Influence of the age of the individual on the stability of boar sperm genetic material

Czubaszek, Magdalena; Andraszek, Katarzyna; Banaszewska, Dorota

doi:10.1016/j.theriogenology.2019.11.018

Cited by 17 publications

(15 citation statements)

References 52 publications

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“…Results show that the percentage of spermatozoa with fragmented nuclear DNA in young animals could decrease along with the aging of the boar being especially notorious until two years old. Therefore, these results support that the exploitation of young boars without testing their sperm nuclear DNA stability could bear to poor individual reproductive results, as previously suggested by [27]. Early diagnosis of sperm nuclear DNA fragmentation would allow for stricter selection and elimination of males with potential fertility disorders from breeding.…”

Section: Discussionsupporting

confidence: 83%

“…In the boar population of our study, tDFI ranged from 0.018 to 20.1% (LNtDFI: −4 to 3). The big number of randomly chosen ejaculates analyzed in the present study explains the bigger range of tDFI in comparison to other authors [27]. Didion et al (2009) [7] proposed that boar spermatozoa with greater than 6% DFI might negatively affect both the farrowing rate and total piglets born.…”

Section: Discussioncontrasting

confidence: 47%

“…Sperm concentration increases sharply from puberty to the first year of age, followed by a long-term moderate decrease until 3 years of age and it stabilizes thereafter [34]. Particularly for sperm fragmentation, [27] found that the age of the boar had a significant effect on DNA stability and chromatin structure. These authors found lower percentage of spermatozoa with DNA fragmentation in the group of boars aged 20-26 months old in comparison to the young animals (7-8 months old).…”

Section: Discussionmentioning

confidence: 96%

“…Regarding molecular sperm quality, while boar-spermatozoa collected in springsummer appeared to have a relatively higher percentage of spermatozoa with damaged nuclear DNA, a significant increase was only evident in fractionated ejaculates from two out of five boars [25]. Contrarily, Petrocelli et al (2015) [27] reported that season, photoperiod or genetic line did not affect sperm nuclear DNA fragmentation. However, Peña et al (2019) [14] described that a tropical summer induces nuclear DNA damage and reduces sperm concentration without depressing motility suggesting that traditional methods of evaluating sperm motility may not detect inherently compromised spermatozoa.…”

Section: Introductionmentioning

confidence: 87%

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Seasonal Changes of Nuclear DNA Fragmentation in Boar Spermatozoa in Spain

Ausejo

Martínez²,

Soler‐Llorens³

et al. 2021

Animals

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There are numerous cases when conventional spermiogram parameters are all within an acceptable range but boar subfertility persists. The total sperm nuclear DNA fragmentation index (tDFI) is a trait related to fertility and prolificacy problems that is not routinely evaluated in commercial AI boars. The aim of this research was to study the effect of the photoperiod, season and reproductive age of the boar on tDFI (measured by SCSA) of 1279 ejaculates from 372 different boars belonging to 6 different breeds located in 6 AI studs in Spain. tDFI data ranged from 0.018% to 20.1%. Although there was a significant single boar effect in the tDFI occurrence, a negative correlation between the tDFI and the age of the boar was found (p < 0.001). tDFI would decrease due to aging of the boar 0.66% each year old within the observed age range. After including age as a covariate in the ANCOVA, no differences were found in tDFI between photoperiods when the sperm collection date was evaluated. However, when the date of the production of semen in the testis was evaluated, the total percentage of spermatozoa with fragmented nuclear DNA was 1.46% higher in the increasing photoperiod in comparison to the decreasing photoperiod (p < 0.0001). On the other hand, for both dates, the lowest tDFI values corresponded to minimum day length for decreasing photoperiod phase (autumn), while the highest tDFI values were found in summer (maximum day length for decreasing photoperiod phase).

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Section: Discussionsupporting

confidence: 83%

Section: Discussioncontrasting

confidence: 47%

Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 87%

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Seasonal Changes of Nuclear DNA Fragmentation in Boar Spermatozoa in Spain

Ausejo

Martínez²,

Soler‐Llorens³

et al. 2021

Animals

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“…This indicates the high sensitivity of the comet assay and its potential for use as a biomarker to assess the impact of age on genome stability. Czubaszek et al [48] examined chromatin stability in boar sperm and also found a higher level of DNA damage (0.61%) in older boars used at sow insemination stations than in young animals. Hypo-and hypermethylation also increase the amount of damage.…”

Section: Plos Onementioning

confidence: 98%

Assessment of chromosome stability in boars

Wójcik

Sokół

2020

PLoS ONE

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Chromosome instability adversely affects animal fertility and reproduction. Analysis of instability can be a valuable diagnostic tool. Helpful tests for assessment of instabilities include the sister chromatid exchange assay, identification of fragile sites, the bleomycin assay and the comet assay. These techniques can be used to assess and compare the chromosome stability of individual breeds of animals. The aim of the study was to assess chromosome stability in boars: Duroc, Duroc x Pietrain and Pietrain x Duroc crossbreds, Polish Large White, and the Neckar, P76 and PIC lines. The study assessed the chromosome stability of boars. The distribution of instabilities in individual breeds was varied. The average frequency of chromatid exchange was 4.8 ± 1.5, while that of fragile sites was 3.9 ± 1.4. The mean level of DNA damage (% tail DNA) was 9.4 ± 8.3, while in the bleomycin assay b/c and %AM were 0.6 ± 0.7 and 44.4 ± 4.1. A higher rate of instability was found in older individuals than in younger ones. The cytogenetic assays used to identify various forms of chromosome instability can be used to evaluate boars intended for breeding.

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