Influence of the disaccharide trehalose on the oxidizing side of photosystem II

Mamedov, Mahir D.; Nosikova, Ekaterina S.; Vitukhnovskaya, Liya A.; Zaspa, A. A.; Semenov, Alexey Yu.

doi:10.1007/s11099-017-0750-z

Cited by 8 publications

(1 citation statement)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was shown that trehalose protects PSII during thawing [69,70] and prevents the aggregation and inactivation of PSII membrane fragments during long-term storage [71], although it does not exhibit a protective effect during the thermal inactivation of isolated PSII D1/D2/cyt b 559 reaction center complexes of PSII [62]. It has been demonstrated that the addition of trehalose stabilizes electron transport in oxygen-evolving PSII preparations [72,73]. In addition to the protective properties, trehalose acts on electronic transport in bacterial type 2 reaction centers [74] and apo-WOC-PSII [75].…”

Section: Introductionmentioning

confidence: 99%

Effect of Osmolytes on Photoassembly of Functionally Active Mn4CaO5 Cluster in Mn-Depleted Photosystem II Preparations Isolated from Spinach Leaves

Yanykin,

Kazantseva,

Khorobrykh

2023

Horticulturae

View full text Add to dashboard Cite

The effect of osmolytes (trehalose, sucrose, betaine, D-glucose and hydroxyectoine) on the photoassembly of the functionally active inorganic core of the water-oxidizing complex (Mn4CaO5 cluster) in Mn-depleted PSII preparations (apo-WOC-PSII) was investigated. It was revealed that the efficiency of the photoassembly of the Mn4CaO5 cluster was considerably (three times) increased in the presence of 1 M disaccharides (trehalose or sucrose) in contrast to other osmolytes. It was found that the osmolytes (especially trehalose or sucrose) improved the redox interaction of exogenous Mn2+ with apo-WOC-PSII, enhanced the protective effect of Mn2+ against the photoinhibition of apo-WOC-PSII, protected CaCl2-treated PSII preparations against thermoinactivation, and stabilized the water-oxidizing complex and electron transport from QA to QB in native PSII preparations during heat treatment. It is suggested that the ability of osmolytes to enhance the efficiency of the photoassembly of a Mn4CaO5 cluster depends on their effect on the following key processes: the redox interaction of Mn2+ with apo-WOC-PSII; the stability of apo-WOC-PSII to photoinhibition during the photoactivation procedure; and the stabilization of both the newly assembled functionally active Mn4CaO5 cluster and the electron transfer from QA to QB.

show abstract