Influence of seasonal and ontogenic parameters on the pattern of key terpenoid indole alkaloids biosynthesized in the leaves of Catharanthus roseus

Mall, Maneesha; Verma, Rajesh; Gupta, Madan M.; Shasany, Ajit Kumar; Khanuja, S. P. S.; Shukla, Ashutosh K.

doi:10.1016/j.sajb.2019.01.032

Cited by 19 publications

(11 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Catharanthus roseus is rich in alkaloids, of which vindoline and catharanthine are the key components in the synthesis of anticancer substances vinblastine and vincristine, and their high yields from the plant facilitate economical bisindole production semi-synthetically [ 30 ]. As an elicitor, chitooligosaccharides have a low molecular weight and can be recognized as stress signals by plant cells easily [ 31 ].…”

Section: Resultsmentioning

confidence: 99%

Enhancement of Vindoline and Catharanthine Accumulation, Antioxidant Enzymes Activities, and Gene Expression Levels in Catharanthus roseus Leaves by Chitooligosaccharides Elicitation

Tang

Liu

et al. 2022

Marine Drugs

View full text Add to dashboard Cite

Catharanthus roseus (L.) G. Don is a plant belonging to the genus Catharanthus of the Apocynaceae family. It contains more than one hundred alkaloids, of which some exhibit significant pharmacological activities. Chitooligosaccharides are the only basic aminooligosaccharides with positively charged cations in nature, which can regulate plant growth and antioxidant properties. In this study, the leaves of Catharanthus roseus were sprayed with chitooligosaccharides of different molecular weights (1 kDa, 2 kDa, 3 kDa) and different concentrations (0.01 μg/mL, 0.1 μg/mL, 1 μg/mL and 10 μg/mL). The fresh weights of its root, stem and leaf were all improved after chitooligosaccharides treatments. More importantly, the chitooligosaccharides elicitor strongly stimulated the accumulation of vindoline and catharanthine in the leaves, especially with the treatment of 0.1 μg/mL 3 kDa chitooligosaccharides, the contents of them were increased by 60.68% and 141.54%, respectively. Furthermore, as the defensive responses, antioxidant enzymes activities (catalase, glutathione reductase, ascorbate peroxidase, peroxidase and superoxide dismutase) were enhanced under chitooligosaccharides treatments. To further elucidate the underlying mechanism, qRT-PCR was used to investigate the genes expression levels of secologanin synthase (SLS), strictosidine synthase (STR), strictosidine glucosidase (SGD), tabersonine 16-hydroxylase (T16H), desacetoxyvindoline-4-hydroxylase (D4H), deacetylvindoline-4-O-acetyltransferase (DAT), peroxidase 1 (PRX1) and octadecanoid-responsive Catharanthus AP2-domain protein 3 (ORCA3). All the genes were significantly up-regulated after chitooligosaccharides treatments, and the transcription abundance of ORCA3, SLS, STR, DAT and PRX1 reached a maximal level with 0.1 μg/mL 3 kDa chitooligosaccharides treatment. All these results suggest that spraying Catharanthus roseus leaves with chitooligosaccharides, especially 0.1 μg/mL of 3 kDa chitooligosaccharides, may effectively improve the pharmaceutical value of Catharanthus roseus.

show abstract

Section: Resultsmentioning

confidence: 99%

Enhancement of Vindoline and Catharanthine Accumulation, Antioxidant Enzymes Activities, and Gene Expression Levels in Catharanthus roseus Leaves by Chitooligosaccharides Elicitation

Tang

Liu

et al. 2022

Marine Drugs

View full text Add to dashboard Cite

show abstract

“…Findings of increased production and accumulation of alkaloids during drought have been shown for plants from different families, for example, in Catharantus roseus [35][36][37][38] Papaver somniferum [16], Lycoris aurea [39], Datura stramonium [40], and Pinellia ternata [41]. Although the authors do not directly report the reason for these increases, some point out that alkaloids could be part of the protection strategies for plants under water stress.…”

Section: Discussionmentioning

confidence: 99%

Liriodenine and Its Probable Role as an Osmolyte during Water Stress in Annona lutescens (Annonaceae)

Cisneros-Andrés,

Cruz-Ortega,

Castro-Moreno

et al. 2024

IJPB

View full text Add to dashboard Cite

In tropical deciduous forests (TDFs), plants have developed various strategies to tolerate desiccation during the dry season. One strategy is osmotic adjustment, which includes the accumulation of secondary metabolites. Annona lutescens, a species that inhabits TDFs, increases and accumulates liriodenine alkaloid in its roots during the dry season. In this study, we evaluate the possible role of this molecule as an osmolyte and in pH homeostasis. We performed growth analyses and determined liriodenine concentrations during water stress in Annona lutescens seedlings grown under controlled temperature, water, and light conditions. We also calculated their osmotic adjustment based on pressure–volume curves and performed solubility tests along a pH gradient. Osmotic adjustment was compared between control plants (irrigated) and plants subjected to 15, 25, and 35 days of water stress. Osmotic adjustment was dramatically higher in plants subjected to 35 days of water stress compared to the control. The solubility of liriodenine was 54% at pH 4.5, and when liriodenine was in contact with malic acid solutions, the pH increased slightly. The highest concentration of liriodenine was in the roots, with a significant increase from 540.855 μg g−1 after 15 days of water stress to 1239.897 μg g−1 after 35 days. Our results suggest that liriodenine plays an important role in the response to water stress as an osmolyte and in pH homeostasis.

show abstract

“…This attracted the attention of another family member, CrTIL. This was relevant since temperature-responsiveness or seasonal patterning of vindoline biosynthesis/accumulation in C. roseus is known (Mall et al, 2019), which makes it pertinent to characterize genes encoding temperature-dependant proteins. Such proteins may be directly/ indirectly involved in the accumulation/retention of specific TIAs, such as vindoline.…”

Section: Responses To Abiotic Factorsmentioning

confidence: 99%

Temperature‐induced lipocalin‐mediated membrane integrity: Possible implications for vindoline accumulation in Catharanthus roseus leaves

Mall,

Shanker,

Nagegowda

et al. 2023

Physiologia Plantarum

Self Cite

View full text Add to dashboard Cite

Plant lipocalins perform diverse functions. Recently, allene oxide cyclase, a lipocalin family member, has been shown to co‐express with vindoline pathway genes in Catharanthus roseus under various biotic/abiotic stresses. This brought focus to another family member, a temperature‐induced lipocalin (CrTIL), which was selected for full‐length cloning, tissue‐specific expression profiling, in silico characterization, and upstream genomic region analysis for cis‐regulatory elements. Stress‐mediated variations in CrTIL expression were reflected as disturbances in cell membrane integrity, assayed through measurement of electrolyte leakage and lipid peroxidation product, MDA, which implicated the role of CrTIL in maintaining cell membrane integrity. For ascertaining the function of CrTIL in maintaining membrane stability and elucidating the relationship between CrTIL expression and vindoline content, if any, a direct approach was adopted, whereby CrTIL was transiently silenced and overexpressed in C. roseus. CrTIL silencing and overexpression confirmed its role in the maintenance of membrane integrity and indicated an inverse relationship of its expression with vindoline content. GFP fusion‐based subcellular localization indicated membrane localization of CrTIL, which was in agreement with its role in maintaining membrane integrity. Altogether, the role of CrTIL in maintaining membrane structure has possible implications for the intracellular sequestration, storage, and viability of vindoline.

show abstract

Influence of seasonal and ontogenic parameters on the pattern of key terpenoid indole alkaloids biosynthesized in the leaves of Catharanthus roseus

Cited by 19 publications

References 26 publications

Enhancement of Vindoline and Catharanthine Accumulation, Antioxidant Enzymes Activities, and Gene Expression Levels in Catharanthus roseus Leaves by Chitooligosaccharides Elicitation

Enhancement of Vindoline and Catharanthine Accumulation, Antioxidant Enzymes Activities, and Gene Expression Levels in Catharanthus roseus Leaves by Chitooligosaccharides Elicitation

Liriodenine and Its Probable Role as an Osmolyte during Water Stress in Annona lutescens (Annonaceae)

Temperature‐induced lipocalin‐mediated membrane integrity: Possible implications for vindoline accumulation in Catharanthus roseus leaves

Contact Info

Product

Resources

About