Influence of semiflexible structural features of actin cytoskeleton on cell stiffness based on actin microstructural modeling

Wang, Kaiqun; Sun, Dong

doi:10.1016/j.jbiomech.2012.05.030

Cited by 32 publications

(26 citation statements)

References 42 publications

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“…Compared with the nuclear regions, cell stiffnesses in nonnuclear areas fall under a lower and broader range (,0.2-3 kPa), which is largely governed by F-actin density, (Pesen and Hoh, 2005;Rotsch and Radmacher, 2000;Wakatsuki et al, 2001;Wang and Sun, 2012). To test the hypothesis that diapedesis sites might correlate inversely with local F-actin density and stiffness (which varies greatly within individual cells), we first carried out dynamic imaging to assess the density of actin-GFP at sites where T cells transmigrated, relative to the averaged actin-GFP density across the entire cell area.…”

Section: Ilps Might Function In Local Sampling Of Endothelial Stiffnessmentioning

confidence: 99%

Probing the biomechanical contribution of the endothelium to lymphocyte migration: diapedesis by the path of least resistance

Martinelli

Zeiger

Whitfield

et al. 2014

Journal of Cell Science

104

148

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Immune cell trafficking requires the frequent breaching of the endothelial barrier either directly through individual cells ('transcellular' route) or through the inter-endothelial junctions ('paracellular' route). What determines the loci or route of breaching events is an open question with important implications for overall barrier regulation. We hypothesized that basic biomechanical properties of the endothelium might serve as crucial determinants of this process. By altering junctional integrity, cytoskeletal morphology and, consequently, local endothelial cell stiffness of different vascular beds, we could modify the preferred route of diapedesis. In particular, high barrier function was associated with predominantly transcellular migration, whereas negative modulation of junctional integrity resulted in a switch to paracellular diapedesis. Furthermore, we showed that lymphocytes dynamically probe the underlying endothelium by extending invadosome-like protrusions (ILPs) into its surface that deform the nuclear lamina, distort actin filaments and ultimately breach the barrier. Fluorescence imaging and pharmacologic depletion of F-actin demonstrated that lymphocyte barrier breaching efficiency was inversely correlated with local endothelial F-actin density and stiffness. Taken together, these data support the hypothesis that lymphocytes are guided by the mechanical 'path of least resistance' as they transverse the endothelium, a process we term 'tenertaxis'.

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Section: Ilps Might Function In Local Sampling Of Endothelial Stiffnessmentioning

confidence: 99%

Probing the biomechanical contribution of the endothelium to lymphocyte migration: diapedesis by the path of least resistance

Martinelli

Zeiger

Whitfield

et al. 2014

Journal of Cell Science

104

148

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“…Cytoskeleton rearrangements has been shown to alter the viscoelastic responses of ECs. 37 As reported, higher concentration of stress fibers contributes ECs stiffness. 28 Moreover, the dense peripheral band may be contractile.…”

Section: Discussionmentioning

confidence: 77%

LDL Decreases the Membrane Compliance and Cell Adhesion of Endothelial Cells Under Fluid Shear Stress

et al. 2012

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Atherosclerosis is an inflammatory disease of large and medium sized arteriole walls that is precipitated by elevated levels of low-density lipoprotein (LDL) cholesterol in the blood. However, the mechanisms that lead to the initiation of atherosclerosis are not fully understood. In this study, endothelial cells (ECs) were incubated with LDL for 24 h, and then the lipid was detected with Oil Red O staining and cholesterol ester was assayed with high-performance liquid chromatography (HPLC). F-actin was examined by fluorescence microscopy and the viscoelasticity of ECs was investigated using the micropipette aspiration technique. Then, a parallel-plate flow chamber device was used to observe the adhesion and retention of ECs under shear stress. The results demonstrated that elevated LDL significantly increased the cellular lipid content and induced the rearrangement of cytoskeletal F-actin. The initial rapid deformability (l/K 1 + l/K 2) was reduced by elevated cellular LDL levels, while membrane viscosity (μ) was increased by LDL accumulation. After treatment with 150 mg L(-1) LDL for 24 h, the adhesion of ECs under fluid shear stress was significantly decreased (p < 0.05). These results suggested that LDL induced cellular lipid accumulation and cytoskeleton reorganization which increased the cellular stiffness and decreased the adhesion of ECs.

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“…Cell stiffness value closely relates to the structure of the cell's membrane and the cytoskeleton within the cell. Therefore, the stiffness of a single cell reflects the cell function in many aspects [57,58]. For example, the stiffness of the mammalian cell shows obvious difference after medical treatment or after death, which could benefit disease diagnosis, drug development, and disease therapy [59][60][61].…”

Section: Cell Characterizationmentioning

confidence: 99%

State of the art: micro-nanorobotic manipulation in single cell analysis

Shen

Fukuda

2014

Robot. Biomim.

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Single cell analysis is an essential research approach to reveal the secret of life. At such a small scale, it puts forward higher demand on the accuracy of the cell manipulation system. This paper reviews the state of the art of micro-nanorobotic manipulation in single cell analysis. First, the key applications of the micro-nanorobotic manipulation system in single cell analysis are introduced, including the single cell injection, positioning, characterization, assembly, and the development of biomedical device. Then, the current key techniques, challenges, and future trends in micro-nanorobot are discussed from the aspects of actuating, sensing, controlling, system integration, and commercialization. To meet the requirement of cell biology, the next generation micro-nanorobot should have small, automatic, high integration and applicable features in multiscale.

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Influence of semiflexible structural features of actin cytoskeleton on cell stiffness based on actin microstructural modeling

Cited by 32 publications

References 42 publications

Probing the biomechanical contribution of the endothelium to lymphocyte migration: diapedesis by the path of least resistance

Probing the biomechanical contribution of the endothelium to lymphocyte migration: diapedesis by the path of least resistance

LDL Decreases the Membrane Compliance and Cell Adhesion of Endothelial Cells Under Fluid Shear Stress

State of the art: micro-nanorobotic manipulation in single cell analysis

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