Influence of sugars on enantioselective reduction using Saccharomyces cerevisiae in organic solvent

Albuquerque, Patrícia Melchionna; Witt, Maria Alice; Stambuk, Boris U.; Nascimento, Maria da Graça

doi:10.1016/j.procbio.2006.07.034

Cited by 11 publications

(1 citation statement)

References 40 publications

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“…Currently, several procedures are available for the preparation of (S)-EHB, such as whole cell catalytic bioreduction of EOB [2][3][4][5][6][7] or PEG-acetoacetate [8] by the actions of alcohol dehydrogenase (ADH) and glucose-6-phosphate dehydrogenase (G6PDH), chemically ethanolytic depolymerisation of poly[(R)-3-hydroxybutyrate] [9], asymmetric hydrogenation of EOB with chiral catalyst Ru-BINAP, or recombinant enzyme ADH catalyzed reduction of EOB in the presence of both formate dehydrogenase and cofactors (NAD + and NADP + ) [1]. Among these methods, whole cell catalyzed bioreduction with yeast is the most appealing means due to its cost eVectiveness [10].…”

Section: Introductionmentioning

confidence: 99%

Effects of industrial storage on the bioreduction capacity of brewer’s yeast

Yu¹,

Hou²,

Gong³

et al. 2008

J Ind Microbiol Biotechnol

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The effects of industrial storage on the changes of the cell viability and the activities of intracellular alcohol dehydrogenase (ADH) and glucose-6-phosphate dehydrogenase (G6PDH) in brewer's yeast, and the corresponding capacity for the bioconversion of ethyl-3-oxobutanoate (EOB) to ethyl (S)-3-hydroxybutanoate ((S)-EHB), were investigated. The viability of fresh brewer's yeast cells stored in industrial circulating cooling water at 1-2 degrees C showed 4 and 15% drop after the storage of 7 and 15 days, respectively, after which cells died rapidly. The pretreatment of the stored brewer's yeast cells by washing and screening significantly enhanced cell viability during industrial storage. The intracellular levels of ADH and G6PDH after permeabilization of these stored cells with cetyltrimetylammonium bromide (CTAB) were much higher, which showed only slight decrease within 2 weeks during the industrial storage. When the stored cells after the permeabilization treatment was used as the biocatalyst at 90-120 g/L, EOB was converted almost completely into enantiopure (S)-EHB with an enantiomeric excess (ee) more than 99% and a yield of over 96%, by fed-batch bioconversion of 560 mM EOB within 6 h.

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Section: Introductionmentioning

confidence: 99%

Effects of industrial storage on the bioreduction capacity of brewer’s yeast

Yu¹,

Hou²,

Gong³

et al. 2008

J Ind Microbiol Biotechnol

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Current awareness on yeast

2007

Yeast

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In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on yeasts. Each bibliography is divided into 10 sections. 1 Reviews; 2 General; 3 Biochemistry; 4 Biotechnology; 5 Cell Biology; 6 Gene Expression; 7 Genetics; 8 Physiology; 9 Medical Mycology; 10 Recombinant DNA Technology. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted. (4 weeks journals ‐ search completed 30th. May 2007)

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Asymmetric Reduction of Alkyl-3-oxobutanoates by Candida parapsilosis ATCC 7330: Insights into Solvent and Substrate Optimisation of the Biocatalytic Reaction

Venkataraman

Roy

Chadha

2013

Appl Biochem Biotechnol

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Asymmetric reduction of alkyl-3-oxobutanoates mediated by Candida parapsilosis ATCC 7330 resulted in optically pure alkyl-3-hydroxybutanoates in good yields (up to 72%) and excellent enantiomeric excess (up to >99 %). A detailed and systematic optimisation study was necessary and was carried out to avoid the undesired transesterification reaction during the course of asymmetric reduction. Under optimised conditions, the (S)-alkyl hydroxyesters were produced predominantly except for the methyl ester which formed the (R)-enantiomer. To the best of our knowledge, the biocatalytic asymmetric reduction of isoamyl-3-oxobutanoate to (S)-isoamyl-3-hydroxybutanoate is reported here for the first time.

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Influence of sugars on enantioselective reduction using Saccharomyces cerevisiae in organic solvent

Cited by 11 publications

References 40 publications

Effects of industrial storage on the bioreduction capacity of brewer’s yeast

Effects of industrial storage on the bioreduction capacity of brewer’s yeast

Current awareness on yeast

Asymmetric Reduction of Alkyl-3-oxobutanoates by Candida parapsilosis ATCC 7330: Insights into Solvent and Substrate Optimisation of the Biocatalytic Reaction

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