Influence of the Coat Color on the Trace Elemental Status Measured by Particle-Induced X-ray Emission in Horse Hair

Asano, Kazushi; Suzuki, Kazuyuki; Chiba, Momoko; Sera, K.; Matsumoto, Tsutomu; Asano, Ryuji; Sakai, Takeo

doi:10.1385/bter:103:2:169

Cited by 20 publications

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References 23 publications

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“…In a previous study, we demonstrated that the values obtained are not dependent on the horse's age, breed, and sex, and that they may be used as reference values in the assessment of diseases and nutritional status in horses [2]. However, the influence of hair color on elemental status has already been demonstrated in the horse [1,5]. In order to avoid the influence of hair color, therefore, all mane hair samples were obtained from bay horses whose mane hair color was black.…”

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confidence: 85%

“…A total of 10 hairs were collected as hair specimens from the nape area nearest to the skin of each horse. The hair specimens were further cut to even lengths of about 6 cm and washed with acetone a few times [1][2][3]. Only 10 hairs were attached per holder and directly irradiated with proton beams.…”

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confidence: 99%

“…Dunnett and Lees [7] demonstrated that mane hair growth was relatively constant over a 12-month period. In addition, hair must be more than 6 cm in length for PIXE measurement [1]. Therefore, it seemed that mane hair was the most suitable specimen for determination of hair element status.…”

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Relationship between Trace Elements Status in Mane Hair and Atrial Fibrillation in Horse

Asano

Suzuki

Chiba

et al. 2006

The Journal of Veterinary Medical Science

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ABSTRACT. To investigate the relationships between trace elements concentrations in hair and atrial fibrillation (AF) in horses, concentrations of nineteen trace elements were detected in hair using the particle induced X-ray emission (PIXE) method. The horses were assigned to either control (n=22, no abnormalities) or AF groups (n=5) based on electrocardiograph findings. The mean concentrations of Ca and Zn in the hair of the AF group were significantly higher than those in the control group. The Zn/Cu ratio of the hair in the AF group (29.8 ± 5.5) was significantly higher than that in the control group (23.4 ± 2.2, P<0.05). The results of the present study suggest that there is a relationship between elevated concentrations of Ca and Zn in hair and AF. KEY WORDS: atrial fibrillation, equine, trace element.J. Vet. Med. Sci. 68 (7): [769][770][771] 2006 Atrial fibrillation (AF) is the most common clinically relevant cardiac arrhythmia in horses [10]. Using electrocardiography, AF is identified by the lack of definitive P waves, presence of deflections of variable frequency and amplitude in the baseline (f waves) attributable to chaotic electrical activity in the atria, and an irregular ventricular rate. Older horses with AF often have signs of congestive heart failure, prominent murmurs, and a rapid resting heart rate [6,9].Many reports have discussed the relevance of Zn in arrhythmias in human [11], guinea pigs [4,8] and rats [14]. We previously described that horses with first and second degrees atrioventricular (AV) blocks have a large amount of Zn in hair compared to those without arrhythmia [3]. Those results suggested that evaluation of the concentration of Zn in hair might be used to predict the susceptibility of horses to heart disease much earlier than the appearance of symptoms and development of heart disease. However, correlations between arrhythmia and element status in hair are not yet clear, except in the case of AV block.Therefore, investigation of the relationship between AF and trace element status is important for equine health care. The aim of this study was to investigate the relationships between mean concentrations of those elements and AF in the mane hair of horses.Experiments were performed on 27 castrated and bay horses, 13.2 ± 4.2 year-old. The horses used in this study were kept under the same management conditions for at least three months in the same riding club (Yokohama, Kanagawa) and were deemed healthy on the basis of physical examinations. Electrocardiograms (ECG) of the resting horses were taken by a portable cardiac monitor (PAM Cardiac Monitor VM-8000, West Lagayette, IN, U.S.A.) and recorded for one minute in a stable. The horses were assigned to either the control (n=22, no abnormalities) or AF groups (n=5, subclinically established atrial fibrillation) based on ECG findings. In a previous study, we demonstrated that the values obtained are not dependent on the horse's age, breed, and sex, and that they may be used as reference values in the assessment of diseases and n...

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Relationship between Trace Elements Status in Mane Hair and Atrial Fibrillation in Horse

Asano

Suzuki

Chiba

et al. 2006

The Journal of Veterinary Medical Science

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“…Für Haaranalysen bei Pferden wurden von in Ostdeutschland, Polen und Ungarn sowie Asano et al ( , 2005aAsano et al ( , 2005c in Japan MH, von Cape und Hinz (1982) in Ithaka, USA, sowie Wichert et al (2002) Bei diagnostischen Fragen reflektieren Blutanalysen für Se, Cu und Zn aktuelle Mangelsituationen gut, die Haaranalyse informiert über mögliche Versorgungsstörungen in längeren Zeiträumen (Anke und Risch 1979, Anke und Müller 2011, Anke et al 2002, Frank 2001, Murase et al 2012, Coenen 2016 …”

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“…Alters-, Rasse-und Geschlechtsunterschiede bestanden nicht. Bei weiteren 56 Pferden prüften Asano et al (2005c) den Einfluss der Pigmentierungen weiß, braun sowie schwarz. Der Spurenelementgehalt in weißem (w) Haar unterschied sich z. T. von dem in braunem (b) und schwarzem (s) MH,z.…”

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Selenium, copper and zinc contents in brown, black and white mane, coat and tail hairs in horses of two locations

Ratjen¹,

Anke²,

Fürll³

2017

PHK

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Schlüsselwörter: Spurenelemente, Versorgungsstatus, Pferdehaare, Lebensräume, Haararten, Nachweis, Fütterung Selenium, copper and zinc contents in brown, black and white mane, coat and tail hairs in horses of two locationsThe hair analysis is a method also to control the supply of trace elements. In horses, there is this little systematic investigation. The aim of the study was in German riding horses to analyze possible effects of hair localization, pigmentation and gender as well as of different habitat for Se, Cu and Zn hair, to describe reference values for mane, coat and tail hair and to formulate a diagnostic procedure. We investigated in September black, brown and white mane, coat and tail hair of 73 healthy German riding horses, including 33 horses (13 geldings, 20 mares) in Bavaria and 40 (22 geldings, 18 mares) in Lower Saxony. They were fed only with oats and hay from the respective regions without additional mineral mixtures and were daily grazing. 82Se, 63Cu and 68Zn in the hair were measured by the ICP-MS Perkin Elmer ELAN 6000. Mane and tail hair contained significantly more Se and Cu as a coat (p < 0.05). The mane saved more Zn than coat and tail hair (p < 0.05). Se was most in black hair (p < 0.05). The pigmentation did not affect the Zn and Cu content of horse hair. In Se, the 10th and 90th percentiles overlapped in brown and black hair, and lay between 450 -1700 µg Se/kg DM; in white hair, they ranged from 325 -1600 µg/kg DM. In Cu fluctuated the 10th to 90th percentile in mane and tail hair between 4.6 to 7.9 mg/kg DM for all pigmentations. White hair stored Zn significantly more than the black (p < 0.05); percentiles ranged from 127 to 180 mg Zn/kg DM, in brown and black between 119 -178 mg Zn/kg MD. The sex of the horse did not affect significantly the Se, Cu and Zn content of the hair. The habitat in Bavaria and Lower Saxony influenced by different offer the Se and Cu state in hair significantly and supports the hypothesis that horse hair can indicate their status in the deficiency or surplus. The Zn contents in the horse hair of the two locations were almost identical. Diagnostically blood tests of Se, Cu and Zn reflect actual deficiency situations well, the hair analysis informs about possible supply disorders in longer periods. For Se and Cu analyses are mane and coat hairs preferable to the tail hair. Lower limits are noted for Se in white hair.Keywords: trace elements, supply status, horse hair, types of hair, habitats, Zitation: Ratjen A., Anke M., Fürll M. (2017) Selen-, Kupfer-und Zink-Gehalte in braunen, schwarzen sowie weißen Deck-, Mähnen-und Schweifhaaren bei Pferden zweier Standorte. Pferdeheilkunde 33, 59-65;

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Relationship between hair elements and severity of atrioventricular block in horses

Suzuki

Yamaya

Asano

et al. 2007

Biol Trace Elem Res

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The aim of this study was to investigate the relationships between the mean concentrations of trace elements and the severity of the second-degree atrioventricular (AV) block in the mane hair of horses. Electrocardiographs of horses were continually recorded for 6 h using a holter cardiac monitor to determine dropped ventricular beats (DVBs) which can be used as an indicator of the severity of the AV block. Mane hair Ca, Cu, Mg, and Zn concentrations were measured by the particle-induced X-ray emission method. The Zn/Cu ratio and Ca concentration in mane hair were significantly and positively correlated with the hourly DVBs in horse with a second-degree AV block (p<0.01, r2=0.485; p<0.05, r2=0.351, respectively). Proposed diagnostic cutoff points for hair Ca concentration and Zn/Cu ratio based on receiver operating characteristics curves analysis in detecting second-degree AV block were set at 1536 microg/g and 26.0, respectively. Those results with horse hair suggest that the evaluation of the Ca, Cu, Mg, and Zn status in mane hair by this method is strongly related to the severity of second-degree AV block and might predict the susceptibility of an individual much before the development of the symptom.

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Influence of the Coat Color on the Trace Elemental Status Measured by Particle-Induced X-ray Emission in Horse Hair

Cited by 20 publications

References 23 publications

Relationship between Trace Elements Status in Mane Hair and Atrial Fibrillation in Horse

Relationship between Trace Elements Status in Mane Hair and Atrial Fibrillation in Horse

Selenium, copper and zinc contents in brown, black and white mane, coat and tail hairs in horses of two locations

Relationship between hair elements and severity of atrioventricular block in horses

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