Influence of substrates on the <i>in vitro</i> kinetics of steviol glucuronidation and interaction between steviol glycosides metabolites and UGT2B7

Chen, Junming; Xia, Yongmei; Zhang, Yandong; Zhang, Tong-Tong; Peng, Qingrui; Fang, Yun

doi:10.1080/09637486.2017.1373079

Cited by 4 publications

(4 citation statements)

References 33 publications

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“…Natural toxins can be produced through the accumulation of some chemical substances and elements that the animal gets from food [1], they come as residues from dilapidated cells or some vital processes in the body, causing sheep inside the body that acts as a substance that cannot be used in the metabolic processes and difficult to get rid of [2]. [3] indicated that the animal is able to get rid of these substances accumulated inside the body that are difficult to get rid of in the usual ways through a mechanism called Glucuronide, known as Glucuronoside [4]. Glucuronic acid binds with accumulated substances by means of a glycosidic bond and ultimately these bound substances are excreted, which have a higher solubility than the accumulated substances out of the body.…”

Section: Introductionmentioning

confidence: 99%

Constraints Affecting the Welfare of Domestic Sheep Grazing in the Natural Pasture

Elkhateeb,

Ebraheem,

Abdulateef

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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The sheep like other animals need more and more welfare to produce meat of good quality, Sheep welfare problems primarily arise from mutilations, lameness, transportation, and disease. With proper grazing, breeding, and husbandry practices, most health issues in sheep can be resolved or avoided. Grazing involves seeking and choosing feed as well as grabbing and consuming it. The manner in which ruminants or grazing animals grip and consume forages varies. These variations are connected to the sorts of fodder that various ruminants prefer. Heat stress, cold stress, weariness, prolonged thirst, prolonged hunger, hampered movement, movement restrictions, resting issues, social stress, pain, fear, and distress are the welfare types that sheep may encounter during caged, all issues due to shortage of welfare, this results in sheep inside the body that acts as a substance that cannot be used in the metabolic processes and is difficult to get rid of the animal is able to get rid of these substances accumulated inside the body that are difficult to get rid of the presence of natural toxins can be produced through the accumulation of some chemical substances and elements that the animal gets from the food. They come as residues from deteriorated cells or some vital processes in the body.

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Section: Introductionmentioning

confidence: 99%

Constraints Affecting the Welfare of Domestic Sheep Grazing in the Natural Pasture

Elkhateeb,

Ebraheem,

Abdulateef

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…In recent years, a large number of foods and supplements with the lipid bilayer and mitochondrial health regulatory effects to reduce the risk of disease have been reported, including flavone O-glycosides and flavone C-glycosides components [56]. Currently, there are studies that have confirmed the interaction between O-glycosides and UGT2B7 [57], but we have not found any report on the interaction between C-glycosides and UGT2B7. How-ever, the sites and applicability of these components in cell metabolism have not been well clarified.…”

Section: Discussionmentioning

confidence: 57%

“…Nutrients 2023, 15, x FOR PEER REVIEW 12 of 16 [57], but we have not found any report on the interaction between C-glycosides and UGT2B7. However, the sites and applicability of these components in cell metabolism have not been well clarified.…”

Section: Discussionmentioning

confidence: 61%

Properties of Dietary Flavone Glycosides, Aglycones, and Metabolites on the Catalysis of Human Endoplasmic Reticulum Uridine Diphosphate Glucuronosyltransferase 2B7 (UGT2B7)

Xu,

Lv,

Cui

et al. 2023

Nutrients

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Flavone glycosides, their aglycones, and metabolites are the major phytochemicals in dietary intake. However, there are still many unknowns about the cellular utilization and active sites of these natural products. Uridine diphosphate glucuronosyltransferases (UGTs) in the endoplasmic reticulum have gene polymorphism distribution in the population and widely mediate the absorption and metabolism of endogenous and exogenous compounds by catalyzing the covalent addition of glucuronic acid and various lipophilic chemicals. Firstly, we found that rutin, a typical flavone O-glycoside, has a stronger UGT2B7 binding effect than its metabolites. After testing a larger number of flavonoids with different aglycones, their aglycones, and metabolites, we demonstrated that typical dietary flavone O-glycosides generally have high binding affinities towards UGT2B7 protein, but the flavone C-glycosides and the phenolic acid metabolites of flavones had no significant effect on this. With the disposition of 4-methylumbelliferone examined by HPLC assay, we determined that 10 μM rutin and nicotifiorin could significantly inhibit the activity of recombinant UGT2B7 protein, which is stronger than isovitexin, vitexin, 3-hydroxyphenylacetic acid and 3,4-dihydroxyphenylacetic acid. In addition, in vitro experiments showed that in normal and doxorubicin-induced lipid composition, both flavone O-glycosides rutin and flavone C-glycosides isovitexin at 10 μM had no significant effect on the expression of UGT1A1, UGT2B4, UGT2B7, and UGT2B15 genes for 24 h exposure. The obtained results enrich the regulatory properties of dietary flavone glycosides, aglycones, and metabolites towards the catalysis of UGTs and will contribute to the establishment of a precise nutritional intervention system based on lipid bilayers and theories of nutrients on endoplasmic reticulum and mitochondria communication.

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“…Steviol was mainly surrounded by the active amino acids of ligand-UGT2B7, including Arg352, Leu347, Lys343, Phe339, Tyr354, Lys355, and Leu353. 85 These studies revealed the kinetic characteristics of enzymes as well as the optimum reaction conditions that could facilitate the in vitro synthesis of steviol glycoside and its mass production.…”

Section: Microbial Synthesis Of Steviol Glycosides and The Precursorsmentioning

confidence: 99%

Progress and Prospects of Natural Glycoside Sweetener Biosynthesis: A Review

Qu,

Liu,

et al. 2023

J. Agric. Food Chem.

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To achieve an adequate sense of sweetness with a healthy low-sugar diet, it is necessary to explore and produce sugar alternatives. Recently, glycoside sweeteners and their biosynthetic approaches have attracted the attention of researchers. In this review, we first outlined the synthetic pathways of glycoside sweeteners, including the key enzymes and rate-limiting steps. Next, we reviewed the progress in engineered microorganisms producing glycoside sweeteners, including de novo synthesis, whole-cell catalysis synthesis, and in vitro synthesis. The applications of metabolic engineering strategies, such as cofactor engineering and enzyme modification, in the optimization of glycoside sweetener biosynthesis were summarized. Finally, the prospects of combining enzyme engineering and machine learning strategies to enhance the production of glycoside sweeteners were discussed. This review provides a perspective on synthesizing glycoside sweeteners in microbial cells, theoretically guiding the bioproduction of glycoside sweeteners.

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Influence of substrates on the in vitro kinetics of steviol glucuronidation and interaction between steviol glycosides metabolites and UGT2B7

Cited by 4 publications

References 33 publications

Constraints Affecting the Welfare of Domestic Sheep Grazing in the Natural Pasture

Constraints Affecting the Welfare of Domestic Sheep Grazing in the Natural Pasture

Properties of Dietary Flavone Glycosides, Aglycones, and Metabolites on the Catalysis of Human Endoplasmic Reticulum Uridine Diphosphate Glucuronosyltransferase 2B7 (UGT2B7)

Progress and Prospects of Natural Glycoside Sweetener Biosynthesis: A Review

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