…”

Section: Ia Production In M9 Supplemented With Glycerol and Citric Acidmentioning

confidence: 99%

Itaconic acid production from glycerol using Escherichia coli harboring a random synonymous codon‐substituted 5′‐coding region variant of the cadA gene

Jeon

Cheong

Han

et al. 2016

Biotech & Bioengineering

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Aspergillus terreus cadA, encoding cis-aconitate decarboxylase, is an essential gene for itaconic acid (IA) biosynthesis, but it is primarily expressed as insoluble aggregates in most industrial hosts. This has been a hurdle for the development of recombinant strategies for IA production. Here, we created a library of synonymous codon variants (scv) of the cadA gene containing synonymous codons in the first 10 codons (except ATG) and screened it in Escherichia coli. Among positive clones, E. coli scvCadA_No8 showed more than 95% of expressed CadA in the soluble fraction, and in production runs, produced threefold more IA than wild-type E. coli in Luria-Bertani broth supplemented with 0.5% glucose. In M9 minimal media containing 0.85 g/L citrate and 1% glycerol, E. coli scvCadA_No8 produced 985.6 ± 33.4 mg/L IA during a 72-h culture after induction with isopropyl β-D-1-thiogalactopyranoside. In a 2-L fed-batch fermentation consisting of two stages (growth and nitrogen limitation conditions), we obtained 7.2 g/L IA by using E. coli by introducing only the scv_cadA gene and optimizing culture conditions for IA production. These results could be combined with metabolic engineering and generate an E. coli strain as an industrial IA producer. Biotechnol. Bioeng. 2016;113: 1504-1510. © 2015 Wiley Periodicals, Inc.

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Influence of the pH on the itaconic acid production with Aspergillus terreus

Cited by 105 publications

References 14 publications

Emerging biotechnologies for production of itaconic acid and its applications as a platform chemical

Emerging biotechnologies for production of itaconic acid and its applications as a platform chemical

Identification of an itaconic acid degrading pathway in itaconic acid producing Aspergillus terreus

Itaconic acid production from glycerol using Escherichia coli harboring a random synonymous codon‐substituted 5′‐coding region variant of the cadA gene

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