Influence of salinity on spermatogenesis in adult Nile tilapia (Oreochromis niloticus) testis

Vieira, Augusto Bicalho Cruz; Weber, André Alberto; Ribeiro, Yves Moreira; Luz, Ronald Kennedy; Bazzoli, Nilo; Rizzo, Elizete

doi:10.1016/j.theriogenology.2019.03.013

Cited by 19 publications

(12 citation statements)

References 55 publications

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“…Moreover, a global significant increase in overall histone PTM abundance across the entire chromatin does not exclude the possibility of a significant reduction at specific genomic loci. Nonetheless, the decreased global relative abundance of H3K14ac observed in the testes of Mozambique tilapia is consistent with a recent study conducted on Nile tilapia ( Oreochromis niloticus ) [ 38 ]. When Nile tilapia were exposed to salinities near their upper tolerance limit, they experienced only subtle changes in spermatogenesis, but exhibited protein-level changes in the heat shock protein 70 (HSP70) and proliferating cell nuclear antigen (PCNA) in their testes [ 38 ].…”

Section: Discussionsupporting

confidence: 91%

“…Nonetheless, the decreased global relative abundance of H3K14ac observed in the testes of Mozambique tilapia is consistent with a recent study conducted on Nile tilapia ( Oreochromis niloticus ) [ 38 ]. When Nile tilapia were exposed to salinities near their upper tolerance limit, they experienced only subtle changes in spermatogenesis, but exhibited protein-level changes in the heat shock protein 70 (HSP70) and proliferating cell nuclear antigen (PCNA) in their testes [ 38 ]. As salinity increased, the abundance of HSP70 decreased, and the abundance of PCNA increased [ 38 ].…”

Section: Discussionsupporting

confidence: 91%

“…When Nile tilapia were exposed to salinities near their upper tolerance limit, they experienced only subtle changes in spermatogenesis, but exhibited protein-level changes in the heat shock protein 70 (HSP70) and proliferating cell nuclear antigen (PCNA) in their testes [ 38 ]. As salinity increased, the abundance of HSP70 decreased, and the abundance of PCNA increased [ 38 ]. The abundance of HSP70 has previously been found to exhibit a positive correlation with H3K14ac [ 39 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

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Salinity-responsive histone PTMs identified in the gills and gonads of Mozambique tilapia (Oreochromis mossambicus)

Mojica,

Fu,

Kültz

2024

BMC Genomics

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Background Histone post-translational modifications (PTMs) are epigenetic marks that can be induced by environmental stress and elicit heritable patterns of gene expression. To investigate this process in an ecological context, we characterized the influence of salinity stress on histone PTMs within the gills, kidney, and testes of Mozambique tilapia (Oreochromis mossambicus). A total of 221 histone PTMs were quantified in each tissue sample and compared between freshwater-adapted fish exposed to salinity treatments that varied in intensity and duration. Results Four salinity-responsive histone PTMs were identified in this study. When freshwater-adapted fish were exposed to seawater for two hours, the relative abundance of H1K16ub significantly increased in the gills. Long-term salinity stress elicited changes in both the gills and testes. When freshwater-adapted fish were exposed to a pulse of severe salinity stress, where salinity gradually increased from freshwater to a maximum of 82.5 g/kg, the relative abundance of H1S1ac significantly decreased in the gills. Under the same conditions, the relative abundance of both H3K14ac and H3K18ub decreased significantly in the testes of Mozambique tilapia. Conclusions This study demonstrates that salinity stress can alter histone PTMs in the gills and gonads of Mozambique tilapia, which, respectively, signify a potential for histone PTMs to be involved in salinity acclimation and adaptation in euryhaline fishes. These results thereby add to a growing body of evidence that epigenetic mechanisms may be involved in such processes.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Salinity-responsive histone PTMs identified in the gills and gonads of Mozambique tilapia (Oreochromis mossambicus)

Mojica,

Fu,

Kültz

2024

BMC Genomics

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“…Changes in water salinity modify the homeostasis of the organisms and biological processes and may even lead to death ( Rahmah et al, 2020 ). Exposure of different tilapia species to increasing salinity is known to affect growth performance ( Ninh et al, 2014 ; Gan et al, 2016 ), digestive capacity ( Tran-Ngoc et al, 2017 ), blood parameters ( Verdegem et al, 1997 ), reproductive capacity ( Cruz Vieira et al, 2019 ), histopathology and behavior ( Hassan et al, 2013 ), antioxidant status ( Gan et al, 2016 ), and metabolic rate ( Iwama et al, 1997 ).…”

Section: Introductionmentioning

confidence: 99%

Exposure to salinity induces oxidative damage and changes in the expression of genes related to appetite regulation in Nile tilapia (Oreochromis niloticus)

et al. 2022

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Variations in water salinity and other extrinsic factors have been shown to induce changes in feeding rhythms and growth in fish. However, it is unknown whether appetite-related hormones mediate these changes in Nile tilapia (Oreochromis niloticus), an important species for aquaculture in several countries. This study aimed to evaluate the expression of genes responsible for appetite regulation and genes related to metabolic and physiological changes in tilapia exposed to different salinities. Moreover, the study proposed to sequence and to characterize the cart, cck, and pyy genes, and to quantify their expression in the brain and intestine of the fish by quantitative polymerase chain reaction (qPCR). The animals were exposed to three salinities: 0, 6, and 12 parts per thousand (ppt) of salt for 21 days. Furthermore, lipid peroxidation, reactive oxygen species, DNA damage, and membrane fluidity in blood cells were quantified by flow cytometry. The results indicated an increased expression of cart, pyy, and cck and a decreased expression of npy in the brain, and the same with cck and npy in the intestine of fish treated with 12 ppt. This modulation and other adaptive responses may have contributed to the decrease in weight gain, specific growth rate, and final weight. In addition, we showed oxidative damage in blood cells resulting from increasing salinity. These results provide essential data on O. niloticus when exposed to high salinities that have never been described before and generate knowledge necessary for developing biotechnologies that may help improve the production of economically important farmed fish.

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“…Recent research has suggested that cortisol, mostly associated with stress response via the HPI axis, may act as a key factor linking environmental stimuli to physiological responses during sexual development and reproduction through its cross-talk with the HPG axis, initiating a shift in steroidogenesis from estrogens to androgens [1,3,27]. Therefore, changes in salinity could have major effects on teleost sexual development, such as spermatogenesis and testicular homeostasis, as well as the proliferation and apoptosis of gonadal cells [2,28]. Chinese sea bass (Lateolabrax maculatus) is one of the most popular aquaculture fish species in China due to its high nutritive value and pleasant taste.…”

Section: Introductionmentioning

confidence: 99%

Steroidogenic Effects of Salinity Change on the Hypothalamus–Pituitary–Gonad (HPG) Axis of Male Chinese Sea Bass (Lateolabrax maculatus)

Fang

Wang

et al. 2022

IJMS

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As lower vertebrates, teleost species could be affected by dynamic aquatic environments and may respond to environmental changes through the hypothalamus–pituitary–gonad (HPG) axis to ensure their normal growth and sexual development. Chinese sea bass (Lateolabrax maculatus), euryhaline marine teleosts, have an extraordinary ability to deal with a wide range of salinity changes, whereas the salinity decrease during their sex-maturation season may interfere with the HPG axis and affect their steroid hormone metabolism, resulting in abnormal reproductive functioning. To this end, in this study, 40 HPG axis genes in the L. maculatus genome were systematically characterized and their copy numbers, phylogenies, gene structures, and expression patterns were investigated, revealing the conservation of the HPG axis among teleost lineages. In addition, freshwater acclimation was carried out with maturing male L. maculatus, and their serum cortisol and 11-ketotestosterone (11-KT) levels were both increased significantly after the salinity change, while their testes were found to be partially degraded. After salinity reduction, the expression of genes involved in cortisol and 11-KT synthesis (cyp17a, hsd3b1, cyp21a, cyp11c, hsd11b2, and hsd17b3) showed generally upregulated expression in the head kidneys and testes, respectively. Moreover, cyp11c and hsd11b2 were involved in the synthesis and metabolism of both cortisol and 11-KT, and after salinity change their putative interaction may contribute to steroid hormone homeostasis. Our results proved the effects of salinity change on the HPG axis and steroidogenic pathway in L. maculatus and revealed the gene interactions involved in the regulation of steroid hormone levels. The coordinated interaction of steroidogenic genes provides comprehensive insights into steroidogenic pathway regulation, as well as sexual development, in teleost species.

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Influence of salinity on spermatogenesis in adult Nile tilapia (Oreochromis niloticus) testis

Cited by 19 publications

References 55 publications

Salinity-responsive histone PTMs identified in the gills and gonads of Mozambique tilapia (Oreochromis mossambicus)

Salinity-responsive histone PTMs identified in the gills and gonads of Mozambique tilapia (Oreochromis mossambicus)

Exposure to salinity induces oxidative damage and changes in the expression of genes related to appetite regulation in Nile tilapia (Oreochromis niloticus)

Steroidogenic Effects of Salinity Change on the Hypothalamus–Pituitary–Gonad (HPG) Axis of Male Chinese Sea Bass (Lateolabrax maculatus)

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