Influence of stabilizing agent and synthesis temperature on the optical properties of silver nanoparticles as active materials in surface plasmon resonance (SPR) biosensor

Synthesis of non-toxic, biocompatible, and colloidal stable silver nanoparticle using egg-white protein as capping and reducing agents for sustainable antibacterial application

“…The resulting solution was mixed with 6 ml of silver nitrate solution (1000 ppm) drop by drop with strong stirring (120 rpm) and the reaction mixture pH was set at 7.2 and thereaer kept under sunlight and at a temperature of 38 C to 40 C. The reaction mixture showed an instant color change from white to pale yellow with the sharp surface plasmon resonance (SPR: l max 430 nm) band within 2 min of sunlight exposer, which was an indication of the nanoparticles formation, which was further conrmed by UV-Vis spectroscopy examination (the detailed experimental setup is presented in Table 1). In order to optimize the maximum the nanoparticles formation, aliquots of the reaction mixture was collected at different time intervals (i.e., 5,10,15,30,45,60, and 95 min) and examined by UV-Vis spectroscopy. The synthesized nanoparticles were dialyzed to remove the un-bounded proteins from solution through dialysis tubing (at width 25 mm, MWCO 12 000 Da, Sigma, USA), the water was changed two times during 24 h dialysis period.…”

Bio-functionalization of phytogenic Ag and ZnO nanobactericides onto cellulose films for bactericidal activity against multiple drug resistant pathogens

“…1) respectively. The UV-visible spectroscopy is one of the ideal and sensitive tool to predict the formation of silver and zinc nanomaterials (Mahmudin et al, 2016). The intense absorption is due to the surface plasmon resonance which describes the collective excitation of electrons (Umadevi et al, 2012).…”

Bio-hybridization of nanobactericides with cellulose films for effective treatment against members of ESKAPE multi-drug-resistant pathogens

“…The initial confirmation of synthesis was achieved by UV-Visible spectrophotometry with maximum absorption at 408 nm. The UVvisible spectroscopy is one the reliable tool to confirm the formation of silver nanobactericides (Mahmudin et al, 2016). The maximum absorbance in the range of 100 to 800 nm is due to the surface plasmon resonance which defines the collective excitation of electrons (Azar and Mohebbi, 2013).…”