Influence of the nutritional conditions on haloalcohol dehalogenase HheC production by recombinant Escherichia coli P84A/MC1061

Xiao, Li; Li, Jianhua; Tan, Ya-Li; Dong-rui, Pan; Li, Zhihong; Yu, Xuefeng; Yao, Juan

doi:10.1007/s13213-012-0582-9

Ann Microbiol

2013

DOI: 10.1007/s13213-012-0582-9

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Influence of the nutritional conditions on haloalcohol dehalogenase HheC production by recombinant Escherichia coli P84A/MC1061

Li Xiao

Jianhua Li²,

Ya-Li Tan

et al.

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Effect of Zn²⁺ on halohydrin dehalogenase expression and accumulation through multi-parameter correlation research with Escherichia coli P84A/MC1061

Luo

Dong-rui

et al. 2017

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2C was confirmed as the significant factor, and its optimal concentration for HheC expression was 3.87 mg/L through fermentation experiments in shaking flasks. Second, based on experimental results from the different strategies, it was found that PA, nutrient consumption rate (NCR) and specific growth rate (m) for E. coli P84A/MC1061 were promoted in the log phase (4-8 h) under appropriate Zn 2C concentrations in the lag phase and late log phase. Furthermore cell biomass was also increased to a higher level and the maximum HheC activity (i.e. HheC max ) was increased by 9.80%, and the time to reach HheC max was reduced from 16 to 12 hours. Furthermore, appropriate supplementation of Zn 2C caused higher m for E. coli P84A/MC1061, which resulted in more rapid accumulation of increased acetic acid concentrations, leading to higher acetic acid consumption avoiding any negative effects on producing HheC because of carbon source being exhausted prematurely and acetic acid being consumed rapidly.

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Effect of Zn²⁺ on halohydrin dehalogenase expression and accumulation through multi-parameter correlation research with Escherichia coli P84A/MC1061

Luo

Dong-rui

et al. 2017

Bioengineered

Self Cite

View full text Add to dashboard Cite

show abstract

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Influence of the nutritional conditions on haloalcohol dehalogenase HheC production by recombinant Escherichia coli P84A/MC1061

Cited by 1 publication

References 28 publications

Effect of Zn²⁺ on halohydrin dehalogenase expression and accumulation through multi-parameter correlation research with Escherichia coli P84A/MC1061

Effect of Zn²⁺ on halohydrin dehalogenase expression and accumulation through multi-parameter correlation research with Escherichia coli P84A/MC1061

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Influence of the nutritional conditions on haloalcohol dehalogenase HheC production by recombinant Escherichia coli P84A/MC1061

Cited by 1 publication

References 28 publications

Effect of Zn2+ on halohydrin dehalogenase expression and accumulation through multi-parameter correlation research with Escherichia coli P84A/MC1061

Effect of Zn2+ on halohydrin dehalogenase expression and accumulation through multi-parameter correlation research with Escherichia coli P84A/MC1061

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Effect of Zn²⁺ on halohydrin dehalogenase expression and accumulation through multi-parameter correlation research with Escherichia coli P84A/MC1061

Effect of Zn²⁺ on halohydrin dehalogenase expression and accumulation through multi-parameter correlation research with Escherichia coli P84A/MC1061