Influence of sampling and storage conditions on plasma renin activity and plasma renin concentration

Lőcsei, Zoltán; Rácz, Kàroly; Patócs, Attila; Kovaćs, Gábor L.; Toldy, Erzsébet

doi:10.1016/j.cca.2009.01.013

Cited by 23 publications

(27 citation statements)

References 19 publications

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“…12 Another study of 12 participants has suggested a decline of over 10% in EDTA plasma at 2 hours compared with immediately processed samples but did not assess stability in serum. 13 Our finding of different renin concentrations in serum gel and EDTA plasma is in contrast to previous research showing similar concentrations of directly measured renin when measured in serum and plasma (n=15). 12 Dilution due to EDTA additive is reported as <1% using this collection tube (Sarstedt, UK).…”

Section: Discussioncontrasting

confidence: 99%

Current laboratory requirements for adrenocorticotropic hormone and renin/aldosterone sample handling are unnecessarily restrictive

et al. 2017

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Samples for adrenocorticotropic hormone (ACTH) and aldosterone/renin analysis usually require rapid transport to the receiving laboratory for immediate separation and freezing. In practice, this means assessment is limited to hospital settings and many samples are rejected. We examined whether these requirements are necessary by assessing the stability of ACTH, aldosterone and renin over 48 hours in whole blood collected in serum gel and EDTA plasma from 31 participants. Our results show that ACTH collected into EDTA plasma is stable at room temperature for at least 6 hours, mean change at 6 hours -2.6% (95% CI -9.7 to 4.5). Both aldosterone and renin were stable collected on serum gel at room temperature for at least 6 hours: mean change aldosterone +0.2% (95% CI -3.6 to 4.0), renin -1.9% (95% CI -7.0 to3.2). Therefore, by using appropriate preservatives, ACTH and aldosterone/renin can be measured on samples collected at room temperature and processed within 6 hours. This would facilitate outpatient and emergency room assessment of these analytes.

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Section: Discussioncontrasting

confidence: 99%

Current laboratory requirements for adrenocorticotropic hormone and renin/aldosterone sample handling are unnecessarily restrictive

et al. 2017

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“…8 From our study it appears that when processing blood samples, cool or room temperature during the initial 20-30 minutes has no significant effect on the results of direct renin, and to a lesser extent, on PRA. Therefore, a refrigerated centrifuge is not necessary at the site of blood drawing, if the blood samples can be further processed either in ambient or cool conditions within 20-30 minutes in the local laboratory.…”

Section: Discussionmentioning

confidence: 56%

“…Therefore, a refrigerated centrifuge is not necessary at the site of blood drawing, if the blood samples can be further processed either in ambient or cool conditions within 20-30 minutes in the local laboratory. Otherwise, according to suggestions by Locsei et al, they could be placed at 0-5°C and transported to a laboratory in less than two hours 8 or possibly quickly centrifuged and transported frozen on dry ice.…”

Section: Discussionmentioning

confidence: 99%

The effect of blood collection procedure on plasma renin activity (PRA) and concentrations of direct renin (DRC) and aldosterone

Glinicki

Jeske

Gietka-Czernel

et al. 2013

J Renin Angiotensin Aldosterone Syst

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Introduction: Performing measurements of plasma renin activity (PRA) or direct renin concentration (DRC) and aldosterone concentration, we should be well informed about requirements concerning blood sample processing. Material and methods: Forty-seven patients had blood collected in the supine and upright positions. Blood was withdrawn into two EDTA 2 K tubes and one with clot activator. One EDTA 2 K tube was cooled at +4°C and centrifuged at +4°C whereas the other was prepared at room temperature. PRA and DRC were measured by radioimmunoassay (RIA) and radioimmunometry (IRMA), respectively, in both cooled and not cooled plasma samples, and aldosterone was measured by RIA in not cooled plasma and in serum. Results: In all the groups, with low, medium, and high values of PRA and direct renin, the temperature of sample processing within 30 minutes had no marked influence on the final result (correlation coefficient for renin was 0.9994, and for PRA, 0.8297). The measured concentrations of aldosterone also showed high correlation (r = 0.9790) but were markedly higher in plasma. Conclusion:The measurements of DRC, and to a lesser extent PRA, were similar regardless of temperature condition during the 20-30 minutes necessary for blood sample processing. Aldosterone concentrations in plasma vs serum samples appeared to be markedly higher.

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“…The reported range for low to high angiotensin I production in plasma is 0.2 ng/mL/h to 13.5 ng/mL/h, respectively, with an average of 1.6 ng/mL/h [14]. A 1-h angiotensin I generation period would result in a final plasma concentration of ϳ150 to 10,400 attomol/ L. Even with our current version of the assay, which measures the amount of angiotensin in one-fifth of the original 20 L of plasma in each well on the MALDI plate, the sensitivity of the iMALDI assay should be sufficient to detect lower levels of angiotensin I, especially if detergent-free beads were used.…”

Section: Comparison Of Imaldi and Ria For Determination Of Pramentioning

confidence: 99%

Towards the development of an immuno MALDI (iMALDI) mass spectrometry assay for the diagnosis of hypertension

Reid

Holmes

Mason

et al. 2010

J. Am. Soc. Mass Spectrom.

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The renin-angiotensin-aldosterone system (RAAS) plays an essential role in the regulation of plasma volume and arterial blood pressure. One of the most common diseases of the RAAS is the autonomous production of aldosterone by the adrenal glands, caused by either bilateral adrenal hyperplasia or an aldosterone-producing adenoma. This condition, known as primary aldosteronism, is a treatable and often curable form of hypertension. The measurement of plasma renin activity (PRA), as determined by radioimmunoassay for angiotensin I is essential to the diagnosis of primary aldosteronism. However, accurate determination of PRA is often hampered by low plasma concentrations of angiotensin I. Here, we report the use of immuno-MALDI (iMALDI) as a highly sensitive and specific method for the absolute quantitation of angiotensin I in plasma. iMALDI permits concentration determination by affinity-capture of angiotensin I and a stable-isotopically labeled standard (SIS) peptide on immobilized antipeptide antibodies. The affinity beads are placed on the MALDI target, permitting automated analysis of large numbers of patient samples. Pretreatment of the plasma is not required, and this method is suitable for the accurate determination of angiotensin I in whole plasma. The calibration curve generated using this method was linear over a 50-fold concentration range in plasma, with a correlation coefficient of 0.984. MS/MS sequence confirmation provides absolute specificity. The iMALDI angiotensin I assay, therefore, has the potential to be developed into a method for determining PRA that has advantages in time, in specificity, and in safety. (J Am Soc Mass Spectrom 2010, 21, 1680 -1686) © 2010 American Society for Mass Spectrometry H ypertension is a serious and often symptomless condition that currently affects ϳ1 billion people [1]. Because hypertension increases the chance of heart attack, heart failure, stroke, and kidney disease, it is imperative that treatable and/or curable forms of hypertension be identified early and appropriately managed. The renin-angiotensin-aldosterone system (RAAS) plays an important role in the regulation of aldosterone, and consequently, maintenance of arterial pressure and development of certain hypertensive diseases. This system is regulated by the proteolytic enzyme renin, which is released into the plasma from the juxtaglomerular cells of the afferent arteriole of the renal glomerulus in response to low arterial pressure, direct adrenergic stimulation or reductions in Na ϩ or Cl -concentrations in renal tubular fluid [2]. Renin cleaves the decapeptide angiotensin I from angiotensinogen, an ␣-2 globular protein, which is then converted into an octapeptide, angiotensin II, principally but not exclusively by the action of angiotensin converting enzyme (ACE). While angiotensin I has no physiologic effects, angiotensin II is a powerful vasoconstrictor and regulator of sodium reabsorption and water retention, thereby increasing blood pressure. Angiotensin II also stimulates the release of aldosteron...

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Influence of sampling and storage conditions on plasma renin activity and plasma renin concentration

Cited by 23 publications

References 19 publications

Current laboratory requirements for adrenocorticotropic hormone and renin/aldosterone sample handling are unnecessarily restrictive

Current laboratory requirements for adrenocorticotropic hormone and renin/aldosterone sample handling are unnecessarily restrictive

The effect of blood collection procedure on plasma renin activity (PRA) and concentrations of direct renin (DRC) and aldosterone

Towards the development of an immuno MALDI (iMALDI) mass spectrometry assay for the diagnosis of hypertension

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