Influence of recombinant duck follistatin protein on embryonic muscle development and gene expressions

Liu, H.; Zhang, R.; Li, X.; Sun, Lin; Wang, H.; Yang, Chao; Li, L.; Wang, J.; Xu, Feng

doi:10.1111/jpn.12104

Cited by 2 publications

(2 citation statements)

References 29 publications

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“…Previous studies in our laboratory showed that recombinant duck FST protein could promote muscle hypertrophy in the post-hatching duck by inducing satellite cell proliferation [ 8 ]. However, FST played minor roles in embryonic skeletal muscle development [ 9 ]. To date, the regulatory mechanism of FST in the skeletal muscle development of birds remains an open question.…”

Section: Introductionmentioning

confidence: 99%

Follistatin could promote the proliferation of duck primary myoblasts by activating PI3K/Akt/mTOR signalling

Liu

Wang

et al. 2014

Bioscience Reports

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FST (follistatin) is essential for skeletal muscle development, but the intracellular signalling networks that regulate FST-induced effects are not well defined. We sought to investigate whether FST promotes the proliferation of myoblasts through the PI3K (phosphoinositide 3-kinase)/Akt (protein kinase B)/mTOR (mammalian target of rapamycin) signalling. In the present study, we transfected the pEGFP-duFST plasmid and added PI3K and mTOR inhibitors to the medium of duck primary myoblasts. Then, we analysed the cellular phenotypic changes that occurred and analysed the expression of target genes. The results showed that FST promoted myoblast proliferation, induced the mRNA expression of PI3K, Akt, mTOR, 70-kDa ribosomal protein S6K (S6 kinase) and the protein expression of phospho-Akt (Thr308), mTOR, phospho-mTOR (serine 2448), phospho-S6K (Ser417), inhibited the mRNA expression of FoxO1, MuRF1 (muscle RING finger-1) and the protein expression of phospho-FoxO1 (Ser256). Moreover, we found that the overexpression of FST could alleviate the inhibitory effect of myoblast proliferation caused by the addition of LY294002, a PI3K inhibitor. Additionally, the overexpression of duck FST also relieved the inhibition of myoblast proliferation caused by the addition of rapamycin (an mTOR inhibitor) through PI3K/Akt/mTOR signalling. In light of the present results, we hypothesize that duck FST could promote myoblast proliferation, which is dependent on PI3K/Akt/mTOR signalling.

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Section: Introductionmentioning

confidence: 99%

Follistatin could promote the proliferation of duck primary myoblasts by activating PI3K/Akt/mTOR signalling

Liu

Wang

et al. 2014

Bioscience Reports

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“…Mainly, Pax7 mRNA expression was upregulated in breast and leg muscle, MyoD mRNA was increased only in leg muscle while Myf5 mRNA was upregulated only in breast muscle. Moreover, the expression of myostatin was downregulated in both muscle types ( 206 ). The effects of vitamin C on duck embryos' hatchability were investigated by Nowaczewski et al ( 207 ).…”

Section: In Ovo Effects In Other Avian Speciesmentioning

confidence: 99%

Physiological effects of in ovo delivery of bioactive substances in broiler chickens

Kpodo

Proszkowiec‐Weglarz²

2023

Front. Vet. Sci.

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The poultry industry has improved genetics, nutrition, and management practices, resulting in fast-growing chickens; however, disturbances during embryonic development may affect the entire production cycle and cause irreversible losses to broiler chicken producers. The most crucial time in the chicks' development appears to be the perinatal period, which encompasses the last few days of pre-hatch and the first few days of post-hatch. During this critical period, intestinal development occurs rapidly, and the chicks undergo a metabolic and physiological shift from the utilization of egg nutrients to exogenous feed. However, the nutrient reserve of the egg yolk may not be enough to sustain the late stage of embryonic development and provide energy for the hatching process. In addition, modern hatchery practices cause a delay in access to feed immediately post-hatch, and this can potentially affect the intestinal microbiome, health, development, and growth of the chickens. Development of the in ovo technology allowing for the delivery of bioactive substances into chicken embryos during their development represents a way to accommodate the perinatal period, late embryo development, and post-hatch growth. Many bioactive substances have been delivered through the in ovo technology, including carbohydrates, amino acids, hormones, prebiotics, probiotics and synbiotics, antibodies, immunostimulants, minerals, and microorganisms with a variety of physiological effects. In this review, we focused on the physiological effects of the in ovo delivery of these substances, including their effects on embryo development, gastrointestinal tract function and health, nutrient digestion, immune system development and function, bone development, overall growth performance, muscle development and meat quality, gastrointestinal tract microbiota development, heat stress response, pathogens exclusion, and birds metabolism, as well as transcriptome and proteome. We believe that this method is widely underestimated and underused by the poultry industry.

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Influence of recombinant duck follistatin protein on embryonic muscle development and gene expressions

Cited by 2 publications

References 29 publications

Follistatin could promote the proliferation of duck primary myoblasts by activating PI3K/Akt/mTOR signalling

Follistatin could promote the proliferation of duck primary myoblasts by activating PI3K/Akt/mTOR signalling

Physiological effects of in ovo delivery of bioactive substances in broiler chickens

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