Influence of the charge of low molecular weight proteins on their efficacy of filtration and/or adsorption on dialysis membranes with different intrinsic properties

Moachon, N.; Boullanger, Christophe; Fraud, S.; Vial, E.; Thomas, M; Quash, G.

doi:10.1016/s0142-9612(01)00151-x

Cited by 40 publications

(20 citation statements)

References 27 publications

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“…Most studies addressing this issue were accomplished under simplified laboratory conditions (by means of phantoms and pre-fractionated plasma), [10][11][12][13][14] or targeted onto a predefined set of molecules. [15][16][17][18][19][20][21][22] However, to investigate the subject in its full complexity and in an unbiased manner, the elution of dialysers used in regular HD and in the proteomic approach is crucial. Therefore, the aim of our study was to verify the elution algorithm eligible for proteomic analysis, to highlight the eluate proteome, and to qualify the potential molecules involved in the blood-dialyser interaction.…”

mentioning

confidence: 99%

Specific adsorption of some complement activation proteins to polysulfone dialysis membranes during hemodialysis

Mareš

Thongboonkerd

Tůma

et al. 2009

Kidney International

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Dialyser bioincompatibility is an important factor contributing to complications of hemodialysis with well known systemic consequences. Here we studied the local processes that occur on dialysis membranes by eluting proteins adsorbed to the polysulfone dialyser membranes of 5 patients after 3 consecutive routine maintenance hemodialysis sessions. At the end of each procedure, a plasma sample was also collected. These eluates and their accompanying plasma samples were separated by 2-dimensional gel electrophoresis; all proteins that were present in all patients were analyzed by tandem mass spectrometry; and a ratio of the relative spot intensity of the eluate to plasma was calculated. Of 153 proteins detected, 84 were found in all patients, 57 of which were successfully identified by mass spectrometry as 38 components of 23 unique proteins. In 10 spots the relative eluate intensity differed significantly from that in the plasma, implying preferential adsorption. These proteins included ficolin-2, clusterin, complement C3c fragment, and apolipoprotein A1. Our finding of a selective binding of ficolin-2 to polysulfone membranes suggests a possible role of the lectin complement pathway in blood-dialyser interactions.

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mentioning

confidence: 99%

Specific adsorption of some complement activation proteins to polysulfone dialysis membranes during hemodialysis

Mareš

Thongboonkerd

Tůma

et al. 2009

Kidney International

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“…The adsorptive capacity for low-molecular-weight plasma proteins varies among different hemofilter membrane materials [35,56,57]. Adsorptive clearances for middle molecules are time-dependent, with maximum values occurring in the first few hours of therapy [58].…”

Section: Drug Adsorption Clearance In Continuous Renal Replacement Thmentioning

confidence: 99%

“…These membranes have high sieving coefficient/saturation coefficients (S c /S d ) for solutes in a wide range of molecular weights (0 to 20,000 daltons), and this further contributes to high drug and metabolite clearance [59,61]. Another aspect that influences adsorption is the membrane’s electrical charge [57]. Whereas polysulfone and polyamine hemofilters have no net charge, PAN filters have a negative charge.…”

Section: Effect Of Membrane Characteristicsmentioning

confidence: 99%

Treatment with echinocandins during continuous renal replacement therapy

2014

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Echinocandins are indicated as first-line treatment for invasive candidiasis in moderate to severe illness. As sepsis is the main cause of acute kidney injury, the combination of echinocandin treatment and continuous renal replacement therapy (CRRT) is common. Optimizing antibiotic dosage in critically ill patients receiving CRRT is challenging. The pharmacokinetics of echinocandins have been studied under various clinical conditions; however, data for CRRT patients are scarce. Classically, drugs like echinocandins with high protein binding and predominantly non-renal elimination are not removed by CRRT, indicating that no dosage adjustment is required. However, recent studies report different proportions of echinocandins lost by filter adsorption. Nevertheless, the clinical significance of these findings remains unclear.

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“…The alteration of  2 -microglobulin in plasma levels may not simply be a result of trans-membrane transport; the adsorption to the membrane may also play a role in the observed plasma changes (Hoenich & Katopodis, 2002). For the removal of  2 -microglobulin, cellulose derived membrane is impermeable to  2 -microglobulin due to its dense symmetrical structure which does not permit the easy diffusion or convection of proteins through the membrane, while polyacrylonitrile (PAN), polysulfone and polymethylmethacrylate (PMMA) membrane could be used (Moachona et al, 2002). The PMMA membrane could also adsorb  2 -microglobulin.…”

Section: Biocompatibilitymentioning

confidence: 99%