Influence of substrate concentration on kinetic parameters of alkaline phosphatase enzyme

Abstract

A laboratory study was conducted in the laboratory of the Department of Soil Science and Water Resources in Agriculture and Forestry College and in the Faculty of Environment laboratory with the aim of showing the effect of temperature on the activity and effectiveness of the phosphatase enzyme and the calculation of kinetic and thermodynamic parameters (maximum velocity Vmax and concentration of the subject substance Km) and for the sake of So, it was soil chosen from Zawita forest, with sandy clay loam texture under the cover of pine trees. The soil was treated with different concentrations of Substrate subject to the enzyme (0.0125, 0.025, 0.05, 0.075, 0.1) molar and under the influence of different temperatures (10,20,30,40,50) Cᵒ.The results showed. A clear effect of the subject substance on the activity of the enzyme, as the enzyme effectiveness increased with the increase of the added concentrations of the substance. The highest efficacy value for alkaline enzyme phosphatase was V / [S] (0.6) µg P-nitrophenol gm-1. Hr-1, which resulted from the effect of concentration (100) mmol of the subject substance. While the lowest alkaline phosphatase enzyme effectiveness value V / [S] was (0.2) µg P-nitrophenol gm-1. Hr-1, which resulted from the effect of the lowest concentration of the subject substance (12.5) mmol. The results indicated thatV max value of amounted to (227.27) µg P-nitrophenol gm-1. Hr -1. while the value of Km amounted to (38.52) mmuler. The results showed an increase in enzyme phosphatase activity by increasing the incubation temperature at concentration (0.05) molar of the substrate, the highest value of the activity of the enzyme phosphatase reached V (155.47) mg P-nitrophenol gm -1 hr -1, which occurred from soil incubation at The highest temperature used in the study is (50) Cᵒ, as it was observed that the values of phosphatase enzyme effectiveness continued to increase until the highest temperature used in the study.