Influence of Soil Traits on Polyphenols Level in Moltkia petraea (Tratt.) Griseb. (Boraginaceae)

Abstract

-The Illyric-Balkan endemic species Moltkia petraea (Tratt.) Griseb. is very interesting as a potential horticultural and medicinal plant. The aim of this study was to investigate soil conditions of M. petraea habitats, the phenolic content in plant parts, and the infl uence of soil properties on the phenolic contents. The results were evaluated using Spearman rank order correlations. Analyzed soil samples contained very low to intermediate levels of physiologically active phosphorus, but were very rich in potash. Organic matter content of soil was high. Phenolic compound content was higher in leaves than in fl owers or stems. The analyses showed that M. petraea possesses considerable quantities of phenolic compounds and has no specifi c demands for particular soil conditions. A negative correlation was found between soil phosphorus content and total phenols content in leaves and stems, and with the total phenolic acids content in fl owers. Organic matter in soil also found to have a negative infl uence on total tannins content in stems. Among the tested geographical locations, the Mljet population showed a higher degree of separation from the remaining locations.

OPTIMIZED DNA ISOLATION PROTOCOL ENABLES AN INSIGHT INTO MOLECULAR GENETIC BACKGROUND OF ENDEMIC Moltkia petraea (Tratt.) Griseb. FROM BOSNIA AND HERZEGOVINA

“…The content of biologically active compounds in plants depends on various factors: influence of soil traits, plant age, harvesting time, tissue type etc. (Končić et al, 2010, Kremer et al, 2016, Kolodziej and Sugier, 2013. Kremer et al (2016) analyzed biochemical properties of M. petraea and reported the highest content of polyphenols, flavonoids and phenolic acids in leaves, the highest content of tannins highest in flowers, with the lowest content of the analyzed bioactive compounds in stems.…”

“…comprises five species (Cohen, 2014), with Moltkia petraea (Tratt.) Griseb growing mostly in Mediterranean and Submediterranean regions (Kremer et al, 2016). This species is a dense, dwarf shrub, up to 40 cm high, with deep violet-blue tubular flowers, in simple forked or branching revolute cymes, blooming from spring well into the summer (Fernandes, 1972;Polunin, 1987).…”

“…However, the author reported that any attempt at getting an amplicon failed. So far, M. petraea from Bosnia and Herzegovina has only been analyzed for the influence of soil properties on phenolic compound accumulation (Kremer et al, 2016) but no data on its genetic diversity exist.…”

Phenolic compounds in two subspecies of Drypis spinosa L. (Caryophyllaceae) in Croatia

“…(Boraginaceae) had the highest TP content in flowers (Vuković Rodríguez et al 2016). Additionally, investigations of TP content in ten Moltkia petraea populations obtained by Kremer et al (2016) showed that TP content was the highest in leaves in six populations. But in another four populations the TP content was the highest in flowers.…”

“…For comparison, TP content ranged from 4.43% in stem to 12.41% in leaves of Satureja montana, and from 5.96% in stem to 20.04% in leaves of S. subspicata (Dunkić et al 2012). Additionally, TP content in Moltkia petraea ranged from 3.26% in stem to 6.61% in leaves (Kremer et al 2016).…”