Influence of the microstructure on the shell strength of eggs laid by hens of different ages

Rodríguez‐Navarro, Alejandro B.; Kälin, O.; Nys, Yves; García‐Ruiz, Juan Manuel

doi:10.1080/00071660120103675

Cited by 177 publications

(125 citation statements)

References 22 publications

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“…Increased egg weight with increasing hen age has been reported previously by many authors (Guesdon and Faure 2004;Roberts and Ball 2006;Tumova and Ledvinka 2009). The decrease in shell breaking strength as hens age, found in this study, has also been reported previously (Rodriguez-Navarro et al 2002;Roberts and Ball 2006). Shell deformation to breaking point is an indicator of the degree of elasticity of the eggshell.…”

Section: Discussionsupporting

confidence: 72%

Egg quality and age of laying hens: implications for product safety

Roberts¹,

Chousalkar²,

Samiullah³

2013

Anim. Prod. Sci.

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Abstract. Eggs were collected from commercial caged layer flocks in early, mid, late and very late lay. Eggs were candled and scored for translucency. Cuticle cover was estimated using MST Cuticle Stain and a Konica Minolta hand-held spectrophotometer. Traditional measures of egg quality were determined using specialised equipment. Shell ultrastructural features were scored following plasma ashing of shell samples and viewing under a benchtop scanning electron microscope. Translucency score was significantly higher in late lay than for all other age groups. Shell quality declined with increasing flock age. However, the extent of cuticle cover on the egg shell was not significantly different among flock age groups. The incidence of shell ultrastructural features associated with good quality shells was lower for older flocks and incidence of ultrastructural features associated with poorer quality shells was higher for older flocks. Translucency score had a low correlation with the ultrastructural features of the mammillary layer.

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Section: Discussionsupporting

confidence: 72%

Egg quality and age of laying hens: implications for product safety

Roberts¹,

Chousalkar²,

Samiullah³

2013

Anim. Prod. Sci.

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“…Smaller crystals in the eggshell are more compact and increase the strength of the eggshell. Structural differences can be associated with decreasing eggshell strength with age; however, the eggshell thickness was not influenced, which corresponds with Rodriguez-Navarro et al (2002). The authors reported a weaker correlation between eggshell strength and thickness or weight in young hens than older ones.…”

Section: Discussionmentioning

confidence: 85%

Effect of housing system and age of laying hens on eggshell quality, microbial contamination, and penetration of microorganisms into eggs

Vlčková¹,

Tůmová²,

Ketta³

et al. 2018

Czech J. Anim. Sci.

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Vlčková J., Tůmová E., Ketta M., Englmaierová M., Chodová D. (2018): Effect of housing system and age of laying hens on eggshell quality, microbial contamination, and penetration of microorganisms into eggs. Czech J. Anim. Sci., 63, 51-60.Hens of the laying hybrid ISA Brown were used in the study with the objective to evaluate eggshell quality, microbial contamination of eggshells, and penetration of microorganisms into the egg content in different housing systems (enriched cage: 60 hens, 10 hens per cage, 750 cm 2 per hen vs free range: 60 hens, 9 hens per m 2 ) and at different hen ages (26 vs 51 weeks) during storage time (0, 2, 7, 14, and 21 days). A significant interaction between the housing system and age was observed in egg weight and most of eggshell quality measurements. However, microbial contamination and penetration were affected mostly by the housing system and storage time. The numbers of Escherichia coli (P < 0.001, 4.51 vs 2.75 log cfu/eggshell) and Enterococcus (P < 0.001, 2.56 vs 1.11 log cfu/eggshell), and the total number of microorganisms (P < 0.001, 5.04 vs. 3.65 log cfu/eggshell) were higher in free range eggs compared to enriched cage eggs, respectively. The counts of Escherichia coli (P < 0.001, 4.23 vs 2.91 log cfu/eggshell) and Enterococcus (P < 0.001, 2.31 vs 1.27 log cfu/eggshell) decreased with storage time. A positive correlation between the total number of pores and penetration of Escherichia coli in both housing systems was observed in the albumen. It can be concluded that the housing system and age of laying hens significantly affected eggshell quality. Microbial contamination presumably affects the penetration of microorganisms. The correlation between the number of pores and penetration is assumed to be affected by the microbial species.

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“…These imaging modalities revealed that occluded osteopontin is present on the surface of cleaved calcite along the {104} crystallographic faces. The elongated calcite crystals in the palisades region tend to be preferentially orientated with their (001) planes parallel (c-axis perpendicular) to the shell surface, which would orient the {104} plane at 44j tangential to the surface (Silyn-Roberts and Sharp 1986;Rodriguez-Navarro et al 2002). The {104} calcite face is the natural cleavage plane, and specific osteopontin binding to this growing crystal face during mineralization could modify the resistance of the shell to fracture along this plane.…”

Section: Discussionmentioning

confidence: 99%

Colloidal-gold Immunocytochemical Localization of Osteopontin in Avian Eggshell Gland and Eggshell

Hincke

Chien

Gerstenfeld

et al. 2008

J Histochem Cytochem.

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S U M M A R Y During mineralization of the avian eggshell, there is a sequential and orderly deposition of both matrix and mineral phases. Therefore, the eggshell is an excellent model for studying matrix-mineral relationships and the regulation of mineralization. Osteopontin, as an inhibitor of crystal growth, potently influences the formation of calcium phosphate and calcium carbonate biominerals. The purpose of this study was to characterize matrix-mineral relationships, specifically for osteopontin, in the avian eggshell using high-resolution transmission (TEM) and scanning (SEM) electron microscopy to gain insight into how calcite crystal growth is structured and compartmentalized during eggshell mineralization. Osteopontin was localized at the ultrastructural level by colloidal-gold immunocytochemistry. In EDTAdecalcified eggshell, an extensive matrix network was observed by TEM and SEM throughout all regions and included interconnected fibrous sheets, irregularly shaped aggregates, vesicular structures, protein films, and isolated protein fibers. Osteopontin was associated with protein sheets in the highly mineralized palisades region; some of these features defined boundaries that compartmentalized different eggshell structural units. In fractured and undecalcified eggshell, osteopontin was immunolocalized on the {104} crystallographic faces of calcite-its natural cleavage plane. The specific occlusion of osteopontin into calcite during mineralization may influence eggshell structure to modify its fracture resistance. (J Histochem Cytochem 56:467-476, 2008)

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Influence of the microstructure on the shell strength of eggs laid by hens of different ages

Cited by 177 publications

References 22 publications

Egg quality and age of laying hens: implications for product safety

Egg quality and age of laying hens: implications for product safety

Effect of housing system and age of laying hens on eggshell quality, microbial contamination, and penetration of microorganisms into eggs

Colloidal-gold Immunocytochemical Localization of Osteopontin in Avian Eggshell Gland and Eggshell

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