Influence of smoking on methylation and hydroxymethylation levels in global DNA and specific sites of KRT14 , KRT19 , MIR-9-3 and MIR-137 genes of oral mucosa

Costa, Ludimila de Araújo; Silva, Isabelle Cristina Borba da; Mariz, Bruno Augusto Linhares Almeida; Silva, Mikaelly Batista da; Freitas-Ribeiro, Gláucia Marques; Oliveira, Naila Francis Paulo de

doi:10.1016/j.archoralbio.2016.08.013

Cited by 13 publications

(8 citation statements)

References 51 publications

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“…Multivariate linear regression models were used to examine the association between BPA exposure as categorized variables with hydroxymethylation of LINE-1. The following variables were adjusted as potential confounders: age, smoking, alcohol consumption and history of disease, which had been reported to be associated with DNA hydroxymethylation [35,36,37]. In addition, to examine a potential modifying effect on the association between BPA exposure and DNA hydroxymethylation of LINE-1, stratified analyses were conducted by age, smoking status, alcohol consumption and history of disease due to their known effects on 5hmC.…”

Section: Methodsmentioning

confidence: 99%

Association of Bisphenol A Exposure with LINE-1 Hydroxymethylation in Human Semen

Tian

Zhou

Miao

et al. 2018

IJERPH

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Bisphenol A (BPA), an exogenous endocrine-disrupting chemical, has been shown to alter DNA methylation. However, little information is available about the effect of BPA exposure on DNA hydroxymethylation in humans. The objective of the present study was to examine whether BPA exposure was associated with DNA hydroxymethylation in human semen samples. We measured urine BPA levels and LINE-1 hydroxymethylation in 158 male factory workers selected from an occupational cohort study conducted in China between 2004 and 2008. Among them, there were 72 male workers with occupational BPA exposure (BPA-exposed group) and 86 male workers without occupational BPA exposure (unexposed group). Multivariate linear regression models were used to examine the association of exposure to BPA with LINE-1 hydroxymethylation. LINE-1 was more highly hydroxymethylated in the BPA-exposed group than in the unexposed group (median 12.97% vs. 9.68%, respectively; p < 0.05), after adjusting for the potential confounders. The medians of 5-hydroxymethylcytosine (5hmC) generally increased with increasing urine BPA levels: 8.79%, 12.16%, 11.53%, and 13.45%, for undetected BPA and corresponding tertiles for the detected BPA, respectively. After analysis using data at individual level, our findings indicated that BPA exposure was associated with alterations of sperm LINE-1 hydroxymethylation, which might have implications for understanding the mechanisms underlying BPA-induced adverse effects on male reproductive function.

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Section: Methodsmentioning

confidence: 99%

Association of Bisphenol A Exposure with LINE-1 Hydroxymethylation in Human Semen

Tian

Zhou

Miao

et al. 2018

IJERPH

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“…The methylation profile is tissue-and site-specific, is associated with disease (Zhang, Lu, et al, 2020) and can be modulated by environmental factors such as UV radiation, nutrition, smoking, infection, physical activity, drugs, etc. (Corley et al, 2021;Costa et al, 2016;De Oliveira et al, 2020;Diniz et al, 2021;Esteller, 2017;Luna et al, 2018). It is important to mention that the VDR is expressed by oral cells (Ge et al, 2021), which makes the study of this gene in oral cells interesting, especially in the case of inflammation that affects the oral mucosa.…”

Section: Discussionmentioning

confidence: 99%

Polymorphism but not methylation status in the vitamin D receptor gene contributes to oral mucositis in children

et al. 2022

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ObjectiveTo investigate the relationship between the polymorphisms rs1544410 (BsmI), rs2228570 (FokI) and rs731236 (TaqI) and DNA methylation status in the VDR gene (vitamin D receptor) with oral mucositis (OM) in oncopaediatric patients treated with methotrexate (MTX®).MethodsThe population comprised healthy patients with haematological malignancies aged between 5 and 19 years. An evaluation of oral conditions was performed using the Oral Assessment Guide. Demographic, clinical, biochemical and haematological data were obtained from medical records. Genomic DNA from oral mucosal cells was used for the analysis of polymorphisms (n = 102) (PCR‐restriction fragment length polymorphism) and DNA methylation (n = 81) (methylation‐specific PCR).ResultsMales predominated (57.8%), and the mean age was 10.3 years (±4.7). OM affected 84.3% of patients, of which 53.1% developed severe oral mucositis (SOM). Patients with OM had lower platelet and leukocyte counts (p < 0.05). The G allele of rs1544410 (p = 0.040) and the CT genotype of rs2228570 polymorphisms were associated with SOM (p = 0.038). A partially methylated status in the VDR promoter was found in all patients.ConclusionOM is associated with lower leukocyte and platelet counts. SOM is associated with the rs1544410 and rs2228570 polymorphisms. The methylation status of the VDR is not associated with inflammation or exposure to MTX®.

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“…De Araújo Costa et al. 22 used mouthwash for collection of biological samples, which yielded heterogeneous cell populations from the oral cavity (i.e., lymphocytes and epithelial cells); in contrast, our method comprised collection of oral epithelial cells on cotton swabs. Furthermore, we used a fluorescence-based technique similar to that of enzyme-linked immunosorbent assays; our technique was more sensitive than the classical enzyme-linked immunosorbent assay method that measures absorbance values, which was used by De Araújo Costa et al.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, we used a fluorescence-based technique similar to that of enzyme-linked immunosorbent assays; our technique was more sensitive than the classical enzyme-linked immunosorbent assay method that measures absorbance values, which was used by De Araújo Costa et al. 22 …”

Section: Discussionmentioning

confidence: 99%

Changes in global DNA methylation and hydroxymethylation in oral mucosa according to tobacco smoke exposure

et al. 2020

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Objective This prospective clinical study comparatively investigated the effects of tobacco smoking on global methylation and hydroxymethylation in oral epithelial cells. Methods Buccal cells from the inside of the cheeks were collected from 47 individuals, including smokers, former smokers, and never smokers. DNA was extracted using dedicated kits. Methylated and hydroxymethylated DNA fractions were measured using assays similar to enzyme-linked immunosorbent assays. The levels of methylation and hydroxymethylation were compared among groups using unpaired two-tailed t-tests or the Mann–Whitney U test; P < 0.05 was considered statistically significant. Results There was no statistically significant difference in the average number of cigarettes between smoker and former smoker groups. Although methylation levels were lower for smokers (3.1%) and former smokers (2.16%), compared with never smokers (4.16%), these differences were not statistically significant. There was a two-fold increase in hydroxymethylation level in never smokers, compared with smokers. Conclusions Our findings suggest that smoking leads to global reductions in both methylation and hydroxymethylation levels in oral epithelial cells in a manner influenced by the intensity and length of exposure to tobacco smoke.

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Influence of smoking on methylation and hydroxymethylation levels in global DNA and specific sites of KRT14 , KRT19 , MIR-9-3 and MIR-137 genes of oral mucosa

Cited by 13 publications

References 51 publications

Association of Bisphenol A Exposure with LINE-1 Hydroxymethylation in Human Semen

Association of Bisphenol A Exposure with LINE-1 Hydroxymethylation in Human Semen

Polymorphism but not methylation status in the vitamin D receptor gene contributes to oral mucositis in children

Changes in global DNA methylation and hydroxymethylation in oral mucosa according to tobacco smoke exposure

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