1988
DOI: 10.1016/0304-3940(88)90312-6
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Influence of the patch pipette glass on single acetylcholine channels recorded from Xenopus myocytes

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Cited by 15 publications
(5 citation statements)
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“…It has long been recognized by some researchers that leaching of divalent ions (Ca 2+ , Mg 2+ , Pb 2+ ) from some types of glass used for patch-clamp electrodes can affect ionic currents, and that chelating agents can minimize such effects ( Cota and Armstrong, 1988 ; Rojas and Zuazaga, 1988 ). We were unable to find any references addressing the problem of Ca leaching from other forms of labware, although anecdotal evidence suggests that at least some researchers are aware of the possibility.…”
Section: Discussionmentioning
confidence: 99%
“…It has long been recognized by some researchers that leaching of divalent ions (Ca 2+ , Mg 2+ , Pb 2+ ) from some types of glass used for patch-clamp electrodes can affect ionic currents, and that chelating agents can minimize such effects ( Cota and Armstrong, 1988 ; Rojas and Zuazaga, 1988 ). We were unable to find any references addressing the problem of Ca leaching from other forms of labware, although anecdotal evidence suggests that at least some researchers are aware of the possibility.…”
Section: Discussionmentioning
confidence: 99%
“…The experiments were done at 22-24°C. Recording pipettes were pulled from soft glass (Rojas and Zuazaga, 1988) using 1 mm tubes (Fisher Scientific, Fairlawn, NJ, 02-668-68) in two steps. They were fire polished to diameters of about 1 µm, giving a resistance after filling of 4-6 M⍀.…”
Section: Methodsmentioning
confidence: 99%
“…The pipettes were constructed using soft glass to avoid the channel rundown encountered when hard glass is used in embryonic muscle cells (Rojas and Zuazaga, 1988; Rojas, Bonilla, Báez, and Lasalde, 2003). Pipettes were filled with saline solution.…”
Section: Methodsmentioning
confidence: 99%