Influence of temperature on the rheological behavior of a new fucose-containing bacterial exopolysaccharide

Cruz, M.E.M.; Freitas, Filomena; Torres, Cristiana A.V.; Reis, Maria A.M.; Alves, Vítor D.

doi:10.1016/j.ijbiomac.2011.02.012

Cited by 26 publications

(40 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Temperature fluctuations did not have any effect on viscosity and viscoelasticity of the polymer solution even after few cycles of heating-cooling (Cruz et al, 2011).…”

Section: Non-gel-forming Bacterial Epsmentioning

confidence: 88%

“…Solution behavior of the polysaccharides affects the intrinsic viscosity, critical overlap concentration of the molecule and finally, the coiling tendency of the polymer (Freitas et al, 2011). Gel-forming ability arises from stiffness of the polymer chains and ability to produce a three-dimensional network as a result of crosslinking and intermolecular bonding between ordered zones of the constituent chains or entanglements, sometimes in a specific ionic environment (Sutherland, 2001;Thomas et al, 2013;Banerjee and Bhattacharyya, 2012).…”

Section: Solubility Solution Rheology and Gelation Mechanism Of Bactmentioning

confidence: 99%

“…Due to excellent thermostable rheological behavior in aqueous solvent, Fucopol can serve as a comparable or even better alternative to Fucogel, xanthan gum, guar gum and alginate (Freitas et al, 2011). It has demonstrated shear-thinning behavior with recovery of viscosity as soon as shear rate was reduced.…”

Section: Gel-forming Bacterial Epsmentioning

confidence: 99%

“…Till date, only few bacterial species have been genetically engineered or subjected to chemical modification to obtain molecules with fine-tuned properties. Thus there is ample scope for entry of new high-performance exopolysaccharide of bacterial origin and creation of a specific market niche (Freitas et al, 2011;Vanhooren and Vandamme, 2000). A novel application of microbial exopolysaccharides is in the area of environment management through flocculation of suspended pollutant particles in wastewater and industrial effluent water.…”

Section: Future Scopementioning

confidence: 99%

See 3 more Smart Citations

Rheological Behavior and Pharmaceutical Applications of Bacterial Exopolysaccharides

2017

J App Pharm Sci

View full text Add to dashboard Cite

Over the last few decades, numerous bacterial exopolysaccharides have been extensively studied for their composition, structure, biosynthesis and functional properties, owing to their unique properties over traditional plant-derived gums and synthetic polysaccharides. They have been employed in food processing industries as rheology modifiers, gelling agents, in oil drilling and cement industries. But their full-fledged commercialization is yet to be achieved and their scope and possibilities in pharmaceutical field and optimized drug delivery are yet to be explored. The present review outlines, critically evaluates and summarizes the research outcomes on structure, solubility, solution behavior, rheological characterization, gelation mechanism and potential industrial and pharmaceutical applications of curdlan, polysaccharide 13140, Fucopol, levan, sphingans, succinoglycan and several other bacterial exopolysaccharides in their native form and also their derivatives. It also aims to identify and propose potential drug delivery systems where gelling and non gel-forming exopolysaccharides can be utilized successfully for development of stable, acceptable and effective dosage form.

show abstract

“…Temperature fluctuations did not have any effect on viscosity and viscoelasticity of the polymer solution even after few cycles of heating-cooling (Cruz et al, 2011).…”

Section: Non-gel-forming Bacterial Epsmentioning

confidence: 88%

Section: Solubility Solution Rheology and Gelation Mechanism Of Bactmentioning

confidence: 99%

Section: Gel-forming Bacterial Epsmentioning

confidence: 99%

Section: Future Scopementioning

confidence: 99%

See 2 more Smart Citations

Rheological Behavior and Pharmaceutical Applications of Bacterial Exopolysaccharides

2017

J App Pharm Sci

View full text Add to dashboard Cite

show abstract

“…DMEM was supplemented with 1.5 g l 21 penicillin and 0.025 U ml 21 streptomycin). For the direct magnetic capturing method, 500 ml MP-EPS and MP -EPS-22/8 with a concentration of 10 mg ml 21 were incubated with 500 ml of the crude extract, respectively, without any pre-treatment, for 20 min at room temperature. For the ATPE study, the particles were incubated for 40 min at room temperature in an ATPS prepared as described in §2.2.4.…”

Section: Crude Higg Extracts Purificationmentioning

confidence: 99%

An extracellular polymer at the interface of magnetic bioseparations

Dhadge

Morgado

Freitas

et al. 2014

J. R. Soc. Interface.

Self Cite

View full text Add to dashboard Cite

FucoPol, a fucose-containing extracellular polysaccharide (EPS) produced by bacterium Enterobacter A47 using glycerol as the carbon source, was employed as a coating material for magnetic particles (MPs), which were subsequently functionalized with an artificial ligand for the capture of antibodies. The performance of the modified MPs (MP-EPS-22/8) for antibody purification was investigated using direct magnetic separation alone or combined with an aqueous two-phase system (ATPS) composed of polyethylene glycol (PEG) and dextran. In direct magnetic capturing, and using pure protein solutions of human immunoglobulin G (hIgG) and bovine serum albumin (BSA), MP-EPS-22/8 bound 120 mg hIgG g 21 MPs, whereas with BSA only 10 + 2 mg BSA g 21 MPs was achieved. The hybrid process combining both the ATPS and magnetic capturing leads to a good performance for partitioning of hIgG in the desired phase as well as recovery by the magnetic separator. The MPs were able to bind 145 mg of hIgG g 21 of particles which is quite high when compared with direct magnetic separation. The theoretical maximum capacity was calculated to be 410 + 15 mg hIgG adsorbed g 21 MPs with a binding affinity constant of 4.3 Â 10 4 M 21. In multiple extraction steps, the MPs bound 92% of loaded hIgG with a final purity level of 98.5%. The MPs could easily be regenerated, recycled and re-used for five cycles with only minor loss of capacity. FucoPol coating allowed both electrostatic and hydrophobic interactions with the antibody contributing to enhance the specificity for the targeted products.

show abstract

Structural characterization, physicochemical and rheological characteristics of flaxseed gum in comparison with gum Arabic and xanthan gum

Safdar

Liu

et al. 2022

Food Measure

View full text Add to dashboard Cite

Influence of temperature on the rheological behavior of a new fucose-containing bacterial exopolysaccharide

Cited by 26 publications

References 23 publications

Rheological Behavior and Pharmaceutical Applications of Bacterial Exopolysaccharides

Rheological Behavior and Pharmaceutical Applications of Bacterial Exopolysaccharides

An extracellular polymer at the interface of magnetic bioseparations

Structural characterization, physicochemical and rheological characteristics of flaxseed gum in comparison with gum Arabic and xanthan gum

Contact Info

Product

Resources

About