Influence of relative humidity in sensing halogenated hydrocarbons with Reflectometric Interference Spectroscopy (RIfS)

Kaspar, S.; Rathgeb, F.; Nopper, Dirk; Gauglitz, Günter

doi:10.1007/s002160051170

Cited by 7 publications

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“…[112][113][114][115] It is used for measurements in the gas phase, the detection of herbicides in water, for antigen-antibody binding assays, for separation of chiral substances, and even for detection of cell adhesion. [116][117][118][119][120] In our study, a silicon wafer with an oxide layer of 5 µm thickness is used as a substrate for vesicle spreading and covalent binding of molecules. The thickness of adsorbed layers with a refractive index of n i can be determined by detecting the reflectivity of the surface and by calculating optical thickness OT values while accounting for the Fresnel coefficients given by and the following relation: 43…”

Section: Principlementioning

confidence: 99%

A Force Spectroscopy Setup to Mimic Cellular Interaction Processes

Lorenz¹

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ment of a biophysical model system, which serves to quantify aspects of cell-virus (A), cell-cell (B), and cell-substrate (C) interactions.Moreover, controlled attachment and detachment regulate leukocyte binding to endothelium during inflammation processes. The key to cope with viral infections is to understand the reactions that take place at the first contact of cell and virus. Furthermore, the establishment of cell to substrate contacts is of crucial importance for the attachment of cells to connective tissue or the inclusion of rigid material like bone or cartilage into surrounding tissue. The Marfan syndrome for instance is characterized by a dysfunctionality of connective tissue resulting in aorta and skeleton malfunctions. [3] In these entire pathological processes vital cell signaling processes are influenced due to the lack or the occurrence of specific interactions between the cell and its periphery. Most relevant structures on cell surfaces are lectins, cadherins, and integrins as well as polysaccharide-bearing lipids.1 Schematic is not drawn to scale. All schematics are drawn by B. L. unless otherwise stated. rized by microvilli structures (A) enlarging the surface area available for the cells' uptake of nutrients. The lateral and basal membranes form borders to adjacent cells and the substrate, respectively. Tight junctions (B), adhesion junctions (C), desmosomes (D) and gap junctions (E) are functional units on the lateral membranes, whereas focal contacts (F) and hemidesmosomes (G) in the basal membrane are relevant for cellsubstrate interaction processes (H). Cytoskeletal components such as intermediated filaments (hemidesmosomes, desmosomes) and actin (adhesion junctions, focal contacts) are connected to these units. Gap junctions control the transport of small molecules, whereas tight junctions have a fence function. Tight junctions seal the intercellular space from the lumen of the tissue as they build barriers between the apical and the basolateral membranes of the cell. Gap junctions control the transport of small molecules between the cytosol of two adjacent cells, whereas the other structures shown on the lateral and the basal side of the cell act as mechanical transducers between the cells. Adherence junctions and focal contacts (basal cell membrane Figure 1.4-F) are coupled to actin filaments, whereas hemidesmosomes (basal membrane, Figure 1.4-G) 3 Image adapted from 6. 6. Wegener, J. (2001). Cell Junctions. eLS, John Wiley & Sons, Ltd.6 4 A list of abbreviations can be found in chapter 8.1 on page 126. 5 Image of Pietzsch, J., "The fluid-mosaic model of the cell membrane", nature.com, Nov 26, 2012, reprinted as a slightly modified version with permission of Nature Publishing Group. 6 Image is a snapshot taken from the video "The Inner Life of the Cell", Harvard Biovision, Leigh Stimolo, Alain Viel. glycero-3-phosphocholine (DPPC, B), 1,2-dioleyl-sn-glycero-3-phosphocholine (DOPC, C), or 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS, D) can be equipped with functionalized lipi...

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Section: Principlementioning

confidence: 99%

A Force Spectroscopy Setup to Mimic Cellular Interaction Processes

Lorenz¹

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Optical Gas Sensors in Analytical Chemistry: Applications, Trends and General Comments

Rathgeb

Gauglitz

2000

Encyclopedia of Analytical Chemistry

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Direct optical sensors: principles and selected applications

2004

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In the field of bio and chemosensors a large number of detection principles has been published within the last decade. These detection principles are based either on the observation of fluorescence-labelled systems or on direct optical detection in the heterogeneous phase. Direct optical detection can be measured by remission (absorption of reflected radiation, opt(r)odes), by measuring micro-refractivity, or measuring interference. In the last case either Mach-Zehnder interferometers or measurement of changes in the physical thickness of the layer (measuring micro-reflectivity) caused, e.g., by swelling effects in polymers (due to interaction with analytes) or in bioassays (due to affinity reactions) also play an important role. Here, an overview of methods of microrefractometric and microreflectometric principles is given and benefits and drawbacks of the various approaches are demonstrated using samples from the chemo and biosensor field. The quality of sensors does not just depend on transduction principles but on the total sensor system defined by this transduction, the sensitive layer, data acquisition electronics, and evaluation software. The intention of this article is, therefore, to demonstrate the essentials of the interaction of these parts within the system, and the focus is on optical sensing using planar transducers, because fibre optical sensors have been reviewed in this journal only recently. Lack of selectivity of chemosensors can be compensated either by the use of sensor arrays or by evaluating time-resolved measurements of analyte/sensitive layer interaction. In both cases chemometrics enables the quantification of analyte mixtures. These data-processing methods have also been successfully applied to antibody/antigen interactions even using cross-reactive antibodies. Because miniaturisation and parallelisation are essential approaches in recent years, some aspects and current trends, especially for bio-applications, will be discussed. Miniaturisation is especially well covered in the literature.

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Influence of relative humidity in sensing halogenated hydrocarbons with Reflectometric Interference Spectroscopy (RIfS)

Cited by 7 publications

References 0 publications

A Force Spectroscopy Setup to Mimic Cellular Interaction Processes

A Force Spectroscopy Setup to Mimic Cellular Interaction Processes

Optical Gas Sensors in Analytical Chemistry: Applications, Trends and General Comments

Direct optical sensors: principles and selected applications

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