Influence of TG5 and LEP gene polymorphism on quantitative and qualitative meat composition in beef calves

Седых, Т. А.; Калашникова, Л. А.; Gusev, I.V.; Pavlova, I. Y. U.; Gizatullin, R. S.; Dolmatova, I. Yu.

doi:10.33899/ijvs.2016.121382

Cited by 15 publications

(7 citation statements)

References 9 publications

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“…A similar conclusion was reached by Sedykh et al . [ 29 ], who observed a significant (p < 0.05) superiority of TG5 TT genotype carriers in terms of fat content in muscle tissue relative to their peers in Hereford bulls. However, they did not find significant differences between Hereford bulls of various genotypes regarding the LEP gene.…”

Section: Discussionmentioning

confidence: 99%

Effect of TG5 and LEP polymorphisms on the productivity, chemical composition, and fatty acid profile of meat from Simmental bulls

Sycheva,

Latynina,

Mamedov

et al. 2023

Vet World

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Background and Aim: Enhancing the nutritional and biological value of meat obtained from young surplus replacement animals of dual-purpose breeds is a critical objective in the livestock industry. This study aimed to investigate the impact of thyroglobulin (TG5, c. −422C > T) and leptin (LEP, c. 239C > T) polymorphisms on the productivity, chemical composition, and fatty acid (FA) profile of meat from Simmental bulls. Materials and Methods: A total of 26 Simmental bulls were genotyped for TG5 (c. −422C > T) and LEP (c. 239C > T) polymorphisms and reared under the same fattening conditions. Controlled slaughter was conducted at 18 months of age. Subsequently, the experimental animals were evaluated to determine their slaughter traits and the chemical and FA composition of ground beef and the longissimus dorsi muscle. Results: The results showed that the TG5 (c. −422C > T) polymorphism significantly (p < 0.05) affected the differentiation of bulls in terms of the synthesis of stearic acid, linolenic acid, and total polyunsaturated FAs, as well as the fat and dry matter content in the longissimus dorsi muscle. Conversely, the presence of the T allele in the LEP (c. 239C > T) polymorphism was associated with increased dry matter and fat in ground beef, carcass weight, and internal fat weight. Conclusion: The analysis of slaughter traits and the chemical and FA composition of meat from the Simmental bulls genotyped for the TG5 and LEP genes revealed a genetic basis for the quantitative and qualitative characteristics of meat productivity. Thus, the genetic variability of bulls regarding the LEP and TG5 genes can be used to improve the quantitative and qualitative indicators of meat productivity in Simmental cattle through marker-assisted selection. Keywords: chemical composition, fatty acid, leptin, meat productivity, polymorphism, Simmental, thyroglobulin.

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Section: Discussionmentioning

confidence: 99%

Effect of TG5 and LEP polymorphisms on the productivity, chemical composition, and fatty acid profile of meat from Simmental bulls

Sycheva,

Latynina,

Mamedov

et al. 2023

Vet World

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“…Между тем применение традиционных методов селекции, основанных на всесторонней оценке животного, не всегда даёт желаемый результат, так как на определённом уровне темпы повышения продуктивности снижаются. Следовательно, использование в селекционном процессе сочетания селекционно-племенных методов и молекулярно-генетических обозначит качественно новый этап преобразований скота мясных пород (Tait RGJr et al, 2014;Sedykh TA et al, 2016).…”

Section: обсуждение полученных результатовunclassified

Breeding peculiarities of Hereford cattle of the intra-breed type Dmitrievsky, North Caucasian population, taking into account polymorphism GH (L127V) and LEP/A80V

Дубовскова¹

2020

AHFP

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Особенности селекции скота герефордской породы внутрипородного типа Дмитриевский Северо-Кавказской популяции с учётом полиморфизма GH (L127V) и LEP/А80V М.П. Дубовскова Федеральный научный центр биологических систем и агротехнологий Российской академии наук (г. Оренбург) Аннотация. Особенности селекционного процесса при совершенствовании внутрипородного типа Дмитриевский герефордской породы Северо-Кавказской популяции заключаются в сочетании традиционных методов отбора и подбора с молекулярно-генетическими, определяющими взаимодействие функциональной изменчивости с желательными аллелями генов. Цель исследования-изучение продуктивных и племенных качеств генеалогической группы животных внутрипородного типа Дмитриевский, полиморфизма ген-маркеров продуктивности и его генетической структуры в динамике поколений. Подопытными животными являлись быки-производители в возрасте 2 лет, коровы-первотёлки, бычки в 15 мес., тёлки в 18 мес. Дмитриевского типа и их аналоги базового варианта селекции. Характеристику генетической структуры популяции устанавливали определением частоты эритроцитарных антигенных факторов по трём системам групп крови (EAA, EAB, EAC). Изучали полиморфизм и частоту желательных генотипов генов гормона роста-GH (L127V) и лептина-LEP/А80V у быков-производителей, продолжателей генеалогических линий и родственных групп. Представители Дмитровского типа всех половозрастных групп отличались массивностью, лучшим развитием линейных промеров, молочностью по сравнению со сверстниками стада. Генетическое разнообразие Северо-Кавказской популяции герефордов с первого по третье поколение снижалось. Установлено снижение частоты антигена А2 на 33,0 %, I1-на 37 %, W-на 14,0 %. Отмечено привнесение на 13,0 % антигенного эритроцитарного фактора E ʹ 3 (локус EAВ). При изучении полиморфизма генов гормона роста (L127V) и лептина (А80V) установлена различная частота встречаемости отдельных генотипов. Гетерогенность популяции ассоциировалась с различиями по весовому росту в группе быков. При этом живая масса носителей генотипов VV и АА по генам GH и LEP превосходила класс элита на 5,62 % и на 2,9 %, носители генотипов LV и LL гена гормона роста-на 1-1,68 %, а генотипа AV лептина-на 0,78 %. Анализ показателей живой массы, молочности, промеров, статей экстерьера и выраженности типа телосложения показал преимущество Дмитриевского типа. Генотипирование быков-производителей свидетельствует о наличии тенденции взаимосвязи аллелей V и A в гомозиготной форме генов гормона роста и лептина с тяжеловесностью животных. Ключевые слова: крупный рогатый скот, герефордская порода, Дмитриевский тип, генетическая структура, живая масса, конституция и экстерьер, линейные промеры, частота антигенных факторов, однонуклеотидный полиморфизм GH L127V, LEP/A80V. UDC 636.082.11 (470.6) Breeding peculiarities of Hereford cattle of the intra-breed type Dmitrievsky, North Caucasian population, taking into account polymorphism GH (L127V) and LEP/A80V

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“…The leptin (LEP) gene is a promising gene in cattle breeding. It is characterized by a high degree of polymorphism and is associated with important functions, such as fat and energy metabolism, feeding behavior, milk productivity, feed efficiency, and reproductive ability [10][11][12]. The LEP gene has been identified on bovine chromosome 4.…”

Section: Introductionmentioning

confidence: 99%

Effect of leptin C528T and leptin C73T polymorphisms and pregnancy on adipose tissue formation and carcass grade in Aberdeen Angus heifers and first-calf cows

et al. 2022

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Background and Aim: The leptin (LEP) gene plays a role in the regulation of the activity required to obtain food, energy metabolism, and fat deposition and affects the body composition of animals. Lipogenesis is an ineffective process. A lot of energy from feed is expended on the synthesis of adipose tissue. This study aimed to determine the effect of LEP C528T and LEP C73T polymorphisms and pregnancy on adipose tissue formation and carcass grade in Aberdeen Angus heifers and first-calf cows. Materials and Methods: Heifers (n = 49) and first-calf cows (n = 30) were grouped according to their genotype for LEP C528T and LEP C73T polymorphisms at the age of 24 months. DNA samples were isolated from whole blood. Experimental animals were slaughtered at the age of 24 months, and a chemical analysis of samples of longissimus dorsi muscle and ground beef was performed. Results: The maximum internal fat weight, back-fat thickness, and fat content of the longissimus dorsi muscle and ground beef were determined in heifers heterozygous for both LEP C528T and LEP C73T polymorphisms. The ranking of genotypes in terms of adipose tissue formation did not change in first-calf cows compared to heifers carrying the same LEP gene variants. Pregnancy had a more significant (p < 0.05–0.001) effect on slaughter parameters and lipogenesis in animals of different genotypes than studied polymorphisms. Heterozygosity of the LEP gene was much more strongly expressed in the carcass grade of heifers. "Prime" grades were assigned to 66.7% of carcasses heterozygous for LEP C528T and "Top Choice" to 58.8% of carcasses with LEP C73T nucleotide substitutions. Conclusion: LEP C528T and LEP C73T polymorphisms were shown to affect the extent of fat formation in Aberdeen Angus heifers and first-calf cows. Animals with heterozygous genotypes exhibited the maximum development of internal fat, back-fat, and intramuscular fat. Pregnancy had a more significant effect on slaughter parameters and adipose tissue formation than studied polymorphisms. First-calf cows had a significantly lower fat content in carcasses than heifers. These results can aid in the production of efficient mature herds of Aberdeen Angus cattle.

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Influence of TG5 and LEP gene polymorphism on quantitative and qualitative meat composition in beef calves

Cited by 15 publications

References 9 publications

Effect of TG5 and LEP polymorphisms on the productivity, chemical composition, and fatty acid profile of meat from Simmental bulls

Effect of TG5 and LEP polymorphisms on the productivity, chemical composition, and fatty acid profile of meat from Simmental bulls

Breeding peculiarities of Hereford cattle of the intra-breed type Dmitrievsky, North Caucasian population, taking into account polymorphism GH (L127V) and LEP/A80V

Effect of leptin C528T and leptin C73T polymorphisms and pregnancy on adipose tissue formation and carcass grade in Aberdeen Angus heifers and first-calf cows

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