Influence of regeneration and selection procedures on the production of aluminium tolerant potato regenerants

Wersuhn, G.; Gienapp, R.; Reinke, G.

doi:10.1007/bf02358356

Cited by 6 publications

(1 citation statement)

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“…This observation is consistent with those reported by Petolino and Collins (1985) who found that callus derived from Mn-tolerant clones of tobacco were more tolerant to Mn than non-tolerant ones. Purnhauser & Gyulai (1993) and Wersuhn et al (1994) reported that copper and aluminium tolerant plants were developed in wheat, triticale, tobacco and potato respectively through in vitro system. The regenerated tolerant plants rapidly multiplied on similar induction medium; the rate of growth, however, depended on growth regulators and heavy metal concentration as reported earlier in monocots (Purnhauser 1991 andRoustan et al 1989).…”

Section: Discussionmentioning

confidence: 99%

In vitro selection and characterization of Ni-tolerant callus lines of Setaria italica L

1998

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Nickel tolerant callus lines of Setaria italica L. were developed from callus cultures grown on MS medium supplemented with 3 3 3 +2 0.5 mg.dm-kinetin + 2.0 mg.dm 2,4-D + 2.0 mg.dm-Ni .Standard growth parameters such as callus fresh and dry weight, growth tolerance index were used as indicators of nickel toxicity. Measurements as early as 2 weeks after the beginning of the treatments did not yield consistent results. However, growth tolerance index at 4, and 8 weeks after the beginning of treatments yielded significant differences among the non-tolerant and tolerant calli. The tolerant calli has enhanced growth at 2.0 mg.dm -3 Ni +2 while non-tolerant calli showed a reverse trend in growth in the presence of 2.0-2.5 mg.dm -3 of nickel. The tolerant calli differentiated into mass of embryogenic calli within 4 weeks of culture which could be maintained for prolonged period without loss of regenerative capacity.

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Section: Discussionmentioning

confidence: 99%