Influence of sex on gene expression in the mouse lacrimal gland

Richards, Stephen M.; Jensen, Roderick V.; Lv, Meng; Sullivan, Benjamin D.; Lombardi, Michael J.; Rowley, Patricia; Schirra, F.; Treister, Nathaniel S.; Suzuki, Tomo; Steagall, Rebecca J.; Yamagami, Hiroko; Sullivan, David A.

doi:10.1016/j.exer.2005.04.014

Cited by 37 publications

(49 citation statements)

References 35 publications

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“…Our findings are consistent with the gender associated and gland specific variations in mRNA levels previously reported in rodent model salivary gland systems (20,27,28) and in lacrimal (29) and meibomian glands (30). The differentially expressed genes in these studies are involved in a wide range of biological processes, molecular functions and cellular components, including growth and development, transcription, metabolism, signal transduction, ion transport, receptor activity and protein and nucleic acid binding.…”

Section: Discussionsupporting

confidence: 79%

Age and gender related differences in human parotid gland gene expression

Srivastava

Wang

Zhou

et al. 2008

Archives of Oral Biology

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Objective-The present study evaluated differences in gene expression associated with age and gender in the human parotid gland.Design-Parotid gland tissue was analyzed using the Affymetrix® GeneChip® HGU133plus2.0 array.Results-Differential gene expression, defined as a statistically significant difference with a 1.5 fold or greater change, was detected in 787 gene probe sets; 467 (~59%) showed higher expression in females. Several genes associated with saliva secretion were differentially expressed in male and female parotid glands including vesicle-associated membrane protein 3 VAMP3, synaptosomalassociated protein SNAP23, RAS oncogene family member RAB1A and the syntaxin binding protein STXBP1. Evaluation of gene expression in the youngest and the oldest female subjects revealed that the expression of 228 probe sets were altered during aging; 155 genes were up-regulated in the aged female parotid gland. However, of the genes that were altered during aging, 22 of the 30 probes (73%) classified as being associated with immune responses were down-regulated in the aged parotid gland. A panel of differentially expressed, age-and gender-related genes was selected for validation by quantitative, real-time RT-PCR. Comparable differences in gene expression were detected by both Affymetrix array and quantitative, real-time RT-PCR methods.Conclusions-Our data suggest that salivary gland function may be adversely affected in the aged population due, at least in part, to the altered regulation of several categories of genes. Moreover, the gender specific differences in gene expression identified in the present study correlate with the previously observed sexual dimorphism in salivary gland function.

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Section: Discussionsupporting

confidence: 79%

Age and gender related differences in human parotid gland gene expression

Srivastava

Wang

Zhou

et al. 2008

Archives of Oral Biology

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“…It is clear that the regulatory pathways underlying sexual differentiation result in extensive differences in gene expression in adults. Briefly, tens to thousands of genes were shown to be sexually dimorphic in mouse liver, kidney, blastocysts, lacrimal gland, prenatal brain, and adult brain substructures (Lauber et al 1991;Anderson 2002;Xu et al 2005;Dewing et al 2003;Rinn et al 2004;Vawter et al 2004;AmadorNoguez et al 2005;Clodfelter et al 2006;Kobayashi et al 2006;Richards et al 2006). However, because of the limited power resulting from relatively small sample sizes in most studies, the extent and tissue specificity of sexual dimorphism in gene expression have not been fully explored.…”

mentioning

confidence: 99%

Tissue-specific expression and regulation of sexually dimorphic genes in mice

Yang¹,

Schadt²,

Wang³

et al. 2006

Genome Res.

785

724

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We report a comprehensive analysis of gene expression differences between sexes in multiple somatic tissues of 334 mice derived from an intercross between inbred mouse strains C57BL/6J and C3H/HeJ. The analysis of a large number of individuals provided the power to detect relatively small differences in expression between sexes, and the use of an intercross allowed analysis of the genetic control of sexually dimorphic gene expression. Microarray analysis of 23,574 transcripts revealed that the extent of sexual dimorphism in gene expression was much greater than previously recognized. Thus, thousands of genes showed sexual dimorphism in liver, adipose, and muscle, and hundreds of genes were sexually dimorphic in brain. These genes exhibited highly tissue-specific patterns of expression and were enriched for distinct pathways represented in the Gene Ontology database. They also showed evidence of chromosomal enrichment, not only on the sex chromosomes, but also on several autosomes. Genetic analyses provided evidence of the global regulation of subsets of the sexually dimorphic genes, as the transcript levels of a large number of these genes were controlled by several expression quantitative trait loci (eQTL) hotspots that exhibited tissue-specific control. Moreover, many tissue-specific transcription factor binding sites were found to be enriched in the sexually dimorphic genes.

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“…Towards this end, glandular RNA samples were purified further with RNAqueous spin columns (Ambion, Austin, TX), and the integrity of these preparations was determined with a RNA 6000 Nano LabChip with an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA). The RNA samples were then processed for CodeLink Bioarray hybridization, as previously reported [15]. In brief, cDNA was synthesized from RNA (2 g) with a CodeLink Expression Assay Reagent Kit (Amersham, Piscataway, NJ) and isolated with a QIAquick purification kit (Qiagen, Valencia, CA).…”

Section: Molecular Biological Proceduresmentioning

confidence: 99%

“…Sense and anti-sense primers were designed, as previously reported [15], by using Primer Express Software, version 1.5a (Applied Biosystems Inc., Foster City, CA). Real-time PCR reactions were carried out according to the manufacturer's specifications, by using aliquots of lacrimal gland cDNA (0.01-0.3 l cDNA), optimal primer concentrations, and Applied Biosystems' SYBR Green PCR Master Mix, MicroAmp Optical 96-Well Reaction Plates, ABI PRISM Optical Adhesive Covers and the GeneAmp 7900 HT Sequence Detection System.…”

Section: Real-time Pcr Proceduresmentioning

confidence: 99%