When there are multiple key strata in the overburden of a deep coal seam and the surface subsidence coefficient after mining is small, this indicates that the overlying key strata fail to break completely after mining. On this occasion, stress is easily concentrated in the working face, which in turn leads to the occurrence of dynamic disasters such as rock bursts. This study adopted a comprehensive analysis method of field monitoring and numerical simulations to explore the influence of the key stratum on the evolution law of mining-induced stress in the working face. A distributed optical fiber sensor (DOFS) and a surface subsidence GNSS monitoring system were arranged inside and at the mouth of the ground observation borehole, respectively. According to the monitoring results of strain obtained from the DOFS, the height of the broken stratum inside the overlying strata was obtained and according to the monitoring results of surface subsidence, the surface subsidence coefficient was proven to be less than 0.1, indicating that the high key stratum is not broken completely, but enters a state of bending subsidence instead. In order to reveal the influence of the key stratum on the mining-induced stress of the working face, two 3DEC numerical models with and without the key stratum were established for a comparative analysis. As the numerical simulation results show, when there are multiple key strata in the overburden, the stress influence range and the stress concentration coefficient of the coal seam after mining are relatively large. The study revealed the working mechanism of rock burst accidents after large-scale mining and predicted the potential area with a rock burst risk after mining of the working face, which was verified by field investigations. The research results are of great guiding significance for the identification of the working mechanism of rock bursts in deep mining condition and for their prevention and control.