Influence of Sucrose Concentration on the Photosynthetic Ability of in Vitro Grown Rose Shoots

Langford, P.J.; Wainwright, H.

doi:10.17660/actahortic.1988.227.56

Cited by 13 publications

(7 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The highest photosynthesis rates were observed with plantlets grown on low (0 and 1%) sucrose. Capellades et al (1991) and Langford & Wainwright (1988) reported similar results with micropropagated roses. Cauliflower (Brassica oleracea var.…”

Section: Discussionsupporting

confidence: 64%

“…The inadequate gas exchange in culture vessels (Desjardins et al 1987), stomata deformation (Brainerd et al 1981) and the presence of sucrose in culture medium (Langford & Wainwright 1988) are factors associated with poor photosynthetic capacity of plantlets cultivated in vitro. Studies with micropropagated roses (Rosa multiflora L. cv.…”

Section: Introductionmentioning

confidence: 99%

“…Montse) showed that photosynthesis of plantlets was influenced by the level of sucrose in culture medium. Treatments with the low amounts of sucrose yielded the highest photosynthesis rates (Capellades et al 1991;Langford & Wainwright 1988). Phosphoenolpyruvate carboxylase (PEPC) is also important in CO 2 fixation in the multiplication and rooting stages of micropropagated Contribution No.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of sucrose on photosynthesis and phosphoenolpyruvate carboxylase activity of in vitro cultured strawberry plantlets

Hdider

Desjardins

1994

Plant Cell Tiss Organ Cult

108

View full text Add to dashboard Cite

Photosynthesis and phosphoenolpyruvate carboxylase activity were investigated in 5, 10 and 28 day-old micropropagated strawberry plantlets (Fragaria x ananassa Duch. cv Kent) rooted in vitro with different levels of sucrose (0, 1, 3 and 5%) on cellulose plugs (Sorbarods). The photosynthetic capability was influenced by the level of sucrose in the culture medium with the largest rates of photosynthesis corresponding to the cultures with 0 and 1% sucrose. The apparent quantum yield and the ratio of variable fluorescence to maximum fluorescence were also reduced in plantlets cultured with 3 or 5% sucrose as compared to those with 0 or 1%. Phosphoenolpyruvate carboxylase activity was largest 5 and 10 days after the onset of culture and decreased in the absence of sucrose in the culture medium. At 28 days after the onset of culture, the activity of this carboxylating enzyme was lower than at the beginning of culture and independent of the concentration of sucrose in the culture medium. Phosphoenolpyruvate carboxylase appears to be an important carboxylating enzyme in micropropagated plantlets.

show abstract

Section: Discussionsupporting

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of sucrose on photosynthesis and phosphoenolpyruvate carboxylase activity of in vitro cultured strawberry plantlets

Hdider

Desjardins

1994

Plant Cell Tiss Organ Cult

108

View full text Add to dashboard Cite

show abstract

“…Regarding roses, it has been found [4,10] that the higher the level of sucrose in the medium, the less CO 2 was taken up. In a study using wheat plants grown in a cabinet it was suggested that the accumulation of soluble sugars may reduce the rate of ribulose 1,5-bisphosphate carboxylase regeneration [2].…”

Section: Introductionmentioning

confidence: 99%

Effects of sucrose on starch accumulation and rate of photosynthesis in Rosa cultured in vitro

Capellades

Lemeur

Debergh

1991

Plant Cell Tiss Organ Cult

112

View full text Add to dashboard Cite

Shootlets of Rosa multiflora L. cv. Montse were cultured in vitro with four different levels of sucrose (0, 1, 3 and 5%). Chloroplasts of shootlets grown in a medium without sucrose contained numerous, large plastoglobuli and were lacking in starch granules. The size and number of starch granules increased with the level of sucrose in the culture medium. Starch content in leaves of shootlets grown with 5% sucrose was higher (ca 1, 3%) than those grown with 3% (ca 0, 45%) and 1% sucrose (ca 0, 27%). Starch might be used by the in vitro shootlets during the acclimation period.

show abstract

“…Basal medium, sucrose concentration and the interaction between them had significantly different effects on the number of roots per plantlet and root length (P \ 0.0001; Table 7). Note however, that the conditions of the shoots varied at different sucrose levels according to Langford and Wainwright (1988) and Al-Khalifah et al (2005). When no sugar or 10 g l -1 was used, the shoots tended to become necrotic, while in the presence of 30 g l -1 there was slight callusing.…”

Section: Rooting Inductionmentioning

confidence: 99%

Crambe tataria: actions for ex situ conservation

2010

View full text Add to dashboard Cite

In vitro micropropagation by direct organogenesis and somatic embryogenesis via callus was developed for Crambe tataria (Brassicaceae). C. tataria is an endemic species of the Pontic-Pannonic region, but it is also present in Italy, where it is localized in Friuli on a characteristic grassland formation, called "magredi". C. tataria is regarded as an endangered species. Leaf and root explants were subjected to plant regulator treatments, which invoked different morphogenic responses. Leaf explants produced more callus than root explants and a higher amount of callus was obtained with 1 mg l-1 2,4-D in combination with 2 mg l-1 Kin. Somatic embryogenesis was obtained in calli maintained in a delayed subculture regime on media containing BAP in combination with NAA. Root explants cultured with BAP combined with NAA developed adventitious rosette shoots. Shoots rooted on half-strength MS media, and the number of roots per plantlet and their length were heavily dependent on sucrose content. The in vitro regenerated plantlets were acclimatized ex vitro and a mean of 50% of the plantlets survived and showed a true-to-type growth habit. This study describes the development of two in vitro micropropagation protocols, via direct organogenesis and via embryogenesis from callus, that are the basis for the application of in vitro tools for the establishment of basal collections with representative genetic diversity and for the long-term storage of plant genetic materia

show abstract

Influence of Sucrose Concentration on the Photosynthetic Ability of in Vitro Grown Rose Shoots

Cited by 13 publications

References 0 publications

Effects of sucrose on photosynthesis and phosphoenolpyruvate carboxylase activity of in vitro cultured strawberry plantlets

Effects of sucrose on photosynthesis and phosphoenolpyruvate carboxylase activity of in vitro cultured strawberry plantlets

Effects of sucrose on starch accumulation and rate of photosynthesis in Rosa cultured in vitro

Crambe tataria: actions for ex situ conservation

Contact Info

Product

Resources

About