Abstract:This study aimed on evaluating the possible effects of tetracycline administered to rats on the tenth day of pregnancy, on kidney and liver development of their offspring. The liver showed vacuolization, necrosis, inflammation and sinusoidal dilatations, more evident in the newborn. Mitosis, early increase of Kupffer cells population and hipertrophy of hepatocytes with greater synthesis of glycogen were present on the tenth and twentieth days of life. The kidney showed slight tubular vacuolizations and necrosi… Show more
“…In contrast, both the mother and the baby cannot be harmed by the inadequate treatment of infections. [ 47 ] The present results showed that fetal liver from both treated groups revealed degeneration of hepatic tissue, fatty degeneration, congested central vein, necrosis, and lymphocyte invasion. In addition, the liver of the fetuses showed fatty changes, hemolysis of the blood in sinusoidal spaces, and central vein dilatation with focal hemosiderosis, necrosis, and pyknotic nuclei.…”
Section: Discussionmentioning
confidence: 50%
“…Another report showed that TC can cause chromosomal abnormalities. [ 47 ] In addition, the presence of mitochondrial rich placenta during pregnancy elevates oxidative stress [ 53,54 ] and alters many physiological and metabolic functions. [ 55 ] Over and above this, Sekhon et al [ 56 ] demonstrated that oxidative stress influences multiple physiological processes, from oocyte maturation to fertilization, embryo development, and pregnancy.…”
This experiment was performed to evaluate the possible embryotoxic and teratogenic effects of doxycycline during rat development. Twenty‐one female rats were used and distributed into three groups equally (seven animals/group). The low dose group received doxycycline at a dose of 5 mg/kg bw/day orally from the 6th to 14th day of gestation. The high dose group received 10 mg/kg bw/day orally for the same period, the Control group received 1 mL distilled water orally for the same period. The dams were dissected on the 20th day of gestation and their fetuses were subjected to morphological, skeletal, and histological examination. Moreover, DNA damage analysis of liver cells of pregnant rats and their fetuses or fetal skull was assessed by Comet assay. The obtained results showed a significant decrease in fetal body weight, several morphological anomalies, and severe lack of ossification on the skull bones, phalanges, and sternum bone as well as shortness in the ulna and radius bones. Histological studies of pregnant rats revealed congestion and dilatation of the central vein of the liver lobules and fatty degeneration of the hepatocytes. In addition, 20 day‐fetuses showed a marked increase of necrotic hepatocytes associated with an increased average of megakaryocytes and periportal leukocytic infiltration. Moreover, doxycycline induced a significant increase in the percentage of DNA damage and tail length of examined samples. Conclusively, doxycycline caused certain fetal abnormalities, so it is advisable to avoid using this drug during pregnancy.
“…In contrast, both the mother and the baby cannot be harmed by the inadequate treatment of infections. [ 47 ] The present results showed that fetal liver from both treated groups revealed degeneration of hepatic tissue, fatty degeneration, congested central vein, necrosis, and lymphocyte invasion. In addition, the liver of the fetuses showed fatty changes, hemolysis of the blood in sinusoidal spaces, and central vein dilatation with focal hemosiderosis, necrosis, and pyknotic nuclei.…”
Section: Discussionmentioning
confidence: 50%
“…Another report showed that TC can cause chromosomal abnormalities. [ 47 ] In addition, the presence of mitochondrial rich placenta during pregnancy elevates oxidative stress [ 53,54 ] and alters many physiological and metabolic functions. [ 55 ] Over and above this, Sekhon et al [ 56 ] demonstrated that oxidative stress influences multiple physiological processes, from oocyte maturation to fertilization, embryo development, and pregnancy.…”
This experiment was performed to evaluate the possible embryotoxic and teratogenic effects of doxycycline during rat development. Twenty‐one female rats were used and distributed into three groups equally (seven animals/group). The low dose group received doxycycline at a dose of 5 mg/kg bw/day orally from the 6th to 14th day of gestation. The high dose group received 10 mg/kg bw/day orally for the same period, the Control group received 1 mL distilled water orally for the same period. The dams were dissected on the 20th day of gestation and their fetuses were subjected to morphological, skeletal, and histological examination. Moreover, DNA damage analysis of liver cells of pregnant rats and their fetuses or fetal skull was assessed by Comet assay. The obtained results showed a significant decrease in fetal body weight, several morphological anomalies, and severe lack of ossification on the skull bones, phalanges, and sternum bone as well as shortness in the ulna and radius bones. Histological studies of pregnant rats revealed congestion and dilatation of the central vein of the liver lobules and fatty degeneration of the hepatocytes. In addition, 20 day‐fetuses showed a marked increase of necrotic hepatocytes associated with an increased average of megakaryocytes and periportal leukocytic infiltration. Moreover, doxycycline induced a significant increase in the percentage of DNA damage and tail length of examined samples. Conclusively, doxycycline caused certain fetal abnormalities, so it is advisable to avoid using this drug during pregnancy.
“…This may likely cause kidney failure due to prolonged rifampicin treatment of tuberculosis, as is clearly seen in our findings. [44] ; reported that rat offspring from parents that receive antibiotic treatment reveal some symptoms of tubular vacuolation and necrosis, and these symptoms are more pronounced in the newborn rats, although some signs of tubular restoration are also seen at 10 and 20 days after treatment. Our result regarding the rifampicin plus stem cell-treated kidney showed an apparently normal histological architecture of renal corpuscle with a well-formed glomerular tuft surrounded by Bowman's space.…”
IntroductionAnti-tuberculosis agent rifampicin is extensively used for its effectiveness. Possible complications of tuberculosis and prolonged rifampicin treatment include kidney damage; these conditions can lead to reduced efficiency of the affected kidney and consequently to other diseases. Bone marrow-derived mesenchymal stem cells (BMMSCs) can be used in conjunction with rifampicin to avert kidney damage; because of its regenerative and differentiating potentials into kidney cells. This research was designed to assess the modulatory and regenerative potentials of MSCs in averting kidney damage due to rifampicin-induced kidney toxicity in Wistar rats and their progenies. BMMSCs used in this research were characterized according to the guidelines of International Society for Cellular Therapy.MethodsThe rats (male and female) were divided into three experimental groups, as follows: Group 1: control rats (4 males & 4 females); Group 2: rats treated with rifampicin only (4 males & 4 females); and Group 3: rats treated with rifampicin plus MSCs (4 males & 4 females). Therapeutic doses of rifampicin (9 mg/kg/day for 3-months) and MSCs infusions (twice/month for 3-months) were administered orally and intravenously respectively. At the end of the three months, the animals were bred together to determine if the effects would carry over to the next generation. Following breeding, the rats were sacrificed to harvest serum for biochemical analysis and the kidneys were also harvested for histological analysis and quantification of the glomeruli size, for the adult rats and their progenies.ResultsThe results showed some level of alterations in the biochemical indicators and histopathological damage in the rats that received rifampicin treatment alone, while the control and stem cells treated group showed apparently normal to nearly normal levels of both bio-indicators and normal histological architecture.ConclusionsIntravenous administration of MSCs yielded sensible development, as seen from biochemical indicators, histology and the quantitative cell analysis, hence implying the modulatory and regenerative properties of MSCs.
“…11 Toxicity of TCs has proven by long use of this antibiotic or as high dose responses in which kidney and liver are seriously affected. [12][13][14] In their studies, they concluded that the renal side effects of tetracycline showed an increase in Fanconi's syndrome and blood urine nitrogen (BUN). In patients with pre-existing renal impairment, tetracycline may cause azotemia, hyperphosphatemia, and acidosis.…”
Section: Toxicity and Side Effects Of Tetracycline Antibioticmentioning
Tetracyclines have been used extensively as broad-spectrum antibiotics. They are effective against a wide variety of human diseases and animal caused by different microbial pathogens. The mode of Tetracycline action is reversibly inhibiting bacterial protein synthesis by binding with the prokaryotic 30S ribosomal subunit that prevents the association of aminoacyl-tRNA with the ribosomal target site and subsequently inhibits protein synthesis. Toxicity of tetracycline antibiotics was proven, by many studies, in which induction of fatty liver, acute pancreatitis, severe hepato-nephrotoxicity, contribute to the development and severity of microvesicular steatosis, increase in the triglycerides, AST, ALT, bilirubin, urea, creatinine and gamma-globulin levels. Histological analysis of liver samples of tetracycline-treated rats revealed high vacuolation of the cytoplasm of hepatic cells, sinusoidal dilation, hepatocellular necrosis and disappearance of the cell membrane in some hepatocytes. Tetracycline can also cause chromosomal abnormalities as well as interfere with male fertility.
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