Influence of Smoking on Serum and Milk Malondialdehyde, Superoxide Dismutase, Glutathione Peroxidase, and Antioxidant Potential Levels in Mothers at the Postpartum Seventh Day

Abstract

The aim of the study was to investigate simultaneously serum and milk malondialdehyde (MDA) levels, superoxide dismutase (SOD), glutathione peroxidase (GPx) activities, and antioxidant potential (AOP) in active-smoking, passive-smoking, and nonsmoking mothers and to search if there is any difference between serum and milk oxidant/ antioxidant status caused by smoking. According to their smoking status, 60 mothers (age range: 20-35 yr) were classified into one of three groups: the active-smoking mothers (n=15), the passive-smoking mothers (n=22), and the nonsmoking mothers (n=23). Serum and milk MDA, SOD, GPx, and AOP values were determined in mothers on the postpartum seventh day by the spectrophotometric method. Serum Zn and Cu concentrations were determined by atomic absorption spectrophotometry (AAS). There was no significant difference in serum samples with respect to MDA (p=0.17), SOD (p=0.51), and AOP (p=0.36) levels, but there was a significant difference in serum GPx (p=0.002) levels among the study groups. The significant differences were also found in milk samples in terms of MDA (p=0.002) and SOD (p=0.011), but not in GPx (p=0.11) and AOP (p=0.29) levels among the study groups. No significant difference was seen in serum zinc concentration (p=0.49), but copper concentration differed significantly among the groups (p=0.005). These observations suggest that human milk is more vulnerable to oxidative stress and lipid peroxidation than serum samples in smoking mothers, even if they are passive smokers.

Effect of High Pressure and Sub-Zero Temperature on Total Antioxidant Capacity and the Content of Vitamin C, Fatty Acids and Secondary Products of Lipid Oxidation in Human Milk

“…The content of TBARS in human milk is determined by various factors, including the mother's health and exposure to cigarette smoke [Ermis et al, 2005]. In the tested samples of raw human milk, TBARS levels were determined at 3.35 μmol/L MDA, and similar results were reported by Turoli et al [2004].…”

Redox status, and lipid peroxidation and protein oxidation levels in small ruminants

“…TBARS were measured according to the method described by Ermis et al (2005). The product of lipid peroxidation was expressed in terms of malondialdehyde (MDA) equivalents (μM) using an external standard calibration curve, linear in the range between 0.125 and 8 μM, obtained from MDA generation with 1,1,3,3-tetraethoxypropane (TEP) hydrolysis.…”

Effects of plant extracts and essential oils as feed supplements on quality and microbial traits of rabbit meat

“…Products of lipid oxidation were measured in minced meat, quantitated using the thiobarbituric acid reactive substances (TBARS) method and are expressed as malondialdehyde (MDA) equivalents according to the method of Ermis et al (2005) with the following modifications. Briefly, 10 g of minced meat was homogenised with 10 mL of Phosphate Buffered Saline solution (PBS, Sigma-Aldrich, St-Louis, MO, USA).…”