Influence of shape and dispersion media of titanium dioxide nanostructures on microvessel network and ossification

Freyre-Fonseca, Verónica; Medina‐Reyes, Estefany I.; Téllez‐Medina, Darío Iker; Paniagua-Contreras, Gloria Luz; Monroy-Pérez, Eric; Vaca-Paniagua, Felipe; Delgado-Buenrostro, Norma L.; Flores-Flores, José O.; López-Villegas, Edgar Óliver; Gutiérrez-López, Gustavo F.; Chirino, Yolanda I.

doi:10.1016/j.colsurfb.2017.11.049

Cited by 12 publications

(16 citation statements)

References 38 publications

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“…There is minimal data available regarding bone implants on CAM [23,24]. Despite this, there is some testing of the vessel acquisition ability of different implanted biomaterials, such as 3D printed hydroxyapatite scaffolds [25] or nanoparticles [26]. CAM is even less used as an experimental model in dental medicine research.…”

Section: Introductionmentioning

confidence: 99%

Hyaluronic Acid/Bone Substitute Complex Implanted on Chick Embryo Chorioallantoic Membrane Induces Osteoblastic Differentiation and Angiogenesis, but not Inflammation

Cirligeriu

Cîmpean

Călniceanu

et al. 2018

IJMS

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Microscopic and molecular events related to alveolar ridge augmentation are less known because of the lack of experimental models and limited molecular markers used to evaluate this process. We propose here the chick embryo chorioallantoic membrane (CAM) as an in vivo model to study the interaction between CAM and bone substitutes (B) combined with hyaluronic acid (BH), saline solution (BHS and BS, respectively), or both, aiming to point out the microscopic and molecular events assessed by Runt-related transcription factor 2 (RUNX 2), osteonectin (SPARC), and Bone Morphogenic Protein 4 (BMP4). The BH complex induced osteoprogenitor and osteoblastic differentiation of CAM mesenchymal cells, certified by the RUNX2 +, BMP4 +, and SPARC + phenotypes capable of bone matrix synthesis and mineralization. A strong angiogenic response without inflammation was detected on microscopic specimens of the BH combination compared with an inflammatory induced angiogenesis for the BS and BHS combinations. A multilayered organization of the BH complex grafted on CAM was detected with a differential expression of RUNX2, BMP4, and SPARC. The BH complex induced CAM mesenchymal cells differentiation through osteoblastic lineage with a sustained angiogenic response not related with inflammation. Thus, bone granules resuspended in hyaluronic acid seem to be the best combination for a proper non-inflammatory response in alveolar ridge augmentation. The CAM model allows us to assess the early events of the bone substitutes–mesenchymal cells interaction related to osteoblastic differentiation, an important step in alveolar ridge augmentation.

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Section: Introductionmentioning

confidence: 99%

Hyaluronic Acid/Bone Substitute Complex Implanted on Chick Embryo Chorioallantoic Membrane Induces Osteoblastic Differentiation and Angiogenesis, but not Inflammation

Cirligeriu

Cîmpean

Călniceanu

et al. 2018

IJMS

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“…Free access to the CAM allows a variety of analytical methods (Figure 1) [57]. While some researchers evaluate chemical irritation of a specific substance on the CAM [16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31], further investigation such as vascularization or developmental changes can also be addressed [32][33][34][35][36][37][38][39][40][41][42][43][44][45].…”

Section: Analytical Methodologies Provided By the Cam Assaymentioning

confidence: 99%

“…Since the opening of the eggshell allows direct observation of the CAM optical evaluation by microscopy [29,[31][32][33]36,37,[42][43][44][45][46][47][48][49][50]52] as well as imaging techniques such as ultrasonography [85], computed tomography [86], and magnetic resonance tomography [87] have been successfully used in the CAM assay (Figure 1).…”

Section: Analytical Methodologies Provided By the Cam Assaymentioning

confidence: 99%

“…As the CAM has a dense vascular network extending during development, the impact of nanoparticles on angiogenesis and vasculogenesis can be observed in detail [58]. Yet, several authors have evaluated the influence of different nanoparticulate substances on angiogenesis [32][33][34][35][36][37][38][39][40][41][42][43][44][45]. The number of vessel branches as well as vessel size and vascular density are frequently used as parameters for quantification [34,37,40,41,[43][44][45].…”

Section: Methods To Assess Vascularizationmentioning

confidence: 99%

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The Chorioallantoic Membrane Assay in Nanotoxicological Research—An Alternative for In Vivo Experimentation

et al. 2020

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Nanomaterials unveil many applicational possibilities for technical and medical purposes, which range from imaging techniques to the use as drug carriers. Prior to any human application, analysis of undesired effects and characterization of their toxicological profile is mandatory. To address this topic, animal models, and rodent models in particular, are most frequently used. However, as the reproducibility and transferability to the human organism of animal experimental data is increasingly questioned and the awareness of animal welfare in society increases at the same time, methodological alternatives are urgently required. The chorioallantoic membrane (CAM) assay is an increasingly popular in ovo experimental organism suitable for replacement of rodent experimentation. In this review, we outline several application fields for the CAM assay in the field of nanotoxicology. Furthermore, analytical methods applicable with this model were evaluated in detail. We further discuss ethical, financial, and bureaucratic aspects and benchmark the assay with other established in vivo models such as rodents.

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“…The potential hazard of NPs depends on their intrinsic physico-chemical properties (composition, size, concentration and shape), their surface modifications and their interaction with the environment [5][6][7][8][9][10][11]. The latter results in the formation of a biocorona created by complex matrices (e.g., lipids in cosmetics, proteins in cell culture media or bio-fluids) and influence the uptake, dissolution or aggregation behavior of NPs in biological systems [12].…”

Section: Introductionmentioning

confidence: 99%

Tackling Complex Analytical Tasks: An ISO/TS-Based Validation Approach for Hydrodynamic Chromatography Single Particle Inductively Coupled Plasma Mass Spectrometry

et al. 2020

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Nano-carrier systems such as liposomes have promising biomedical applications. Nevertheless, characterization of these complex samples is a challenging analytical task. In this study a coupled hydrodynamic chromatography-single particle-inductively coupled plasma mass spectrometry (HDC-spICP-MS) approach was validated based on the technical specification (TS) 19590:2017 of the international organization for standardization (ISO). The TS has been adapted to the hyphenated setup. The quality criteria (QC), e.g., linearity of the calibration, transport efficiency, were investigated. Furthermore, a cross calibration of the particle size was performed with values from dynamic light scattering (DLS) and transmission electron microscopy (TEM). Due to an additional Y-piece, an online-calibration routine was implemented. This approach allows the calibration of the ICP-MS during the dead time of the chromatography run, to reduce the required time and enhance the robustness of the results. The optimized method was tested with different gold nanoparticle (Au-NP) mixtures to investigate the characterization properties of HDC separations for samples with increasing complexity. Additionally, the technique was successfully applied to simultaneously determine both the hydrodynamic radius and the Au-NP content in liposomes. With the established hyphenated setup, it was possible to distinguish between different subpopulations with various NP loads and different hydrodynamic diameters inside the liposome carriers.

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Influence of shape and dispersion media of titanium dioxide nanostructures on microvessel network and ossification

Cited by 12 publications

References 38 publications

Hyaluronic Acid/Bone Substitute Complex Implanted on Chick Embryo Chorioallantoic Membrane Induces Osteoblastic Differentiation and Angiogenesis, but not Inflammation

Hyaluronic Acid/Bone Substitute Complex Implanted on Chick Embryo Chorioallantoic Membrane Induces Osteoblastic Differentiation and Angiogenesis, but not Inflammation

The Chorioallantoic Membrane Assay in Nanotoxicological Research—An Alternative for In Vivo Experimentation

Tackling Complex Analytical Tasks: An ISO/TS-Based Validation Approach for Hydrodynamic Chromatography Single Particle Inductively Coupled Plasma Mass Spectrometry

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