“…Apart from this, N-and C-termini of the intact polypeptide chain (prepro-GGT) of bacterial GGTs were also studied for their importance in autoprocessing. Truncation in the N-terminal region, including the signal peptide, of EcGGT and BlGGT demonstrated that signal peptide is critical for proper enzyme folding and processing (Lin et al, 2008;Lo et al, 2008). For EcGGT, truncation beyond 17 amino acids was found detrimental for maturation, indicating the crucial role of the last nine amino acids of the signal peptide in functional expression of the enzyme (Lo et al, 2008), while in BlGGT, all the truncated mutants except the native enzyme resulted in partial or complete loss of processing, emphasizing again on the importance of signal peptide in the enzyme's folding and maturation (Lin et al, 2008).…”