Influence of selenite on selenium uptake, differential antioxidant performance and gene expression of sulfate transporters in wheat genotypes

Inostroza‐Blancheteau, Claudio; Reyes‐Díaz, Marjorie; Alberdi, Miren; Godoy, Karina; Rojas‐Lillo, Yesenia; Cartes, Paula; Mora, Marı́a de la Luz

doi:10.1007/s11104-012-1492-0

Cited by 17 publications

(12 citation statements)

References 61 publications

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“…C, D). From our results and the reported conclusions of Inostroza‐Blancheteau et al (), we infer that NO plays a role in up‐regulating the phosphate and sulfate transporters and thereafter leads to a higher Se concentration of rice seedling roots. Surprisingly, neither NO nor Se IV had any positive effect on the expression of OsNIP2;1 (Fig.…”

Section: Discussionsupporting

confidence: 83%

“…NO, as a ubiquitous signaling molecule, has been extensively studied in plants ( Parani et al, ; Mur et al, ). Although Zhang et al () reported that higher transcription of OsPT2 resulted a significant enhancement of Se concentration of rice seedlings and Inostroza‐Blancheteau et al () found that the gene transcription of these sulfate transporters were enhanced after treatment with SeO 3 2− and then caused Se accumulation in wheat roots, they did not pay any attention to the role of NO. The present study is the first exploration on the role of NO in Se IV uptake and metabolism in plants.…”

Section: Discussionmentioning

confidence: 99%

“…A recent study confirmed that OsPT2, the most abundantly expressed phosphate transporter in roots, was involved in active uptake of SeO 3 2− in rice seedlings ( Zhang et al, ). Interestingly, expression of Sultr4.1 (encoding a sulfate transporter) was up‐regulated and Se was mostly accumulated in wheat roots when SeO 3 2− was applied ( Inostroza‐Blancheteau et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Nitric oxide enhances selenium concentration by promoting selenite uptake by rice roots

Xiao

Gao

et al. 2017

J. Plant Nutr. Soil Sci.

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Nitric oxide (NO) plays a key role in regulating a wide range of physiological and biochemical processes. This study investigated possible effects of NO, which was supplied by sodium nitroprusside (SNP), NO donor, on selenium (Se) concentration, Se species within roots, activities and gene transcription of selenite uptake, and metabolism‐related transporters or enzymes using ICP‐MS, HPLC‐ICP/MS, qRT‐PCR, fluorescence imaging, and biochemical analysis in the roots of rice (Oryza sativa L.) seedling. A suitable SNP concentration (10 µM) increased Se concentration in rice roots. NO enhanced the gene transcription of phosphate transporter OsPT2 and sulfate transporters OsSultr1;2 and OsSultr4;1 that contribute to selenite uptake. Furthermore, NO increased selenocysteine and methyl‐selenocysteine concentrations in roots, stimulated glutathione biosynthesis by up‐regulating the transcript levels of γ‐glutamylcysteine synthetase (γ‐ECS) and glutathione synthetase (GS), and increased cysteine synthase (CS) activity. In conclusion, our results indicate that NO promotes Se concentration of rice seedling roots by enhancing selenite uptake, up‐regulating gene transcription of selenite uptake‐related transporters, and increasing Se metabolism through regulating glutathione biosynthesis.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Nitric oxide enhances selenium concentration by promoting selenite uptake by rice roots

Xiao

Gao

et al. 2017

J. Plant Nutr. Soil Sci.

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“…Therefore, it is questionable if long-term selenium stress and growth impairment in crops is more attributable to photosynthetic damage or rather the increased flux of sugars into respiration and the OPPP to satisfy plants’ ATP and NADPH quota for maintenance costs associated with oxidative stress. Se-tolerance in cultivars of wheat [ 48 ] and ryegrass [ 49 ] are associated with its antioxidant capacity, which is regulated by mitochondrial processes [ 50 ]. In this context it is worth noting that Brassica species are generally considered as Se accumulators that are more Se-tolerant compared to Arabidopsis, wheat, and ryegrass [ 18 , 51 ].…”

Section: Discussionmentioning

confidence: 99%

Selenite activates the alternative oxidase pathway and alters primary metabolism in Brassica napus roots: evidence of a mitochondrial stress response

Dimkovikj

Hoewyk

2014

BMC Plant Biol

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BackgroundHuman requirements for dietary selenium are met mainly by crops. However, excessive uptake of selenium in plants can restrict growth, and its toxicity has been postulated to target roots. Selenite toxicity can be attributed to its assimilation into selenocysteine, which can replace cysteine to yield malformed selenoproteins. Additionally, selenite has pro-oxidant properties. In this study, the effects of selenite on root tissue in Brassica napus (canola) were investigated to better understand its mode of toxicity and the metabolic adjustments needed to mediate a selenite-response.ResultsSelenite induced the rapid formation of mitochondrial superoxide, which led to decreased aconitase activity and involvement of the alternative oxidase pathway. Although selenite altered primary metabolism, as observed by the increased amino acids and decreased TCA cycle metabolites, increased glucose presumably supported higher respiratory rates and ATP levels reported in this study. Additionally, evidence is presented indicating that selenite suppressed the ubiquitin-proteasome pathway, and induced the pentose phosphate pathway needed to maintain antioxidant metabolism. Selenite treatment also elevated glutathione concentration and coincided with increased levels of γ-glutamyl cyclotransferase, which may possibly degrade selenium metabolites conjugated to glutathione.ConclusionCollectively, the data indicate that selenite necessitates the reconfiguration of metabolic pathways to overcome the consequences of mitochondrial oxidative stress in root tissue. Efforts to mitigate the detrimental effects of selenite-induced oxidative stress may ultimately improve selenium tolerance and accumulation in crops.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-014-0259-6) contains supplementary material, which is available to authorized users.

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“…Previous studies suggested that Se in soils can be taken up by the roots through high-affinity sulfate transporters (HASTs), such as AtSULRT1;1 and AtSULRT1;2 transporters [67]. The transcription levels of genes encoding homologs of SULRT1;1 and SULRT1;2 markedly increases in the Se non-accumulator plant species when tissue Se concentration is elevated [68]. However, these genes are constitutively expressed at a relatively high level in the roots of Se hyperaccumulator species [69], accounting for their higher Se capacity.…”

Section: Bf06 Vocs Regulates Plant Uptake Of Se By Upregulating Of Sumentioning

confidence: 99%

Enhanced Iron and Selenium Uptake in Plants by Volatile Emissions of Bacillus amyloliquefaciens (BF06)

et al. 2017

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Volatile organic compounds (VOCs) released by plant growth-promoting rhizobacteria (PGPR) are involved in promoting growth and triggering systemic resistance (ISR) in plants. Importantly, the release of VOCs by some PGPR strains confers improved plant uptake of nutrient elements from the soil. However, the underlying mechanisms of VOCs-regulated nutrient acquisition remain elusive. In this study, VOCs were extracted and identified from Bacillus amyloliquefaciens (strain BF06) using gas chromatography-mass spectrometry (GC-MS). BF06 VOCs exposure significantly promoted the growth and photosynthesis of Arabidopsis plants. To explore how microbial VOCs stimulate growth in plants, gene expression profiles of Arabidopsis seedlings exposed to BF06 VOCs were examined using transcriptomic analyses. In screening differentially expressed genes (DEGs), most upregulated DEGs were found to be related to amino acid transport, iron (Fe) uptake and homeostasis, and sulfate transport. Furthermore, BF06 VOCs significantly enhanced Fe absorption in plants under Fe-limited conditions. However, when nitric oxide (NO) synthesis was inhibited, BF06 VOCs exposure could not substantially augment Fe acquisition in plants under alkaline stress, indicating that VOCs-mediated plant uptake of Fe was required for induction of root NO accumulation. In addition, BF06 VOCs exposure led to a marked increase in some genes encoding for sulfate transporters, and further increased Se accumulation in plants. Intriguingly, BF06 VOCs exposure failed to increase Se uptake in sultr1;2 mutants, which may indicate that high-level transcription of these sulfate transporters induced by BF06 VOCs was essential for enhancing Se absorption by plants. Taken together, our results demonstrated the potential of VOCs released by this strain BF06 to increase Fe and Se uptake in plants.

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Influence of selenite on selenium uptake, differential antioxidant performance and gene expression of sulfate transporters in wheat genotypes

Cited by 17 publications

References 61 publications

Nitric oxide enhances selenium concentration by promoting selenite uptake by rice roots

Nitric oxide enhances selenium concentration by promoting selenite uptake by rice roots

Selenite activates the alternative oxidase pathway and alters primary metabolism in Brassica napus roots: evidence of a mitochondrial stress response

Enhanced Iron and Selenium Uptake in Plants by Volatile Emissions of Bacillus amyloliquefaciens (BF06)

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