Influence of semen processing technique on human sperm DNA integrity

Zini, Armand; Finelli, Anthony; Phang, Donna; Jarvi, Keith

doi:10.1016/s0090-4295(00)00770-6

Cited by 179 publications

(137 citation statements)

References 19 publications

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“…Zini et al, however, demonstrated a significant increase in sperm with undamaged DNA after seminal processing performed by swim-up. This increase was not observed when density gradient discontinous centrifugation was performed in the same samples [39]. A study by our group also demonstrated that semen processing by a discontinuous density gradient did not improve apoptotic SD standard deviation, 95% CI 95% confidence interval of the mean *p<α DNA fragmentation, evaluated by the TUNEL technique [40].…”

Section: Discussionmentioning

confidence: 84%

Effect of leukocytospermia and processing by discontinuous density gradient on sperm nuclear DNA fragmentation and mitochondrial activity

Fariello

Giudice

Spaine

et al. 2009

J Assist Reprod Genet

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Purpose To assess the effect of leukocytospermia and semen processing on sperm DNA and mitochondria. Methods Twenty-two patients with and 41 without leukocytospermia were included. Sperm DNA fragmentation was assessed by the Comet assay, and mitochondrial activity by a colorimetric method for active mitochondria. Semen was processed using Percoll, and motility, DNA fragmentation, and mitochondrial activity were analyzed pre-and post-processing. Results No differences were observed in age, abstinence, volume, sperm morphology, progressive motility, concentration, and vitality (p>0.10). Variables were grouped according to time (pre-vs post-processing) and group (leukocytospermia vs non-leukocytospermia) because no interactions could be observed. Leukocytospermia was associated to increased DNA fragmentation, while semen processing led to a decrease in DNA fragmentation and to increased mitochondrial activity. Conclusion While semen processing selects sperm with higher rates of DNA integrity independent of the presence or absence of leukocytes in semen, samples without leukocytospermia present more sperm without DNA fragmentation. Semen processing also selects sperm with higher mitochondrial activity.

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Section: Discussionmentioning

confidence: 84%

Effect of leukocytospermia and processing by discontinuous density gradient on sperm nuclear DNA fragmentation and mitochondrial activity

Fariello

Giudice

Spaine

et al. 2009

J Assist Reprod Genet

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“…While one study showed that the DGC is more effective than SUP in reducing the percentage of sperm with DNA damage [25], another one, in contrast, demonstrated that SUP recovers lower percentage of sperm with fragmented DNA as compared to DGC [37]. Enciso et al [11] showed that SUP and DGC are equally efficient in eliminating spermatozoa containing double-strand DNA damage and sperm with highly damaged DNA, as characterized by the presence of both single-and double-strand DNA breaks.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have investigated the efficacy of these methods with regards to the recovery of reduced percentage of sperm cells with fragmented DNA, however the results are still controversial [30,31,36,37]. While one study showed that the DGC is more effective than SUP in reducing the percentage of sperm with DNA damage [25], another one, in contrast, demonstrated that SUP recovers lower percentage of sperm with fragmented DNA as compared to DGC [37].…”

Section: Discussionmentioning

confidence: 99%

Intracytoplasmic morphologically selected sperm injection outcomes: the role of sperm preparation techniques

Borges

Setti

Vingris

et al. 2013

J Assist Reprod Genet

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Purpose To compare the results of intracytoplasmic morphologically selected sperm injection (IMSI) between cycles in which the swim-up (SUP) or the density gradient centrifugation (DGC) techniques were used for sperm preparation. Methods We evaluated 70 IMSI cycles performed in women with age ≤ 37 years, undergoing IMSI as result of male factor. The couples were divided into two groups: DGC group (n=26) and SUP group (n=44). The groups were compared with regard to IMSI outcomes. Results There were no significant differences between SUP and DGC groups regarding the number of follicles, oocytes, mature oocytes, oocyte yield and mature oocyte rate. Fertilization rate and high-quality embryos rate on day 5 of development were similar between SUP and DGC groups. Implantation, pregnancy and miscarriage rates were not statistically different between SUP and DGC groups (28.8 vs 33.3 %, 46.2 vs 57.1 % and 8.3 vs 4.2 %, respectively). Conclusions Both the SUP and the DGC techniques recover improved sperm fractions and result in similar IMSI outcomes. Further randomized trials analyzing both the quality of sperm through MSOME and the IMSI outcomes are needed to elucidate the role of sperm preparation techniques and morphology on IMSI outcomes.

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“…These data are important because exposing spermatozoa to high levels of ROS may cause DNA fragmentation, which may have adverse consequences if they are used for assisted reproductive techniques (ARTs) (45). This may be true particularly in light of a recent report by Zini and co-workers (46), who found that the improvement in sperm motility after Percoll processing was not associated with a similar improvement in sperm DNA integrity. Those investigators recommended that the current sperm preparation techniques be reevaluated with the goal of minimizing sperm DNA damage.…”

Section: Reactive Oxygen Species and Male Infertilitymentioning

confidence: 99%

Role of reactive oxygen species in the pathophysiology of human reproduction

Agarwal

Saleh

Bedaiwy

2003

Fertility and Sterility

1,236

906

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Objective: To summarize the role of reactive oxygen species (ROS) in the pathophysiology of human reproduction. Design: Review of literature. Setting: Fertility research center and obstetrics and gynecology department in a tertiary care facility. Result(s): ROS plays an essential role in the pathogenesis of many reproductive processes. In male-factor infertility, oxidative stress attacks the fluidity of the sperm plasma membrane and the integrity of DNA in the sperm nucleus. Reactive oxygen species induced DNA damage may accelerate the process of germ cell apoptosis, leading to the decline in sperm counts associated with male infertility. ROS mediated female fertility disorders share many pathogenic similarities with the ones on the male side. These similarities include a potential role in the pathophysiology of endometriosis and unexplained infertility. High follicular fluid ROS levels are associated with negative IVF outcomes, particularly in smokers. Moreover, oxidative stress may be responsible in hydrosalpingeal fluid mediated embryotoxicity as well as poor in vitro embryonic development. Conclusion(s):High levels of ROS are detrimental to the fertility potential both in natural and assisted conception states. (Fertil Steril 2003;79:829 -43.

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Influence of semen processing technique on human sperm DNA integrity

Cited by 179 publications

References 19 publications

Effect of leukocytospermia and processing by discontinuous density gradient on sperm nuclear DNA fragmentation and mitochondrial activity

Effect of leukocytospermia and processing by discontinuous density gradient on sperm nuclear DNA fragmentation and mitochondrial activity

Intracytoplasmic morphologically selected sperm injection outcomes: the role of sperm preparation techniques

Role of reactive oxygen species in the pathophysiology of human reproduction

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