Influence of the patch pipette glass on single acetylcholine channels recorded from Xenopus myocytes

Rojas, Legier V.; Zuazaga, C.

doi:10.1016/0304-3940(88)90312-6

Cited by 15 publications

(5 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has long been recognized by some researchers that leaching of divalent ions (Ca 2+ , Mg 2+ , Pb 2+ ) from some types of glass used for patch-clamp electrodes can affect ionic currents, and that chelating agents can minimize such effects ( Cota and Armstrong, 1988 ; Rojas and Zuazaga, 1988 ). We were unable to find any references addressing the problem of Ca leaching from other forms of labware, although anecdotal evidence suggests that at least some researchers are aware of the possibility.…”

Section: Discussionmentioning

confidence: 99%

New method for determining total calcium content in tissue applied to skeletal muscle with and without calsequestrin

Lamboley

Guena

Touré

et al. 2015

Journal of General Physiology

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

New method for determining total calcium content in tissue applied to skeletal muscle with and without calsequestrin

Lamboley

Guena

Touré

et al. 2015

Journal of General Physiology

View full text Add to dashboard Cite

show abstract

“…The experiments were done at 22-24°C. Recording pipettes were pulled from soft glass (Rojas and Zuazaga, 1988) using 1 mm tubes (Fisher Scientific, Fairlawn, NJ, 02-668-68) in two steps. They were fire polished to diameters of about 1 µm, giving a resistance after filling of 4-6 M⍀.…”

Section: Methodsmentioning

confidence: 99%

K+ channel density increases selectively in the endfoot of retinal glial cells during development ofRana catesbiana

Rojas

Orkand

1999

Glia

View full text Add to dashboard Cite

The radial glial cells that span the retina, described by Müller in 1851, have a remarkable distribution of ion channels in adult amphibia that mediate extracellular K+ spatial buffering. 94% of the total membrane conductance of these cells resides in inward rectifier K+ channels in the endfoot processes apposed to the vitreous humour. We now report that this regional specialization is found in Müller cells isolated from adult (>120 day old) bullfrogs but to a far less extent in those from 10–20 day old tadpoles (stages 34–36). Using the cell attached configuration of the patch‐clamp technique, we found, in agreement with previous studies in salamanders, that the endfoot of adult cells had 19.2 ± 2.4 (mean ± S.E., n = 81) channels/patch, whereas the soma had 1.81 ± 0.28 (n = 21) channels/patch. In the tadpole, the respective values were 4.29 ± 0.26 (n = 79) for the endfoot and 2.26 ± 0.24 (n = 27) for the soma. The slope conductance of the inward rectifier K+ channel in 115 mM K+, 19.2 ± 0.25 pS (n = 205), channel kinetics and the resting membrane potential (−69 ± 2.7 mV, n = 224) were similar at both the endfoot and soma of both adults and embryos. We conclude that during development, the K+ conductance of the Müller cell endfoot, but not of the soma, increases due to a selective clustering of inwardly rectifyiing K+ channels in that specific region of the cell membrane. The properties of the channels change little during the transformation from tadpole to adult bullfrog. GLIA 25:199–203, 1999. © 1999 Wiley‐Liss, Inc.

show abstract

“…The pipettes were constructed using soft glass to avoid the channel rundown encountered when hard glass is used in embryonic muscle cells (Rojas and Zuazaga, 1988; Rojas, Bonilla, Báez, and Lasalde, 2003). Pipettes were filled with saline solution.…”

Section: Methodsmentioning

confidence: 99%

Triiodothyronine (T3) action on aquatic locomotor behavior during metamorphosis of the bullfrog Rana catesbeiana

Fernández-Mongil¹,

Venza²,

Rivera³

et al. 2009

Int. J. Dev. Biol.

View full text Add to dashboard Cite

Thyroid hormones -particularly triiodothyronine, T 3 -play a critical role in the morphological transformations comprising metamorphosis in larval bullfrogs (Rana catesbeiana). Traditional staging criteria for anuran larvae incompletely distinguish physiological and behavioral changes during growth. We therefore first developed a new parameter to describe larval growth, the developmental index (DI), which is simply the ratio between the tail length of the larva and its head diameter. Using the DI we were able to identify two distinct populations classifying the larvae during growth along a continuous linear scale with a cutoff value of DI at 2.8. Classification based on the DI, used in this study, proved an effective complement to existing classifications based on developmental staging into pre-or pro-metamorphic stages. Exposure to T 3 in the water induced a rapid (beginning within 5 min) and significant decrease (~20-40%) in locomotor activity, measured as total distance traversed and velocity. The largest decrease occurred in more developed larvae (DI< 2.8). To determine correlated changes in the neuromuscular junctions during metamorphosis and apoptotic tail loss, miniature endplate currents from tail muscle were recorded during acute exposure to a hypertonic solution, which simulates an apoptotic volume decrease. Our results support a role for T 3 in regulating larval locomotor activity during development, and suggest an enhanced response to volume depletion at the neuromuscular junction of older larvae (DI<2.8) compared to younger animals (DI≥2.8). We discuss the significance of the possible role of an apoptotic volume decrease at the level of the neuromuscular junction.

show abstract

Influence of the patch pipette glass on single acetylcholine channels recorded from Xenopus myocytes

Cited by 15 publications

References 12 publications

New method for determining total calcium content in tissue applied to skeletal muscle with and without calsequestrin

New method for determining total calcium content in tissue applied to skeletal muscle with and without calsequestrin

K+ channel density increases selectively in the endfoot of retinal glial cells during development ofRana catesbiana

Triiodothyronine (T3) action on aquatic locomotor behavior during metamorphosis of the bullfrog Rana catesbeiana

Contact Info

Product

Resources

About