Influence of the deprivation of a single amino acid on cellular proliferation and survival in rat 3Y1 fibroblasts and their derivatives transformed by a wide variety of agents

Abstract

We compared proliferation and survival of various syngeneic transformed cell lines under conditions of depletion of 15 amino acids in Dulbecco-Eagle's medium. We used a normal fibroblast line 3Y1 and 22 transformed sublines of 3Y1 which had been induced by one of seven transforming agents--simian virus 40, mouse polyomavirus, adenovirus type 12, E1A gene of adenovirus type 12, cDNA of Harvey murine sarcoma virus, Rous sarcoma virus, or N-methyl-N'-nitro-N-nitrosoguanidine. Unlike other untransformed cells examined (mouse BALB/c-3T3 line, mouse NIH-3T3 line, and primary Fischer rat embryo fibroblasts), 3Y1 ceased to proliferate and accumulated in a viable state with a G1-phase DNA content under 14 singular deprivations of amino acid. None of the transformed 3Y1 lines completely arrested in the G1 phase of the cell cycle and each showed different levels of survival, depending on each transforming agent. As for transformed 3Y1 cells induced by a given virus or a given transforming gene, any one of the three sublines shared the same trend with respect to proliferation and survival. Transformed derivatives induced by N-methyl-N'-nitro-N-nitrosoguanidine showed almost the same trend in proliferation, but the patterns of survival were not uniform. Our observations suggest that the unique responses of 3Y1 to amino acid depletion are differently modified by different transforming agents.

Single amino acid (arginine) deprivation: rapid and selective death of cultured transformed and malignant cells

“…In a similar matched pair comparison, 3T3 cells were found to die relatively quickly in -Arg conditions, being not as fast as its virally transformed subline, 3T3-SV40. NIH 3T3 and BIBR 3T3 were equally sensitive (Table 1, see also Tanaka et al, 1988) to the former cell type. Thus 3T3 cells behave in the manner of transformed lines, unlike IBR3 fibroblasts (see Discussion).…”

Selective Expression and Functional Characteristics of Three Mammalian Hyaluronan Synthases in Oncogenic Malignant Transformation

“…The rat 3Y1 fibroblastic cell line has been utilized as a model system to study transforming activities of oncogenes (32)(33)(34)(35)(36)(37). Transformed 3Y1 cells bearing adenovirus E1A fragment or simian virus 40 show slow rate of tumorigenesis despite of the increases in both saturation cell density and anchorage-independent growth (33,35).…”

Cell Adhesion Kinase β Forms a Complex with a New Member, Hic-5, of Proteins Localized at Focal Adhesions

“…Fluorescein isothiocyanate-conjugated anti-rabbit immunoglobulins and rhodamine-conjugated anti-mouse immunoglobulins were purchased from DAKO (Copenhagen, Denmark (31), and its parent line, 3Y1-B clone 1-6 (3Y1, RCB0488) (32), were obtained from Riken Cell Bank (Tsukuba, Japan). COS-7 (ATCC CRL 1651) and HEK 293 (CRL 1573) were obtained from the American Type Culture Collection (Rockville, MD).…”