Influence of temperature and salinity on Ostreopsis cf. ovata growth and evaluation of toxin content through HR LC-MS and biological assays

Pezzolesi, Laura; Guerrini, Franca; Ciminiello, Patrizia; Dell’Aversano, Carmela; Iacovo, Emma Dello; Fattorusso, Ernesto; Forino, Martino; Tartaglione, Luciana; Pistocchi, Rossella

doi:10.1016/j.watres.2011.10.029

Cited by 101 publications

(86 citation statements)

References 36 publications

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“…The effects of salinity on the ecologically similar genus Ostreopsis are quite variable, ranging from negative to positive correlations with growth and toxicity (Pistocchi et al 2011). A Mediterranean O. ovata isolate displays optimal growth rates at high salinity (36−40), but toxicity is highest at salinity 32 (Pezzolesi et al 2012). While there is an evident lack of consistency in the responses of these various HAB species to changing salinity, species-specific increases or decreases in toxicity and growth rate are commonly reported and may become important to consider under future changing precipitation and eva poration regimes in coastal areas and estuaries.…”

Section: Salinitymentioning

confidence: 99%

Global change and the future of harmful algal blooms in the ocean

Fu¹,

Tatters²,

Hutchins³

2012

Mar. Ecol. Prog. Ser.

224

138

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The frequency and intensity of harmful algal blooms (HABs) and phytoplankton community shifts toward toxic species have increased worldwide. Although most research has focused on eutrophication as the cause of this trend, many other global-and regional-scale anthropogenic influences may also play a role. Ocean acidification (high pCO 2 /low pH), greenhouse warming, shifts in nutrient availability, ratios, and speciation, changing exposure to solar irradiance, and altered salinity all have the potential to profoundly affect the growth and toxicity of these phytoplankton. Except for ocean acidification, the effects of these individual factors on harmful algae have been studied extensively. In this review, we summarize our understanding of the influence of each of these single factors on the physiological properties of important marine HAB groups. We then examine the much more limited literature on how rising CO 2 together with these other concurrent environmental changes may affect these organisms, including what is possibly the most critical property of many species: toxin production. New work with several diatom and dinoflagellate species suggests that ocean acidification combined with nutrient limitation or temperature changes may dramatically increase the toxicity of some harmful groups. This observation underscores the need for more in-depth consideration of poorly understood interactions between multiple global change variables on HAB physiology and ecology. A key limitation of global change experiments is that they typically span only a few algal generations, making it difficult to predict whether they reflect likely future decadal-or century-scale trends. We conclude by calling for thoughtfully designed experiments and observations that include adequate consideration of complex multivariate interactive effects on the long-term responses of HABs to a rapidly changing future marine environment.

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Section: Salinitymentioning

confidence: 99%

Global change and the future of harmful algal blooms in the ocean

Fu¹,

Tatters²,

Hutchins³

2012

Mar. Ecol. Prog. Ser.

224

138

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“…To check if the increase in pellet weight is only due to the mucus presence or to the increase in cell number the counting was repeated after cellular chemical disaggregation by adding HCl 4 mm final concentration [25,31]. Several O. ovata a cultures were grown in a total volume of 200 mL for 30 days at 19°C and 24°C.…”

Section: Resultsmentioning

confidence: 99%

“…Since Ostreopsis spp. are benthic dinoflagellate that grow in lumps and segregate a mucous substance the homogenization of the suspension is really hard and some chemical disaggregation is necessary [25,31]. If only the number of cells is measured, the chemical disaggregation with HCl is a useful tool, but when the cultures integrity is important the acidification could be a problem.…”

Section: Discussionmentioning

confidence: 99%

“…strain studied has been suggested [43]. Under controlled conditions high growth was observed at 20°C although higher toxin production was obtained at 25°C [31], the same as Ostreopsis lenticularis that produces more toxin at highest temperatures [44]. In addition, it is not clear the influence of the salinity in the toxin production [26,31].…”

Section: Discussionmentioning

confidence: 99%

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Warm Seawater Microalgae: Growth and Toxic Profile of Ostreopsis Spp. from European Coasts

Fernández-Araujo¹,

Alfonso²,

Antelo³

et al. 2013

Oceanography

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“…Another high sensitivity bioassay of PSP and ASP at the nM level was developed based on the fluorimetric detection of [Ca 2+ ] in rat cortical primary cultures under the electrical field stimulation [58]. The haemolysis of sheep erythrocytes was used as a rapid and sensitive method to detect palytoxin in water samples, a good correlation was found between haemolytic assays and the total toxin content measured through HR-LC/MS [59]. Cell-based mouse neuroblastoma assay (MNA) performed favorably over the mouse bioassay because of its higher sensitivity and less time (4 hr vs. 48 hr) [60].…”

Section: Established and Newly Developed Bioassays For Algal Toxinsmentioning

confidence: 99%

Current Techniques for Detecting and Monitoring Algal Toxins and Causative Harmful Algal Blooms

2014

J Environ Anal Chem

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The detection and monitoring techniques for algal toxins and the causative harmful algal blooms (HABs) are essential for the protection of aquatic lives, shellfish safety, drinking water quality, and public health. Toward the development of fast, easy, and reliable techniques, much progress has been made during the last decade for the qualitative and quantitative analysis of algal toxins. This review highlights the recent progress and new trends of these analytical and monitoring tools, ranging from in-situ quick screening protocols for the monitoring of algal blooms to mass spectrometric analysis of trace levels of various algal toxins and structural elucidation. Solid-phase adsorption toxin tracking (SPATT) deployed in the field for the passive sampling of algal toxins has been recently validated, and improved ELISA-based methods with lower detection limits for more toxins have become commercially available for both screening and routine monitoring purposes. Liquid chromatography-mass spectrometry with several recent mass spectrometric innovations has expanded our understanding of traditional toxins, their metabolites along with newly discovered toxins of ecological importance. Several established in vivo and in vitro bioassays will continue to be used as benchmark toxicological testing of algal toxins; however, newly emerged molecular probing techniques such as real-time quantitative polymerase chain reaction (qPCR) have extended our ability to trace algal toxins from causative organisms at the molecular level. New chemical and biological sensors, lab-on-chip and remote sensing of blooms being developed will hold promise for early warning and routine monitoring to better manage and protect our freshwater, coastal and marine resources from adverse impact by harmful algal blooms.

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Influence of temperature and salinity on Ostreopsis cf. ovata growth and evaluation of toxin content through HR LC-MS and biological assays

Cited by 101 publications

References 36 publications

Global change and the future of harmful algal blooms in the ocean

Global change and the future of harmful algal blooms in the ocean

Warm Seawater Microalgae: Growth and Toxic Profile of Ostreopsis Spp. from European Coasts

Current Techniques for Detecting and Monitoring Algal Toxins and Causative Harmful Algal Blooms

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