Influence of short-term constant light on phase shift of mouse circadian locomotor activity rhythm induced by agonist and antagonist of serotonin

Bartoszewicz, Renata; Chmielewska, Daria; Domon, Magdalena M.; Barbacka-Surowiak, G

doi:10.1080/09291010903018016

Cited by 3 publications

(2 citation statements)

References 40 publications

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“…Our findings revealed a significant decline in activity under LD22 conditions, while alcohol access did not affect the levels of wheel running. Prior studies have reported conflicting effects of non-standard LD conditions on locomotion, with both increases and decreases observed in male rodents (Bartoszewicz et al, 2009; Delorme et al, 2022; Okuliarova et al, 2016). In female mice, both forward and inverted (shift work-like) photoperiod shifts were found to decrease locomotion (Banks et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Effects of chronodisruption and alcohol consumption on gene expression in reward-related brain areas in female rats

Meyer,

Schoettner,

Amir

2024

Preprint

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Circadian dysfunction caused by exposure to aberrant light-dark conditions is associated with abnormal alcohol consumption in humans and animal models. Changes in drinking behavior have been linked to alterations in clock gene expression in reward-related brain areas, which could be attributed to either the effect of chronodisruption or alcohol. To date, however, the combinatory effect of circadian disruption and alcohol on brain functions is less understood. Moreover, despite known sex differences in alcohol drinking behavior, most research has been carried out on male subjects only, and therefore implications for females remain unclear.To address this gap, adult female rats housed under an 11h/11h light-dark cycle (LD22) or standard light conditions (LD24, 12h/ 12h light-dark) were given access to an intermittent alcohol drinking protocol (IA20%) to assess the impact on gene expression in brain areas implicated in alcohol consumption and reward: the prefrontal cortex (PFC), nucleus accumbens (NAc), and dorsal striatum (DS). mRNA expression of core clock genes (Bmal1,Clock,Per2), sex hormone receptors (ERβ,PR), glutamate receptors (mGluR5,GluN2B), a calcium-activated channel (Kcnn2), and an inflammatory cytokine (TNF-α) were measured at two-time points relative to the locomotor activity cycle.Housing under LD22 did not affect alcohol intake but significantly disrupted circadian activity rhythms and reduced locomotion. Significant changes in the expression ofBmal1,ERβ, andTNF-αwere primarily related to the aberrant light conditions, whereas changes inPer2andPRexpression were associated with the effect of alcohol. Collectively, these results indicate that disruption of circadian rhythms and/or intermittent alcohol exposure have distinct effects on gene expression in the female brain, which may have implications for the regulation of alcohol drinking, addiction, and, ultimately, brain health.

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Section: Discussionmentioning

confidence: 99%

Effects of chronodisruption and alcohol consumption on gene expression in reward-related brain areas in female rats

Meyer,

Schoettner,

Amir

2024

Preprint

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“…ChronoPhysiology and Therapy 2013:3 serotonergic potentiation 8 of light-induced phase shifts. Therefore, even after taking into consideration the fact that potentiation responses are generally opposite in nocturnal and diurnal mammals, 9 usage of 5-HTP-containing drinks at user-selected times, or without knowledge of previous history of circadian entrainment and disruption, can potentially lead to resetting of the biological rhythm in unwanted ways.…”

Section: Publish Your Work In This Journalmentioning

confidence: 99%

The 5-HTP sip tryp: a timely word to the wise

Basu¹,

Singaravel²

2013

CPT

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Recently, an editorial in Nature Neuroscience pointed out the need for exercising caution in the use of over-the-counter drinks purporting to promote relaxation and containing, among other substances with chronobiotic activity, L-5-Hydroxytryptophan (5-HTP).1 This reality-check certainly raises an issue worth the attention of scientists who deal with, and laymen who dabble in, biological rhythms. Proprietary and patented formulations often project an image of safety, which is augmented by their over-thecounter availability as attractively packaged nutritional supplements. The allure of these bottled shots of bliss may seem undeniable to the general public. An online search of the words "5-HTP relaxation drink" returns . 7 million websites. Websites promoting these drinks do carry limited warnings or disclaimers (in fine print). The actual concentration of constituents is sometimes not provided in the online information leaflets. Certain websites provide a Natural Standard Editorial Board blinded review, 2,3 which summarizes known scientific literature (exhaustively) and mentions likely safe doses. In view of the rising popularity and wide availability of 5-HTP containing drinks, we feel that scientists and the general public alike would profit from an additional nugget of information regarding a previously unforeseen effect of 5-HTP consumption at an inappropriate time, as brought to light by recent literature in the area. 5-HTP crosses the blood -brain barrier upon systemic administration, bypasses the rate-limiting step in serotonin synthesis, elevates brain serotonin, 4 and affects non-REM sleep 5 in rodents, generally promoting wakefulness if administered at light onset, and promoting non-REM sleep with dark onset administration. In a recent study (March 2012), we investigated some of the effects 5-HTP had on the mammalian circadian system, using a nocturnal model. 6 In nocturnal mice, 5-HTP causes phase advances during the mid-subjective day, phase delays during the mid-subjective night and, at certain other times, ambiguous or no apparent phase-shifting effects at all. In this paper, we had expressed concern regarding the potentially detrimental effects caused by temporally inappropriate administration of 5-HTP, especially in cases where the circadian system has been previously subjected to a stressor, for example jet lag, and this is a caveat not mentioned by many, or perhaps any, marketers of these products. Additionally, experiments conducted at our lab using nocturnal field mice 7 indicate that 5-HTP administered in the late subjective night, when combined with light, may actually transiently increase locomotor activity, along with increasing light-induced phase shifts, through possibly arousal-dependent mechanisms most likely involving Dovepress submit your manuscript | www.dovepress.com

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l-5-hydroxytryptophan resets the circadian locomotor activity rhythm of the nocturnal Indian pygmy field mouse, Mus terricolor

Basu

Singaravel

Haldar

2012

Naturwissenschaften

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We report that L-5-hydroxytryptophan (5-HTP), a serotonin precursor, resets the overt circadian rhythm in the Indian pygmy field mouse, Mus terricolor, in a phase- and dose-dependent manner. We used wheel running to assess phase shifts in the free-running locomotor activity rhythm. Following entrainment to a 12:12 h light-dark cycle, 5-HTP (100 mg/kg in saline) was intraperitoneally administered in complete darkness at circadian time (CT)s 0, 3, 6, 9, 12, 15, 18, and 21, and the ensuing phase shifts in the locomotor activity rhythm were calculated. The results show that 5-HTP differentially shifts the phase of the rhythm, causing phase advances from CT 0 to CT 12 and phase delays from CT 12 to CT 21. Maximum advance phase shift was at CT 6 (1.18 ± 0.37 h) and maximum delay was at CT 18 (-2.36 ± 0.56 h). No extended dead zone is apparent. Vehicle (saline) at any CT did not evoke a significant phase shift. Investigations with different doses (10, 50, 100, and 200 mg/kg) of 5-HTP revealed that the phase resetting effect is dose-dependent. The shape of the phase-response curve (PRC) has a strong similarity to PRCs obtained using some serotonergic agents. There was no significant increase in wheel-running activity after 5-HTP injection, ruling out behavioral arousal-dependent shifts. This suggests that this phase resetting does not completely depend on feedback of the overt rhythmic behavior on the circadian clock. A mechanistic explanation of these shifts is currently lacking.

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Influence of short-term constant light on phase shift of mouse circadian locomotor activity rhythm induced by agonist and antagonist of serotonin

Cited by 3 publications

References 40 publications

Effects of chronodisruption and alcohol consumption on gene expression in reward-related brain areas in female rats

Effects of chronodisruption and alcohol consumption on gene expression in reward-related brain areas in female rats

The 5-HTP sip tryp: a timely word to the wise

l-5-hydroxytryptophan resets the circadian locomotor activity rhythm of the nocturnal Indian pygmy field mouse, Mus terricolor

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