Influence of Salmonella enterica Serovar Enteritidis Infection on the Development of the Cecum Microbiota in Newly Hatched Chicks

Juřicová, Helena; Vídeňská, Petra; Lukač, Maja; Faldynová, Marcela; Babák, Vladimír; Havlíčková, Hana; Šišák, František; Rychlı́k, Ivan

doi:10.1128/aem.02628-12

Cited by 63 publications

(57 citation statements)

References 14 publications

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“…These results indicate that S. typhimurium infection influenced the composition of the cecal microbiota in chickens. The changes in the composition of gut microbiota were also observed in animals infected with non-typhoid serovars of Salmonella (Barman et al, 2008;Juricova et al, 2013;Videnska et al, 2013), which were associated with inflammation (Stecher et al, 2007;Barman et al, 2008). Thus, these changes in the microbiotal composition induced by S. typhimurium infection, as observed in our study, may contribute to the processes of mucosal injury (Tamboli et al, 2004;Sokol et al, 2006), and support the observed results of a reduction in the villus-height/crypt-depth ratio of the ileum and an elevated apoptotic index of ileal epithelial cells in challenged broilers.…”

Section: Discussionmentioning

confidence: 81%

“…At the initiation of intestinal infection of broiler chickens, S. typhimurium must outcompete the resident microbiota of the distal ileum and cecum and penetrate the mucosal epithelium (Berndt et al, 2007). After overcoming colonization resistance, S. typhimurium disrupts the intestinal ecosystem in mammals and chickens, leading to infection of the gastrointestinal tract (Stecher et al, 2007;Barman et al, 2008;Juricova et al, 2013;Videnska et al, 2013). Furthermore, Salmonella infections can cause infected epithelial cell shedding from the villus surface, resulting in villus blunting and loss of absorptive surfaces (Wallis and Galyov, 2000).…”

Section: Introductionmentioning

confidence: 99%

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Effect of zinc on growth performance, gut morphometry, and cecal microbial community in broilers challenged with Salmonella enterica serovar typhimurium

et al. 2014

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To evaluate the effects of supplemental zinc on growth performance, gut morphometry, and the cecal microbial community in broilers challenged with Salmonella typhimurium, 180, 1-day-old male Cobb 500 broiler chicks were randomly assigned to 3 treatments with ten replicates for a 42 day experiment. The 3 treatments were: unchallenged, S. typhimurium-challenged, and S. typhimurium-challenged with 120 mg/kg of zinc supplementation in the diet. Salmonella infection caused a reduction in body-weight gain and feed intake, disrupted the intestinal structure by decreasing the villus-height/crypt-depth ratio of the ileum and increasing the apoptotic index of ileal epithelial cells. Moreover, the cecal microbial community was altered by Salmonella infection, as demonstrated by a reduced number of Lactobacillus and total bacteria. Dietary zinc supplementation improved growth performance by increasing the body-weight gain and feed intake in the challenged broilers. In addition, zinc repaired intestinal injury by reducing the apoptotic index of ileal epithelial cells, enhancing villus height and the villus-height/crypt-depth ratio of the ileum, and the proliferation index of ileal epithelial cells. Finally, zinc regulated the cecal microbial community by increasing the number of total bacteria and beneficial Lactobacillus bacteria, and reducing the number of Salmonella. The results indicated that dietary zinc supplementation improved growth performance, intestinal morphology, and intestinal microbiota in S. typhimurium-challenged broilers.

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Section: Discussionmentioning

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Effect of zinc on growth performance, gut morphometry, and cecal microbial community in broilers challenged with Salmonella enterica serovar typhimurium

et al. 2014

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“…After PCR, the Ct values of the genes of interest were subtracted from an average Ct value of amplifications performed with universal primer pairs for the domain Bacteria (ΔCt). The relative amount of each taxon in the total bacterial population was finally calculated as 2 -ΔCt [15,16]. …”

Section: Methodsmentioning

confidence: 99%

Chicken faecal microbiota and disturbances induced by single or repeated therapy with tetracycline and streptomycin

et al. 2013

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BackgroundIn this study, we characterised the microbiota present in the faeces of 15- and 46-week-old egg laying hens before and after tetracycline or streptomycin therapy. In the first experiment, the layers were subjected to 7 days of therapy. In the second experiment, the hens were subjected to two days of therapy, which was repeated for an additional two days after 12 days of antibiotic withdrawal. This enabled us to characterise dynamics of the changes after antibiotic administration and withdrawal, and to identify genera repeatedly resistant to tetracycline and streptomycin.ResultsReal-time PCRs specific for Enterobacteriales, Lactobacillales, Clostridiales and Bifidobacteriales showed that changes in the microbiota in response to antibiotic therapy and antibiotic withdrawal were quite rapid and could be observed within 24 hours after the change in therapy status. Pyrosequencing of PCR amplified V3/V4 variable regions of 16S rRNA genes showed that representatives of the orders Clostridiales, Lactobacillales, Bacteroidales, Bifidobacteriales, Enterobacteriales, Erysipelotrichales, Coriobacteriales, Desulfovibrionales, Burkholderiales, Campylobacterales and Actinomycetales were detected in the faeces of hens prior to the antibiotic therapy. Tetracycline and streptomycin therapies decreased the prevalence of Bifidobacteriales, Bacteroidales, Clostridiales, Desulfovibrionales, Burkholderiales and Campylobacterales in faecal samples in both experiments. On the other hand, Enterobacteriales and Lactobacillales always increased in prevalence in response to both therapies. Within the latter two orders, Escherichia and Enterococcus were the genera prevalence of which increased after all the antibiotic treatments.ConclusionsThe changes in microbiota composition induced by the antibiotic therapy were rapid and quite dramatic and only representatives of the genera Enterococcus and Escherichia increased in response to the therapy with both antibiotics in both experiments.

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“…We were therefore interested whether the inflammation induced by Salmonella infection will or will not affect the development and composition of gut microbiota in chickens. Our previous results using T-RFLP and quantitative real time PCR indicated that the changes in gut microbiota in chickens are not as dramatic as one would expect from data in other models of host - pathogen interactions [12-14,16]. In this study we have therefore used pyrosequencing of V3 and V4 variable regions of 16S rRNA genes to characterize the consequences of S .…”

Section: Introductionmentioning

confidence: 96%

Influence of Salmonella enterica serovar Enteritidis infection on the composition of chicken cecal microbiota

et al. 2013

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BackgroundInfection of newly hatched chicks with Salmonella enterica serovar Enteritidis (S. Enteritidis) results in an inflammatory response in the intestinal tract which may influence the composition of gut microbiota. In this study we were therefore interested whether S. Enteritidis induced inflammation results in changes in the cecal microbiota. To reach this aim, we compared the cecal microbiota of non-infected chickens and those infected by S. Enteritidis by pyrosequencing the V3/V4 variable regions of genes coding for 16S rRNA.ResultsCecal microbiota of chickens up to 19 days of life was dominated by representatives of Enterobacteriaceae, Lachnospiraceae and Ruminococcaceae, followed by Lactobacillaceae. The presence of Lachnospiraceae did not change after S. Enteritidis infection. Enterobacteriaceae increased and Ruminococcaceae decreased after S. Enteritidis infection in two independent experiments although these results were not significant. A significant increase in both experiments was observed only for the representatives of Lactobacillaceae which may correlate with their microaerophilic growth characteristic compared to the obligate anaerobes from the families Lachnospiraceae and Ruminococcaceae.ConclusionsWe conclude that S. Enteritidis infection influences the composition of the cecal microbiota in chickens but these changes are minor in nature and should be understood more as an indirect consequence of infection and inflammation rather than a positively selected evolutionary trait.

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Influence of Salmonella enterica Serovar Enteritidis Infection on the Development of the Cecum Microbiota in Newly Hatched Chicks

Cited by 63 publications

References 14 publications

Effect of zinc on growth performance, gut morphometry, and cecal microbial community in broilers challenged with Salmonella enterica serovar typhimurium

Effect of zinc on growth performance, gut morphometry, and cecal microbial community in broilers challenged with Salmonella enterica serovar typhimurium

Chicken faecal microbiota and disturbances induced by single or repeated therapy with tetracycline and streptomycin

Influence of Salmonella enterica serovar Enteritidis infection on the composition of chicken cecal microbiota

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