Abstract:While the definition of a nanomaterial (NM) is mainly based on size, it is known that decreasing size can induce structural modifications to compensate for increased surface energy. Nevertheless, the influence of these structural modifications on NM toxicity, and in particular structural defects, is poorly studied mainly because of the difficulty in varying the crystallinity of a NM without changing any other morphological parameters. In this study, we used a single-walled alumino-germanate nanotube (Ge-imogol… Show more
“…Objects smaller than the spatial resolution (∼10 nm) can also be detected if they possess a strong light scattering signal. Dark–field hyperspectral imaging has already been used to study NP-organism interactions, e.g., in vitro interactions of NPs with cell; , or in vivo interactions of NPs with unicellular organisms such as protozoa, bacteria, − and green algae, , or in entire organisms such as fishes or worms. , Despite its demonstrated usefulness to provide information on NP location in cells and small organisms, DF-HSI has not yet been used to detect NPs in terrestrial plants.…”
Terrestrial plants can internalize and translocate nanoparticles (NPs). However, direct evidence for the processes driving the NP uptake and distribution in plants is scarce at the cellular level. Here, NP-root interactions were investigated after 10 days of exposure of Arabidopsis thaliana to 10 mg·L of negatively or positively charged gold NPs (∼12 nm) in gels. Two complementary imaging tools were used: X-ray computed nanotomography (nano-CT) and enhanced dark-field microscopy combined with hyperspectral imaging (DF-HSI). The use of these emerging techniques improved our ability to detect and visualize NP in plant tissue: by spectral confirmation via DF-HSI, and in three dimensions via nano-CT. The resulting imaging provides direct evidence that detaching border-like cells (i.e., sheets of border cells detaching from the root) and associated mucilage can accumulate and trap NPs irrespective of particle charge. On the contrary, border cells on the root cap behaved in a charge-specific fashion: positively charged NPs induced a higher mucilage production and adsorbed to it, which prevented translocation into the root tissue. Negatively charged NPs did not adsorb to the mucilage and were able to translocate into the apoplast. These observations provide direct mechanistic insight into NP-plant interactions, and reveal the important function of border cells and mucilage in interactions of plants with charged NPs.
“…Objects smaller than the spatial resolution (∼10 nm) can also be detected if they possess a strong light scattering signal. Dark–field hyperspectral imaging has already been used to study NP-organism interactions, e.g., in vitro interactions of NPs with cell; , or in vivo interactions of NPs with unicellular organisms such as protozoa, bacteria, − and green algae, , or in entire organisms such as fishes or worms. , Despite its demonstrated usefulness to provide information on NP location in cells and small organisms, DF-HSI has not yet been used to detect NPs in terrestrial plants.…”
Terrestrial plants can internalize and translocate nanoparticles (NPs). However, direct evidence for the processes driving the NP uptake and distribution in plants is scarce at the cellular level. Here, NP-root interactions were investigated after 10 days of exposure of Arabidopsis thaliana to 10 mg·L of negatively or positively charged gold NPs (∼12 nm) in gels. Two complementary imaging tools were used: X-ray computed nanotomography (nano-CT) and enhanced dark-field microscopy combined with hyperspectral imaging (DF-HSI). The use of these emerging techniques improved our ability to detect and visualize NP in plant tissue: by spectral confirmation via DF-HSI, and in three dimensions via nano-CT. The resulting imaging provides direct evidence that detaching border-like cells (i.e., sheets of border cells detaching from the root) and associated mucilage can accumulate and trap NPs irrespective of particle charge. On the contrary, border cells on the root cap behaved in a charge-specific fashion: positively charged NPs induced a higher mucilage production and adsorbed to it, which prevented translocation into the root tissue. Negatively charged NPs did not adsorb to the mucilage and were able to translocate into the apoplast. These observations provide direct mechanistic insight into NP-plant interactions, and reveal the important function of border cells and mucilage in interactions of plants with charged NPs.
“…The results were subjected to statistical calculations for means comparison using XLSTAT software. One-way analysis of variance (ANOVA) was used to test the significance of the results followed by post hoc tests (TUKEY HSD) [17].…”
a,b a laboratoire de Biotechnologie Microbienne, faculté des sciences et Techniques, université sidi Mohamed Ben abdellah, fès, Morocco; b centre universitaire régional d'interface, université sidi Mohamed Ben abdellah, fès, Morocco;
“…brassicacearum is studied for its interaction with A. thaliana 26 and for its biocontrol properties. 27 The adaptive response of this bacterium to heavy metals 28 and to nanoparticles 29 has been extensively studied, and its ability to secrete two different siderophore molecules has been recently reported. 30 We tested two shapes of anatase titania particles and investigated their biological activity under dark conditions by analyzing the TiO 2 NPs-bacteria interactions, as well as their impact on the iron content and prrF1 and prrF2 sRNA expression.…”
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