Influence of test time on results of the impedance aggregometer Multiplate® analyser in dogs

Abid, Monia; Kalbantner, Kerstin; Mischke, R.

doi:10.1007/s00580-013-1795-4

Cited by 3 publications

(8 citation statements)

References 14 publications

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“…In dogs, 17 papers (LOE 3, Good–Fair) suggest efficacy of clopidogrel . Of these publications, 10 (all LOE 3, Good–Fair) suggest in vivo efficacy against provoked arterial thrombosis.…”

Section: Guidelinesmentioning

confidence: 99%

American College of Veterinary Emergency and Critical Care (ACVECC) Consensus on the Rational Use of Antithrombotics in Veterinary Critical Care (CURATIVE) guidelines: Small animal

Goggs

Blais

Brainard

et al. 2019

J Vet Emergen Crit Care

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Objectives: To systematically review available evidence and establish guidelines related to the risk of developing thrombosis and the management of small animals with antithrombotics. Design: Standardized, systematic evaluation of the literature (identified by searching Medline via PubMed and CAB abstracts) was carried out in 5 domains (Defining populations at risk; Defining rational therapeutic use; Defining evidence-based protocols; Refining and monitoring antithrombotic therapies; and Discontinuing antithrombotic therapies). Evidence evaluation was carried out using Population, Intervention, Comparison, Outcome generated within each domain questions to address specific aims. This was followed by categorization of relevant articles according to level of evidence and quality (Good, Fair, or Poor). Synthesis of these data led to the development of a series of statements. Consensus on the final guidelines was achieved via Delphi-style surveys.Draft recommendations were presented at 2 international veterinary conferences and made available for community assessment, review, and comment prior to final revisions and publication. Settings: Academic and referral veterinary medical centers.Results: Over 500 studies were reviewed in detail. Worksheets from all 5 domains generated 59 statements with 83 guideline recommendations that were refined during 3 rounds of Delphi surveys. A high degree of consensus was reached across all guideline recommendations. Conclusions:Overall, systematic evidence evaluations yielded more than 80 recommendations for the treatment of small animals with or at risk of developing thrombosis. Numerous significant knowledge gaps were highlighted by the evidence reviews undertaken, indicating the need for substantial additional research in this field. K E Y W O R D S ) for their dedicated contributions to this effort. We would also like to thank the members of the veterinary community who attended and contributed to the discussion of the guidelines during the 2018 EVECC Congress and IVECCS sessions and subsequently during the online open-comment stages. CONFLICT OF INTERESTThe authors declare that they have no conflicts of interest.

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“…In dogs, 17 papers (LOE 3, Good–Fair) suggest efficacy of clopidogrel . Of these publications, 10 (all LOE 3, Good–Fair) suggest in vivo efficacy against provoked arterial thrombosis.…”

Section: Guidelinesmentioning

confidence: 99%

American College of Veterinary Emergency and Critical Care (ACVECC) Consensus on the Rational Use of Antithrombotics in Veterinary Critical Care (CURATIVE) guidelines: Small animal

Goggs

Blais

Brainard

et al. 2019

J Vet Emergen Crit Care

View full text Add to dashboard Cite

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“…The concentration of agonists chosen was also consistent with the manufacturer's recommendations as well as previous studies (Marschner et al., ; Shipley et al., ). While it has been shown that higher concentrations of agonists lead to higher AUC values (Abid et al., ), it was not the goal of this study to maximize aggregation. We wanted to assess reference intervals on our Multiplate using standard methods.…”

Section: Discussionmentioning

confidence: 97%

“…We were able to successfully meet both aims. Previous studies have evaluated different anticoagulants, agonist concentrations, and durations of aggregation time for optimizing the Multiplate's measurement (Abid et al., ; Defontis et al., ; Marschner et al., ). We selected hirudin anticoagulant which allows for analysis of platelet function under physiologic calcium conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Limited information is available on expected platelet aggregation responses in healthy dogs using the Multiplate (Abid, Kalbantner, & Mischke, ; Defontis et al., ; Marschner et al., ). It has not been shown whether reference ranges, the interval between which 95% of values from a reference population will exist, are the same for all analyzers or vary by location and/or instrument.…”

Section: Introductionmentioning

confidence: 99%

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Establishment of reference ranges and evaluation of in vitro concentration‐dependent platelet inhibition by acetylsalicylic acid for multiple electrode impedance aggregometry in healthy dogs

Haines

Lee

Hegedus

et al. 2017

Vet Pharm & Therapeutics

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Acetylsalicylic acid (ASA, aspirin) is an antiplatelet medication used for prevention of thromboembolism. Effects of ASA appear to vary widely between dogs, but the underlying mechanisms are not understood. The Multiplate analyzer is a newer form of whole-blood impedance aggregometry recently validated for use in healthy dogs. A method utilizing this instrument to measure ASA effects on platelet function has not been established. The goals of this study were to establish reference ranges for the Multiplate in healthy dogs and secondly, to develop a technique to determine the in vitro concentration of ASA needed to cause 50% inhibition of platelet aggregation (IC50). Reference ranges established from 40 dogs at multiple test times for three agonists were consistent with previously published values. In vitro IC50 values were calculated using the sigmoid E model in 20 healthy dogs on two occasions to determine individual repeatability. Calculated in vitro IC50 demonstrated four ASA response groups: responder (n = 16), poor responder (n = 1), variable responder (n = 2), and nonresponder (n = 1). Multiplate within-assay variability was <10% for area under the curve (AUC), and between-assay baseline AUC variability was <15%. The described technique allowed for determination of an in vitro IC50 for ASA in dogs using a multiple electrode impedance aggregometer.

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“…In dogs, 11 papers (LOE 3, Good), and 6 papers (LOE 3, Fair), suggest efficacy of clopidogrel. Of these publications, 10 papers suggest in vivo efficacy against provoked arterial thrombosis, while 7 publications suggest clopidogrel has significant platelet inhibitory effects ex vivo using tests such as platelet aggregometry.…”

Section: Pico Question: Clopidogrel Therapymentioning

confidence: 99%

Consensus on the Rational Use of Antithrombotics in Veterinary Critical Care (CURATIVE): Domain 3—Defining antithrombotic protocols

Blais

Bianco

Goggs

et al. 2019

J Vet Emergen Crit Care

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Objectives:To systematically examine the evidence for use of a specific protocol (dose, frequency, route) of selected antithrombotic drugs, in comparisons to no therapy or to other antithrombotic therapies, to reduce the risk of complications or improve outcomes in dogs and cats at risk for thrombosis. Design: Standardized, systematic evaluation of the literature, categorization of relevant articles according to level of evidence (LOE) and quality (Good, Fair, or Poor), and development of consensus on conclusions via a Delphi-style survey for application of the concepts to clinical practice. Settings: Academic and referral veterinary medical centers. Results: Databases searched included Medline via PubMed and CAB abstracts. Eight different antithrombotic drugs were investigated using a standardized Patient, Intervention, Comparison, Outcome (PICO) question format both for dogs and cats, including aspirin, clopidogrel, warfarin, unfractionated heparin (UFH), dalteparin, enoxaparin, fondaparinux, and rivaroxaban, generating a total of 16 worksheets. Most studies identified were experimental controlled laboratory studies in companion animals (LOE 3) with only four randomized controlled clinical trials in companion animals (LOE 1). Conclusions:Overall, evidence-based recommendations concerning specific protocols could not be formulated for most antithrombotic drugs evaluated, either because of the wide range of dosage reported (eg, aspirin in dogs) or the lack of evidence in the current literature. However, clopidogrel administration in dogs and cats at risk of arterial thrombosis, notably in cats at risk of cardiogenic thromboembolism, is supported by the literature, and specific protocols were recommended. Comparably, aspirin should not be used as a sole antithrombotic in cats with cardiomyopathy. Using the available safety profile information contained in the literature, the panel reached consensus on suggested dosage schemes for most antithrombotics. Significant knowledge gaps were highlighted, which will hopefully drive novel research.

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Influence of test time on results of the impedance aggregometer Multiplate® analyser in dogs

Cited by 3 publications

References 14 publications

American College of Veterinary Emergency and Critical Care (ACVECC) Consensus on the Rational Use of Antithrombotics in Veterinary Critical Care (CURATIVE) guidelines: Small animal

American College of Veterinary Emergency and Critical Care (ACVECC) Consensus on the Rational Use of Antithrombotics in Veterinary Critical Care (CURATIVE) guidelines: Small animal

Establishment of reference ranges and evaluation of in vitro concentration‐dependent platelet inhibition by acetylsalicylic acid for multiple electrode impedance aggregometry in healthy dogs

Consensus on the Rational Use of Antithrombotics in Veterinary Critical Care (CURATIVE): Domain 3—Defining antithrombotic protocols

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