Influence of the digestive enzymes trypsin and pepsin in vitro on the progression of erosion in dentine

Schlueter, Nadine; Hardt, Martin; Klimek, Joachim; Ganß, Carolina

doi:10.1016/j.archoralbio.2010.02.003

Cited by 53 publications

(59 citation statements)

References 31 publications

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“…These surfaces might approximate the clinical structure of active erosive lesions under severe dental erosion (e.g. caused by eating disorders with chronic vomiting) and the potential continuous removal of organic matrix by the multitude of salivary [26] or gastric proteolytic enzymes [27]. In all groups with continuous removal of demineralised organic matrix bond strength values were higher than in the groups without removal.…”

Section: Discussionmentioning

confidence: 98%

The effect of a tin-containing fluoride mouth rinse on the bond between resin composite and erosively demineralised dentin

et al. 2012

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The effect of a tin-containing fluoride mouth rinse on the bond between resin composite and erosively demineralised dentin Abstract Objectives: To evaluate the effect of a tin-containing fluoride (Sn/F) mouth rinse on microtensile bond strength (µTBS) between resin composite and erosively demineralised dentin. Materials and Methods:Dentin of 120 human molars was erosively demineralised using a 10-day cyclic de-and remineralisation model. For 40 molars the model comprised erosive demineralisation only, for another 40 the model included treatment with NaF solution, and for yet another 40 the model included treatment with a Sn/F mouth rinse. In half of these molars (n=20) the demineralised organic matrix was continuously removed by collagenase. SiC paper ground, non-erosively demineralised molars served as control (n=20). Subsequently, µTBS of Clearfil SE/Filtek Z250 to the dentin was measured and failure mode determined.Additionally, surfaces were evaluated by SEM and EDX.Results: Compared to the non-erosively demineralised control, erosive demineralisation resulted in significantly lower µTBS regardless of the removal of demineralised organic matrix. Treatment with NaF increased µTBS, but the level of µTBS obtained by the non-erosively demineralised control was only reached when the demineralised organic matrix had been removed. The Sn/F mouth rinse led to significantly higher µTBS than did the non-erosively demineralised control, both with or without removal of demineralised organic matrix. The Sn/F mouth rinse yielded higher µTBS than did the NaF solution.Conclusions: Treatment of erosively demineralised dentin with a NaF solution or a Sn/F mouth rinse increased the bond strength of resin composite.Clinical Relevance: Bond strength of resin composite to eroded dentin was not negatively influenced by treatment with a tin-containing fluoride mouth rinse.

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Section: Discussionmentioning

confidence: 98%

The effect of a tin-containing fluoride mouth rinse on the bond between resin composite and erosively demineralised dentin

et al. 2012

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“…[31][32][33][34] In a recent ex vivo study the additive erosive effects of pepsin and trypsin on teeth were shown. 35 Further elaboration of the interaction of the erosive effects of acid with other salivary factors is needed. An additional factor contributing to the lack of association between the reflux characteristics measured by pH-MII and the degree of dental erosions is the difficulty of correlating acute measures of 24-h duration with effects of longstanding reflux.…”

Section: Discussionmentioning

confidence: 99%

Gastro‐oesophageal reflux is common in oligosymptomatic patients with dental erosion: A pH‐impedance and endoscopic study

Wilder‐Smith

Materna²,

Martig

et al. 2015

UEG Journal

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Background: Dental erosion is a complication of gastro-oesophageal reflux disease (GORD) according to the Montreal consensus statement. However, GORD has not been comprehensively characterized in patients with dental erosions and pH-impedance measures have not been reported. Objectives: Characterize GORD in patients with dental erosions using 24-h multichannel intraluminal pH-impedance measurements (pH-MII) and endoscopy. Methods: This single-centre study investigated reflux in successive patients presenting to dentists with dental erosion using pH-MII and endoscopy. Results: Of the 374 patients, 298 (80%) reported GORD symptoms <2 per week, 72 (19%) had oesophagitis and 59 (16%) had a hiatal hernia. In the 349 with pH-MII the mean percentage time with a pH <4 (95% CI) was 11.0 (9.3-12.7), and 34.4% (31.9-36.9) for a pH <5.5, a critical threshold for dental tissue. The mean numbers of total, acidic and weakly acidic reflux episodes were 71 (63-79), 43 (38-49) and 31 (26-35), respectively. Of the reflux episodes, 19% (17-21) reached the proximal oesophagus. In 241 (69%) patients reflux was abnormal using published normal values for acid exposure time and reflux episodes. No significant associations between the severity of dental erosions and any reflux variables were found. The presence of GORD symptoms and of oesophagitis or a hiatal hernia was associated with greater reflux, but not with increased dental erosion scores. Conclusions: Significant oligosymptomatic gastro-oesophageal reflux occurs in the majority of patients with dental erosion. The degree of dental erosion did not correlate with any of the accepted quantitative reflux indicators. Definition of clinically relevant reflux parameters by pH-MII for dental erosion and of treatment guidelines are outstanding. Gastroenterologists and dentists need to be aware of the widely prevalent association between dental erosion and atypical GORD.

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“…It is well known that beside hydrochloric acid [34], the gastric juice also contains various enzymes, including the proteolytic enzyme pepsin [35]. The results of studies [36,37] using pepsin admixture to hydrochloric acid for erosion simulation are inconclusive. Therefore, in the present study, the erosive attack was performed with pure hydrochloric acid, as performed in numerous other studies [12,14,19,24,38,39].…”

Section: Discussionmentioning

confidence: 99%

Durability of the anti-erosive effect of surfaces sealants under erosive abrasive conditions

Wegehaupt

Tauböck

Attin

2013

Acta Odontologica Scandinavica

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OBJECTIVE: To test the durability of sealants applied for prevention of erosive dentine mineral loss under erosive/abrasive conditions. METHODS: Forty-eight bovine dentine samples doped with (32)P were randomly allocated to four groups (1-4). All samples performed a de-and remineralizations precycling (6 × 1 min erosion in HCl: pH 3.0, mean time and overnight immersion in artificial saliva) for 1 day. Sealing was done as follows; (1) unsealed, (2) Seal Protect, (3) K-0184 (experimental sealer) and (4) OptiBond FL. After sealing, samples were immersed in HCl for 3 h (baseline measurement). Then, the following erosive/abrasive and remineralisations cycling was performed for 8 days: 3 h/day erosion with HCl, 600 brushing strokes/day and storage in artificial saliva for the rest of the day. Sealer permeability was evaluated by assignation of (32)P in the acid used for the erosive attacks. RESULTS: At baseline, the significantly highest dentine loss was observed for the unsealed control group, while the mineral loss was not statistically significantly different between the sealed groups 2 and 3. At all days of the erosive/abrasive and remineralizations cycling and cumulatively the significantly highest mineral loss was observed for group 1, while the significantly lowest mineral loss was observed for the samples sealed with Seal Protect (group 2) and K-0184 (group 3). In all groups, no significant increase in the (32)P release was observed. CONCLUSION: Surface sealants are able to reduce the erosive dentine mineral loss and maintain this erosion-preventing efficacy over the whole duration (simulating 8 month in-vivo) of the erosive/abrasive cycling. Abstract:Objective: To test the durability of sealants applied for prevention of erosive dentine mineral loss under erosive/abrasive conditions.Methods: 48 bovine dentine samples doped with 32 P were randomly allocated to four groups(1-4). All samples performed a de-and remineralisations precycling (6 x 1 min erosion in HCl: pH 3.0, mean time and over night immersion in artificial saliva) for one day. Sealing was done as follows; 1: unsealed, 2: Seal&Protect, 3: K-0184 (experimental sealer) and 4:OptiBond FL. After sealing, samples were immersed in HCl for 3 h (baseline measurement).Then, the following erosive/abrasive and remineralisations cycling was performed for 8 days:3 h/day erosion with HCl, 600 brushing strokes/day and storage in artificial saliva for the rest of the day. Sealer permeability was evaluated by assignation of 32 P in the acid used for the erosive attacks.Results: At baseline, the significantly highest dentine loss was observed for the unsealed control group, while the mineral loss was not statistically significantly different between the sealed groups 2-3.At all days of the erosive/abrasive and remineralisations cycling, and cumulative the significantly highest mineral loss was observed for group 1, while the significantly lowest mineral loss was observed for the samples sealed with Seal&Protect (group 2) and K-0184 (group 3). In all group...

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Influence of the digestive enzymes trypsin and pepsin in vitro on the progression of erosion in dentine

Cited by 53 publications

References 31 publications

The effect of a tin-containing fluoride mouth rinse on the bond between resin composite and erosively demineralised dentin

The effect of a tin-containing fluoride mouth rinse on the bond between resin composite and erosively demineralised dentin

Gastro‐oesophageal reflux is common in oligosymptomatic patients with dental erosion: A pH‐impedance and endoscopic study

Durability of the anti-erosive effect of surfaces sealants under erosive abrasive conditions

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