Influence of Soil Temperature to the Pathosystem Strawberry-Verticillium

Schubert, Peter J.; Golldack, J.; Schwärzel, H.; Lentzsch, Peter

doi:10.17660/actahortic.2009.838.23

Cited by 2 publications

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“…This concurs with recent observations that the wilt disease of the oilseed rape (OSR) has been occurring more frequently in recent, warmer years (Siebold and von Tiedemann, 2012 ). The Verticillium wilt of strawberry crops is also affected by temperature increases, as was shown by an increase of damage occurring during warm conditions (Schubert et al, 2009 ). Considering the current trend in plant disease control toward sustainable agriculture and the urgent need for agricultural adaptation to climate change (Howden et al, 2007 ), environmentally friendly solutions for Verticillium wilt problems, such as biological control, are especially desirable.…”

Section: Introductionmentioning

confidence: 99%

Aerial Warfare: A Volatile Dialogue between the Plant Pathogen Verticillium longisporum and Its Antagonist Paenibacillus polymyxa

et al. 2017

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Verticillium wilt caused by Verticillium spp. results in severe yield losses in a broad range of crops. Verticillium outbreaks are challenging to control, and exacerbated by increases in soil temperatures and drought associated with global warming. Employing natural antagonists as biocontrol agents offers a promising approach to addressing this challenge. Paenibacillus polymyxa Sb3-1 was proven to reduce the growth of Verticillium longisporum during in vitro experiments and was shown to promote the growth of oilseed rape seedlings infested with V. longisporum. Our novel approach combined in vitro and in planta methods with the study of the mode of interaction between Sb3-1 and V. longisporum EVL43 via their volatile organic compounds (VOCs). Volatile and soluble substances, produced by both microorganisms as a reaction to one another's VOCs, were detected by using both gas and liquid chromatography-mass spectrometry. P. polymyxa Sb3-1 continually produced antimicrobial and plant growth promoting VOCs, such as 2-nonanone and 3-hydroxy-2-butanone. Several other antimicrobial volatile substances, such as isoamyl acetate and durenol, were downregulated. The general metabolic activity of Sb3-1, including protein and DNA biotransformations, was upregulated upon contact with EVL43 VOCs. V. longisporum increased its production of antimicrobial substances, such as 1-butanol, and downregulated its metabolic activities upon exposure to Sb3-1 VOCs. Additionally, several stress response substances such as arabitol and protein breakdown products (e.g., L-Isoleucyl-L-glutamic acid), were increased in the co-incubated samples. The results obtained depict an ongoing dialog between these microorganisms resulting in growth inhibition, the slowing down of metabolism, and the cell death of V. longisporum due to contact with the P. polymyxa Sb3-1 VOCs. Moreover, the results indicate that VOCs make a substantial contribution to the interaction between pathogens and their natural antagonists and have the potential to control pathogens in a novel, environmentally friendly manner.

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Section: Introductionmentioning

confidence: 99%

Aerial Warfare: A Volatile Dialogue between the Plant Pathogen Verticillium longisporum and Its Antagonist Paenibacillus polymyxa

et al. 2017

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“…Vitality rating led to results contradicting previous experiments in laboratory, greenhouse and field conducted in 2005 [24,25]. Consequently, evidence for a proof-ofconcept independently from spatial settings is not given.…”

Section: Discussionmentioning

confidence: 59%

“…The proposed measure is based on findings that relate wilt incidence to changes in plant microbial population structures and the observation that wilted plants are colonized by a different number and composition of subtypes with different pathogenicity compared to healthy plants. The BCA (EVI) consists of three non-pathogenic genotypes of Verticillium dahliae shown to successfully control wilt symptoms in strawberry plants in different climate regions [23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Field Application of Non-Pathogenic <i>Verticillium dahliae</i> Genotypes for Regulation of Wilt in Strawberry Plants

Diehl¹,

Rebensburg²,

Lentzsch³

2013

AJPS

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Verticillium dahliae induced wilt disease in strawberry can cause severe yield losses and thus lead to inevitable negative economic impacts. Inoculation of plants with non-pathogenic strains of Verticillium was conducted as a biologic control agent (BCA) according to the concept that preoccupation of the ecologic niche rendered strawberry plants immune to infection with soil-borne pathogenic Verticillium. This concept was tested for economic viability in a field trial under commercial conditions. Results were reported for 2 years of field trials under practice conditions in two locations in Brandenburg, Germany. Inoculation was shown to have a positive effect of 20% of plants, while 30% of plants remain unaffected and of equally high vitality. However, 50%-60% of plants were impacted negatively, showing severe wilt symptoms up to total loss. The characteristic progression of wilt symptoms suggested an infestation caused by Phytophtora sp. and other pathogens. Further results showed that the main factor of the side effects was caused by different qualities of plant material in interaction to the inoculation with the BCA and only to a minor extent depended on pre-infestation of soils. We conclude that specific conditions, such as certified plant material or soil analysis for other pathogens besides Verticillium, avoided these side-effects relevant for commercial farming.

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Influence of Soil Temperature to the Pathosystem Strawberry-Verticillium

Cited by 2 publications

References 0 publications

Aerial Warfare: A Volatile Dialogue between the Plant Pathogen Verticillium longisporum and Its Antagonist Paenibacillus polymyxa

Aerial Warfare: A Volatile Dialogue between the Plant Pathogen Verticillium longisporum and Its Antagonist Paenibacillus polymyxa

Field Application of Non-Pathogenic <i>Verticillium dahliae</i> Genotypes for Regulation of Wilt in Strawberry Plants

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Influence of Soil Temperature to the Pathosystem Strawberry-Verticillium

Cited by 2 publications

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Aerial Warfare: A Volatile Dialogue between the Plant Pathogen Verticillium longisporum and Its Antagonist Paenibacillus polymyxa

Aerial Warfare: A Volatile Dialogue between the Plant Pathogen Verticillium longisporum and Its Antagonist Paenibacillus polymyxa

Field Application of Non-Pathogenic &lt;i&gt;Verticillium dahliae&lt;/i&gt; Genotypes for Regulation of Wilt in Strawberry Plants

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Field Application of Non-Pathogenic <i>Verticillium dahliae</i> Genotypes for Regulation of Wilt in Strawberry Plants