Influence of the combination of last sense codon and stop codon on expression efficiency of green fluorescent protein gene in Escherichia coli when the expression vector pKK223-3

Abstract

In this paper, the influence of the combination of last sense codon and stop codon on expression efficiency was studied. By our study, for the last sense codon CCG, the RFI of GFP(CCG) was 2.1 fold when the stop codon was UAA, but in comparison, the RFI was 1.1 fold when the stop codon was changed from UAA to UAG. For last sense codon TAG, the RFI of GFP(TAG) with the stop codon UAG was stronger than that with the stop codons UAA and UGA.