Influence of repeated short-term nitrogen limitations on leaf phenolics metabolism in tomato

Larbat, Romain; Olsen, Kristine M.; Slimestad, Rune; Løvdal, Trond; Bénard, Camille; Verheul, Michel; Bourgaud, Frédéric; Robin, Christophe; Lillo, Cathrine

doi:10.1016/j.phytochem.2012.02.004

Cited by 66 publications

(55 citation statements)

References 54 publications

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“…Indeed, we assumed that the N-limited tomato plants produced more insect-defensive soluble phenolics (C-based defensive compounds) i.e. chlorogenic acid, rutin, kaempferol-rutinoside since their concentration is positively correlated with C/N ratio in tomato leaves718192037. Moreover, the concentration of constitutive tomatine, a key N-based glycoalkaloid defensive compound in tomato, is also positively correlated with leaf C/N ratio20.…”

Section: Discussionmentioning

confidence: 99%

“…The explanation could be that (1) insects need to transform the inorganic nitrogen forms present in plant tissue and/or utilize directly plant-derived amino acids to synthesize structural proteins and enzymes5; (2) nitrogen deficient plants have a higher accumulation of plant allelochemicals which can be toxic to herbivorous insects. For instance, tomato plants subjected to nitrogen limitation accumulate more phenolic compounds in their organs17181920. Although many studies reported the existence of positive correlations between insect performance and host plant nitrogen content as mentioned above, several studies however have documented negative effects due to high nitrogen content in leaves and undermined the general application of the “Nitrogen limitation hypothesis”152122.…”

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confidence: 99%

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Nitrogen and water availability to tomato plants triggers bottom-up effects on the leafminer Tuta absoluta

Han

Lavoir

Bot

et al. 2014

Sci Rep

104

106

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This study examined the effects of various levels of nitrogen inputs (optimal, insufficient and excessive) and water inputs (optimal, low drought and high drought) to tomato plants (Solanum lycopersicum) on survival and development of an invasive tomato leafminer, Tuta absoluta (Meytick) (Lepidoptera: Gelechiidae). Plant growth i.e. plant height and the number of nodes declined under insufficient or excessive nitrogen treatment. Compared to optimal N, insufficient N treatment decreased leaf N content and increased the carbon/nitrogen ratio (C/N) whereas an excess of N had no effect on both leaf N content and leaf C/N ratio. Sub-optimal nitrogen supplies, water treatments and their interactions, significantly reduced the leafminer survival rate and slowed down its development. Together with the findings from three recent companion studies, we assumed that a combination of changes in nutritional value and chemical defense could explain these observed effects. Furthermore, our findings supported both the “Plant vigor hypothesis” and the “Nitrogen limitation hypothesis”.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Nitrogen and water availability to tomato plants triggers bottom-up effects on the leafminer Tuta absoluta

Han

Lavoir

Bot

et al. 2014

Sci Rep

104

106

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“…In recent years, many studies have reported the molecular regulation of anthocyanin biosynthesis in response to different grown environments. Larbat reported that the structural genes of phenylalanine ammonialyase (PAL), chalcone synthase (CHS), and flavanone 3-hydroxylase (F3H) significantly increased in answer to nitrogen (N) depletion [11]. Many studies found that the expression of the UDP-glycose flavonoid glycosyl transferase (UFGT) gene is vital to anthocyanin accumulation in many plants, including strawberry, lychee, and grape [12][13].…”

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confidence: 99%

How Exogenous Selenium Affects Anthocyanin Accumulation and Biosynthesis-Related Gene Expression in Purple Lettuce

Liu¹,

Li²,

Wang³

et al. 2017

Pol. J. Environ. Stud.

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“…When the chlorophylls are degraded, the cells become susceptible to oxidative damage by highlight and other photosynthetic pigments are exposed or the novo synthetized to dissipate excess energy, as anthocyanins (Carpenter et al 2014;Kovinich et al 2014). A decrease in proteins, chlorophylls, appearance of chlorosis and anthocyanin production has been also observed in Arabidopsis thaliana and Solanum lycopersicum under N stress (Larbat et al 2012;De Gernier et al 2016).…”

Section: Discussionmentioning

confidence: 96%

MORPHOGENESIS AND SECONDARY METABOLITES PRODUCTION IN THE MEDICINAL PLANT Castilleja tenuiflora Benth. UNDER NITROGEN DEFICIENCY AND STARVATION STRESS IN A TEMPORARY IMMERSION SYSTEM

Cortes¹

2017

Rev.Mex.Ing.Quim.

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G. Inei-Shizukawa, H. A. Velasco-Bedrán, G. F. Gutiérrez-López and H. Hernández-Sánchez Abstract Castilleja tenuiflora Benth, is a medicinal plant that synthesizes secondary metabolites such as phenylethanoid glycosides (PhGs), flavonoids, lignans, and iridoid glycosides with pharmacological properties. Nutrient deficiency stress in in vitro culturing of plants has been shown to be a good biotechnological strategy to stimulate secondary metabolism. Our aim was to evaluate morphogenesis (growth and development) and the production of secondary metabolites in C. tenuiflora shoots in response to stress by nitrogen (N) deficiency and starvation in temporary immersion bioreactors. Shoots were grown in B5 liquid medium with 25.74 mM (control), 1.32, 0.66 (deficiency) and 0 mM (starvation) N concentration. In all treatments, matter and shoot multiplication rate (SMR) decreased over 40%, chlorophylls content decreased, roots length increased, and leaves showed chlorosis respect to control. Under 0.66 mM N deficiency, the enzyme phenylalanine ammonia lyase (PAL) was more active (twice more than control) and total phenolic compounds were enhanced. At day 21: maximum concentration of PhGs (verbascoside and isoverbascoside) and iridoids (aucubin) was observed in 0.66, 1.32 and 0 mM N treatments (283 ± 0.5, 90 ± 0.3 and 12.5 ± 0.1 mg g −1 DM, respectively); these concentrations were 175, 143 and 34% greater than respective controls on the same day. Additionally, presence of anthocyanins was evident in stems of all plants developed under N deficiency and starvation. Despite the fact that, the matter, SMR and chlorophylls decreased because N stress deficiency, the production of secondary metabolites was enhanced, demonstrating that in vitro culture in temporary immersion bioreactors under suitable nutrients deficit such as N is an appropriate strategy to stimulate secondary metabolism in C. tenuiflora. Keywords: abiotic stress, Aucubin, nitrogen starvation, phenylethanoid glycosides, RITA® bioreactor. ResumenCastilleja tenuiflora Benth., es una planta medicinal que sintetiza metabolitos secundarios como feniletanoides glicosilados (PhGs), flavonoides, lignanos, e iridoides; que presentan actividad farmacológica. El estrés por deficiencia de nutrientes en el cultivo de plantas in vitro ha mostrado ser una estrategia biotecnológica apropiada para estimular su metabolismo secundario. En este trabajo se evaluaron la morfogénesis (crecimiento y desarrollo) y la producción de metabolitos secundarios en brotes de C. tenuiflora en biorreactores de inmersión temporal expuestos a estrés por deficiencia y ausencia de nitrógeno (N). El estrés se aplicó a un cultivo de los brotes cultivados en medio líquido B5 con 25.74 mM (control), 1.32, 0.66 (deficiencia) y 0 mM (ausencia) de N. En comparación con el control, todos los tratamientos mostraron una disminución de más del 40% en la biomasa y elíndice de multiplicación de brotes, el contenido de clorofila disminuyó, la longitud de las raíces aumentó y las hojas mostraron clorosis. En defi...

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Influence of repeated short-term nitrogen limitations on leaf phenolics metabolism in tomato

Cited by 66 publications

References 54 publications

Nitrogen and water availability to tomato plants triggers bottom-up effects on the leafminer Tuta absoluta

Nitrogen and water availability to tomato plants triggers bottom-up effects on the leafminer Tuta absoluta

How Exogenous Selenium Affects Anthocyanin Accumulation and Biosynthesis-Related Gene Expression in Purple Lettuce

MORPHOGENESIS AND SECONDARY METABOLITES PRODUCTION IN THE MEDICINAL PLANT Castilleja tenuiflora Benth. UNDER NITROGEN DEFICIENCY AND STARVATION STRESS IN A TEMPORARY IMMERSION SYSTEM

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