Influence of solution chemistry on the deposition and detachment kinetics of RNA on silica surfaces

Shen, Yun; Kim, Hyung-Seok; Tong, Meiping; Li, Qingyun

doi:10.1016/j.colsurfb.2010.09.018

Cited by 14 publications

(8 citation statements)

References 45 publications

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“…Derjaguin-Landau-Verwey-Overbeek (DLVO) theory (Derjaguin, 1941;Verwey, 1947) describes the attractive and repulsive forces between a colloidal particle and a surface. Several studies have investigated the attachment of colloidal particles to bare mineral surfaces in simple electrolyte solutions (Chowdhury et al, 2014a;Chang and Bouchard, 2013;Shen et al, 2011;Yi and Chen, 2011;Jiang et al, 2010;Elimelech, 2008, 2006) and the results appear to be in good qualitative agreement with DLVO theory.…”

Section: Introductionmentioning

confidence: 69%

The attachment of colloidal particles to environmentally relevant surfaces and the effect of particle shape

McNew

Kananizadeh

et al. 2017

Chemosphere

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Despite the prevalence of nonspherical colloidal particles, the role of particle shape in the transport of colloids is largely understudied. This study investigates the attachment of colloidal particles onto environmentally relevant surfaces while varying particle shape and ionic strength. Using quartz crystal microbalance and atomic force microscopy measurements, the role of particle shape was elucidated and possible mechanisms discussed. The attachment of both spherical and stretched polystyrene colloidal particles onto a smooth alginate-coated silica surface showed qualitative agreement with DLVO theory. Attachment onto a Harpeth humic acid (HHA) surface, however, significantly deviated from DLVO theory due to its high surface heterogeneity and extended confirmation from the silica surface. This extended confirmation provided increased potential for spherical particle entanglement, while the enlarged major axis of the stretched particles hindered their ability to attach. As ionic strength increased, the HHA layer condensed and provided less potential for spherical particle entanglement and therefore the selectivity for spherical particle attachment vanished. The findings presented in this study suggest that colloidal particle shape may play a complex and important role in predicting the transport of colloidal particles, especially in the presence of natural organic matter-coated surfaces.

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Section: Introductionmentioning

confidence: 69%

The attachment of colloidal particles to environmentally relevant surfaces and the effect of particle shape

McNew

Kananizadeh

et al. 2017

Chemosphere

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“…The improvement of the adsorption process of miRNA isolation. Calcium ions have been shown to be significantly helpful for enhancing the adsorption of nucleic acids on silica surfaces 10,11 . The presence of divalent cations (Ca 2+ ) in solutions can intercalate between the phosphate groups of polynucleotides and the negatively charged inorganic substrate, forming the salt bridge, which provides a strong electrostatic attraction of nucleic acids and silica surfaces 11 .…”

Section: Resultsmentioning

confidence: 99%

“…The presence of monovalent or divalent ions, pH and/or ionic strength in the media, and temperature are all the influence factors on the interaction of the nucleic acid with the silica surface. The presence of Ca 2+ in solutions is found that it greatly enhances the deposition behavior of RNA on silica surfaces 10 , and the efficiency of divalent cations (Ca 2+ and Mg 2+ ) is higher than Na + in promoting adsorption 11 . These positively charged ions can promote the formation of salt bridges between the negatively charged silica surface and the backbone of nucleic acids at high salt conditions.…”

Section: Introductionmentioning

confidence: 99%

“…Franchi et al 11 found that divalent cations (Ca 2+ and Mg 2+ > 1 mM) were more efficient than monovalent cations (Na + > 10 mM) in mediating the adsorption of nucleic acids on the negatively charged clay minerals. Shen et al 10 investigated the RNA deposition on bare silica surfaces was examined over a wide range of ionic strength in both NaCl (5–100 mM) and CaCl 2 (0.5–5 mM) at pH 6.0 and pH 8.0. They found that increasing solution ionic strength in both NaCl and CaCl 2 at both pH conditions would lead to greater deposition efficiencies of RNA.…”

Section: Introductionmentioning

confidence: 99%

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Improve sample preparation process for miRNA isolation from the culture cells by using silica fiber membrane

Chen

2020

Sci Rep

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In clinical applications of miRNAs, the purity and quality of the testing samples are very critical, especially the obtained tissue sample volume is limited. If the extracted miRNAs are contaminated or different in quality before analysis, it will increase the variance of the analysis result and make the medical information judgment incorrect and cannot be portable. Herein, we improved the commercially extraction kit by realizing the fundamental mechanism and hoped to serve finding optimal procedures for increasing the recovery of miRNAs extracted from cultured cells. In the adsorption process, the factors, like increasing the ethanol concentration or adding Ca2+, could influence the RNA adsorption were investigated. For the elution process, the effect caused by raising the elution temperature and raising the pH value of elution buffer was studied. Finally, the conditions for miRNA extraction are optimal modified by using a 65% (v/v) solution of ethanol in the adsorption process, and using TE buffer with the pH value of 8.0 and raising the temperature to 55 °C in the elution. According to the quantified results, the improved extraction kit can promote the recovery of endogenous miR-21 by about 6 times by using the optimal extraction conditions comparing with the miRNeasy Mini Kit.

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“…This leads to a concentration and consequential osmotic pressure difference in the solution, pushing nanotubes together and causing reagglomeration. 24,25 Depletion interaction received quite some attention in research, but disagreement among authors exists as whether to work above 3,14,23 or below 26,27 the CMC of surfactants. In any case, even in the absence of depletion interaction needlessly high surfactant amounts should be avoided for economic reasons and to avoid performance losses when used at a later stage in e.g.…”

Section: −4mentioning

confidence: 99%

Highly Efficient Hyperbranched CNT Surfactants: Influence of Molar Mass and Functionalization

et al. 2014

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End-group-functionalized hyperbranched polymers were synthesized to act as a carbon nanotube (CNT) surfactant in aqueous solutions. Variation of the percentage of triphenylmethyl (trityl) functionalization and of the molar mass of the hyperbranched polyglycerol (PG) core resulted in the highest measured surfactant efficiency for a 5000 g/ mol PG with 5.6% of the available hydroxyl end-groups replaced by trityl functions, as shown by UV−vis measurements. Semiempirical model calculations suggest an even higher efficiency for PG5000 with 2.5% functionalization and maximal molecule specific efficiency in general at low degrees of functionalization. Addition of trityl groups increases the surfactant−nanotube interactions in comparison to unfunctionalized PG because of π−π stacking interactions. However, at higher functionalization degrees mutual interactions between trityl groups come into play, decreasing the surfactant efficiency, while lack of water solubility becomes an issue at very high functionalization degrees. Low molar mass surfactants are less efficient compared to higher molar mass species most likely because the higher bulkiness of the latter allows for a better CNT separation and stabilization. The most efficient surfactant studied allowed dispersing 2.85 mg of CNT in 20 mL with as little as 1 mg of surfactant. These dispersions, remaining stable for at least 2 months, were mainly composed of individual CNTs as revealed by electron microscopy.

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Influence of solution chemistry on the deposition and detachment kinetics of RNA on silica surfaces

Cited by 14 publications

References 45 publications

The attachment of colloidal particles to environmentally relevant surfaces and the effect of particle shape

The attachment of colloidal particles to environmentally relevant surfaces and the effect of particle shape

Improve sample preparation process for miRNA isolation from the culture cells by using silica fiber membrane

Highly Efficient Hyperbranched CNT Surfactants: Influence of Molar Mass and Functionalization

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