Influence of stretch on the target phenomenon in the tenotomized and immobilized gastrocnemius muscle of the rat

Reuck, Jacques De; Coster, W. De; Eecken, H. vander

doi:10.1007/bf00685359

Cited by 4 publications

(1 citation statement)

References 5 publications

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“…Secondary accumulation of dystrophin‐associated proteins also occurs in targets and targetoid phenomena in muscle after chronic denervation [10]. The target formations were first described as cytoplasmatic inclusions in denervated muscle fibres by Engel [11]; they also occur in muscle biopsies of patients with periodic paralysis, dermatomyositis, or in fibres of tenotomized muscle fixed in hyperextension [12]. Targets and targetoid phenomena are also found after upper neurone lesion [13] and in patients with multiple sclerosis [14].…”

Section: Introductionmentioning

confidence: 99%

Disturbed trafficking of dystrophin and associated proteins in targetoid phenomena after chronic muscle denervation

Fellenberg

Lin

Burgunder

2003

Neuropathology Appl Neurobio

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Dystrophin and associated proteins form a complex with an important role at the sarcolemma. Expression of this protein complex is highly regulated during development and regeneration. In order to better understand assembling patterns of these proteins, we have studied their expression in targetoid-like phenomena found in human muscle after chronic denervation, a situation known to give rise to abnormal protein trafficking. In eight biopsies of patients with chronic denervation, mainly resulting from amyotrophic lateral sclerosis, we found a number of targetoid phenomena. Selective accumulation of a number of sarcolemmal and sarcoplasmatic proteins occurred in targetoid phenomena. The larger majority of them contained gamma-sarcoglycan (gammaSG), but none contained the developmental heavy chain myosin isoform. In a series of 166 targetoid phenomena which could be studied with 17 different antibodies recognizing sarcolemmal and cytoplasmatic proteins, a high level of colocalization of gammaSG with desmin and alpha-actinin was found. Colocalization rate was much lower with other proteins, including other members of the dystrophin-associated protein complex. These data show that selective changes in expression of otherwise closely related proteins occur during disturbed trafficking leading to target formation. Because members of the dystrophin-associated protein complex do not accumulate in a similar fashion within targets, we suggest that a complex molecular control of gene expression and trafficking of this complex is involved after chronic muscle denervation.

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Section: Introductionmentioning

confidence: 99%

Disturbed trafficking of dystrophin and associated proteins in targetoid phenomena after chronic muscle denervation

Fellenberg

Lin

Burgunder

2003

Neuropathology Appl Neurobio

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Influence of isaxonine on reinnervation of the rat gastrocnemius muscle

Reuck¹,

Bleecker²,

Coster³

et al. 1987

Acta Neuropathol

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Isaxonine is reported to have a favourable effect on nerve regeneration. The histological and histochemical changes were compared in the gastrocnemius muscles of 20 rats, in which the left sciatic nerve was frozen-crushed for 10 s at the level of the buttock. The animals received a daily dose of intraperitoneal isaxonine and ten others the solvent only. After 8 weeks small type grouping was seen in rats receiving solvent only and large type grouping in rats treated with isaxonine. After 10 weeks a checkerboard pattern of the fibre types was observed in the muscles of animals receiving solvent only but fibre type grouping was present in those treated with isaxonine. This experiment indicates that isaxonine prevents synaptic remodelling by suppression of the redundant nerve endings from additional axons, which have regenerated more slowly, in making contact with motor endplates.

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Influence of isaxonine on the target phenomenon, muscle fibre size and neuromuscular junction in the tenotomized and denervated gastrocnemius muscle of the rat

Bleecker¹,

Coster²,

Reuck³

et al. 1986

Acta Neuropathol

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Isaxonine is reported to accelerate the rate of peripheral nerve regeneration, stimulate axonal sprouting and promote motor and sensory function recovery. The target phenomenon in muscle fibers is the morphological expression of increased stretch activity in a diseased muscle. It can be produced in the gastrocnemius muscle of the rat by tenotomy and is inhibited by simultaneous denervation. The influence of isaxonine on this experimental model was studied. Twenty Wistar rats were submitted to bilateral section of the Achilles tendon and section of the left sciatic nerve. In ten rats, treated with isaxonine, the qualitative and quantitative changes in the gastrocnemius muscle were compared with the control group over a period of 3 weeks. No statistical differences were observed in the occurrence and inhibition of the target phenomenon, the muscle fibre size and the neuromuscular junction. Isaxonine has no direct effect on a diseased muscle with an intact nerve supply, nor in the early stages after complete denervation.

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Influence of stretch on the target phenomenon in the tenotomized and immobilized gastrocnemius muscle of the rat

Cited by 4 publications

References 5 publications

Disturbed trafficking of dystrophin and associated proteins in targetoid phenomena after chronic muscle denervation

Disturbed trafficking of dystrophin and associated proteins in targetoid phenomena after chronic muscle denervation

Influence of isaxonine on reinnervation of the rat gastrocnemius muscle

Influence of isaxonine on the target phenomenon, muscle fibre size and neuromuscular junction in the tenotomized and denervated gastrocnemius muscle of the rat

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