Influence of serum and polystyrene plate type on stability of Candida albicans biofilms

Kipanga, Purity N.; Luyten, Walter

doi:10.1016/j.mimet.2017.04.006

Cited by 14 publications

(16 citation statements)

References 43 publications

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“…In this study, we used C. albicans SC5314 strain, and the MIC was found in the range of 6.25–12.5 µ M. C. albicans 5314 was used because the genome is fully described, with well-known molecular patterns and phenotypes. In addition, the biofilm formation by this strain is well established in several studies [ 22 , 23 ].…”

Section: Discussionmentioning

confidence: 99%

Fungal-Host Interaction: Curcumin Modulates Proteolytic Enzyme Activity ofCandida albicansand Inflammatory Host ResponseIn Vitro

Chen

Benso

Seleem

et al. 2018

International Journal of Dentistry

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Current treatments for Candida albicans infection are limited due to the limited number of antifungal drugs available and the increase in antifungal resistance. Curcumin is used as a spice, food preservative, flavoring, and coloring agent that has been shown to have many pharmacological activities. Thus, this study evaluated the modulatory effects of curcumin on major virulence factors associated with the pathogenicity of C. albicans. The minimum inhibitory concentration (MIC) of curcumin against C. albicans (SC5314) was determined. Biofilm formation was quantified and the proteinase and phospholipase secretion was measured. The cytotoxicity was tested in oral fibroblast cells. A cocultured model was used to analyze the gene expression of proinflammatory cytokines (IL-1β, IL-1α, and IL-6) from host cells, as well SAP-1 and PLB-1 by RT-PCR. The MIC was between 6.25 and 12.5 µM, and the activity of proteinase enzyme was significantly decreased in biofilms treated with curcumin. However, proteinase gene expression was not downregulated after curcumin treatment. Furthermore, gene expressions of host inflammatory response, IL-1β and IL-1α, were significantly downregulated after exposure to curcumin. In conclusion, curcumin exhibited antifungal activity against C. albicans and modulated the proteolytic enzyme activities without downregulating the gene expression. In host inflammatory response, curcumin downregulated IL-1β and IL-1α gene expression.

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Section: Discussionmentioning

confidence: 99%

Fungal-Host Interaction: Curcumin Modulates Proteolytic Enzyme Activity ofCandida albicansand Inflammatory Host ResponseIn Vitro

Chen

Benso

Seleem

et al. 2018

International Journal of Dentistry

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“…Similarly, Frade et al [25] found that serum enhanced biofilm productivity of Candida albicans on metallic and non-metallic surfaces. Finally, using methodologies most similar to our approach, Kipanga et al [26] demonstrated that polystyrene microtiter plates…”

Section: Protein As a Surface Conditioning Agentmentioning

confidence: 91%

Exogenous Protein as an Environmental Stimulus of Biofilm Formation in Select Bacterial Strains

Ye¹,

Bapu²,

Cavalcante³

et al. 2020

AiM

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A screening of environmental conditions that would elicit robust biofilm in a collection of Serratia marcescens isolated from soil revealed that exogenous milk protein increased biofilm productivity up to tenfold. A select screening of fish pathogens, freshwater and human isolates identified several other species that responded similarly to exogenous protein. The optimal protein concentration was species specific; S. marcescens at 5% milk protein, Aeromonas sp. at 2%-3%, Flavobacterium columnare at 1% and Pseudomonas aeruginosa at 0.1%-0.4%. Media supplemented with milk protein also increased the cell counts in biofilm as well as the protein incorporated into the biofilm matrix. These data suggest that relatively high concentrations of exogenous protein may serve as an environmental trigger for biofilm formation, particularly for pathogenic bacteria exposed to relatively high concentrations of protein in bodily fluids and mucosal surfaces.

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“…Similarly, Frade et al (25) found that serum enhanced biofilm productivity of Candida albicans on metallic and non-metallic surfaces. Finally, using methodologies most similar to our approach, Kipanga et al (26) demonstrated that polystyrene microtiter plates (Costar) conditioned with foetal calf serum showed reduced biofilm formation by C. albicans . These assays are in general difficult to compare given the diversity of surfaces, strains and complexity of serum.…”

Section: Discussionmentioning

confidence: 79%

Exogenous Protein as an Environmental Stimuli of Biofilm Formation in Select Bacterial Strains

Bapu

Cavalcante

et al. 2019

Preprint

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A screening of environmental conditions that would elicit robust biofilm in a collection of 17Serratia marcescens isolated from soil revealed that exogenous milk protein increased biofilm 18 productivity up to ten-fold. A select screening of fish pathogens, freshwater and human isolates 19identified several other species that responded similarly to exogenous protein. The optimal protein 20 concentration was species specific; S. marcescens at 5% milk protein, Aeromonas sp. at 2-3%, 21Flavobacterium columnare at 1% and Pseudomonas aeruginosa at 0.1-0.4%. Media supplemented 22

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Influence of serum and polystyrene plate type on stability of Candida albicans biofilms

Cited by 14 publications

References 43 publications

Fungal-Host Interaction: Curcumin Modulates Proteolytic Enzyme Activity ofCandida albicansand Inflammatory Host ResponseIn Vitro

Fungal-Host Interaction: Curcumin Modulates Proteolytic Enzyme Activity ofCandida albicansand Inflammatory Host ResponseIn Vitro

Exogenous Protein as an Environmental Stimulus of Biofilm Formation in Select Bacterial Strains

Exogenous Protein as an Environmental Stimuli of Biofilm Formation in Select Bacterial Strains

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