Influence of sodium bicarbonate and standard antidotes on acid-base status in rats poisoned with dichlorvos

Stefanovic, Danka; Antonijević, Biljana; Bokonjić, Dubravko; Stojiljković, Miloš P.; Milovanović, Zoran; Nedeljković, M

doi:10.2298/jmh0402165s

Cited by 2 publications

(2 citation statements)

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“…It is, however, evident that the peripheral component of the toxicity of dichlorvos in our experiment was also important, since the bispyridinium oxime obidoxime, known for its limited passage through the blood‐brain barrier into the brain, also exerted an efficient protection against dichlorvos‐induced lethality in our previous and current experiments (Antonijevic et al 2002; Stefanovic et al 2004). These effects should be ascribed to their reactivating potencies, proven in several species poisoned with dichlorvos (Gyrd‐Hansen & Kraul 1984).…”

Section: Discussionmentioning

confidence: 52%

Effect of Sodium Bicarbonate in Rats Acutely Poisoned with Dichlorvos

Stefanovic

Antonijević

Bokonjić

et al. 2006

Basic Clin Pharma Tox

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The development of effective antidotes against organophosphates such as dichlorvos has been a persistent challenge over the past decades. Therapy of organophosphate poisoning is based on the administration of atropine and oxime as standard antidotes. The present study was undertaken to evaluate the ability of sodium bicarbonate to improve protective effects of standard antidotes in rats poisoned with dichlorvos. The aim of this experiment was to establish the correlation between protective effects and biochemical parameters relevant for acid-base status. In order to examine the protective effect of both standard antidotes and their combinations, groups of experimental animals were poisoned subcutaneously with increasing doses of dichlorvos. Immediately thereafter, rats were treated with atropine 10 mg/kg intramuscularly, oximes 10 mg/kg intramuscularly and sodium bicarbonate 3 mmol/kg intraperitoneally. These antidotes were administered either as single doses or in combinations. In the biochemical part of the experiments, rats were poisoned with dichlorvos 1.3 LD 50 (10.64 mg/kg) subcutaneously and immediately thereafter treated with atropine 10 mg/kg intramuscularly, oximes (trimedoxime or obidoxime) 10 mg/kg intramuscularly and sodium bicarbonate 3 mmol/kg intraperitoneally either as single doses or in combinations. Parameters relevant for acid-base status were measured 10 minutes after the administration of antidotes. The results of our study indicate that addition of sodium bicarbonate to standard antidotes significantly improves protective effects of atropine, obidoxime and trimedoxime. Correlation between protection and biochemical outcome is clearly evident when sodium bicarbonate is being added to atropine.

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Section: Discussionmentioning

confidence: 52%

Effect of Sodium Bicarbonate in Rats Acutely Poisoned with Dichlorvos

Stefanovic

Antonijević

Bokonjić

et al. 2006

Basic Clin Pharma Tox

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“…Experimental and clinical studies have shown a beneficial therapeutic effect of sodium bicarbonate when added to standard treatment for acute OP poisoning, but the mechanism is unclear (Balali-Mood et al, 2007;Stefanović et al, 2004Stefanović et al, , 2006Vučinić et al, 2007). Because sodium bicarbonate increases blood pH value, it is possible that a significant amount of OP is hydrolyzed at more alkaline pHs, improving clinical signs in patients poisoned with OP pesticides.…”

Section: Management Of Acute Poisoning With Op and CM Pesticidesmentioning

confidence: 99%