Influence of reactor configuration on the production of carvone from carveol by whole cells of Rhodococcus erythropolis DCL14

Carvalho, Carla C. C. R. de; Fonseca, M. Manuela R. da

doi:10.1016/s1381-1177(02)00189-3

Cited by 29 publications

(12 citation statements)

References 10 publications

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“…Tables 1 and 2). Another reactor type is the airlift or bubble column reactor where interface mixing is accomplished by gas bubbles [27]. Bioconversion or de novo synthesis of isoprenoids in liquid-impelled loop reactors [98] have not yet been described for isoprenoid bioprocesses.…”

Section: Two-liquid Phase Bioprocessesmentioning

confidence: 99%

“…Other effects of the organic phase on biocatalyst or reaction can be: an influence on the enantiomeric specificity of the reaction accompanied by reduction of the e.e. value [96] or degradation of the organic phase by the microorganism [27].…”

Section: Two-liquid Phase Bioprocessesmentioning

confidence: 99%

“…Of course application of undiluted terpenoid substrate or product in an aqueous-organic two-phase system implies insignificant toxic and inhibitory effects on the biocatalyst. Biofilm formation has been described as disadvantageous in terpene bioconversion studies when they interfered with substrate or product transport between the extractive phase and the biocatalyst [27,57], however, biofilms can be used deliberately in biocatalytic approaches. Using catalytic biofilms allows for the exploitation of the advantages of a continuous reactor operation such as cell reuse, steady-state conditions with constant product qualities, and avoidance of downtimes, while at the same time the disadvantages of immobilization including reduced absolute activity and viability of the biocatalyst, additional costs, and lack 268 H. Schewe et al…”

Section: Two-liquid Phase Bioprocessesmentioning

confidence: 99%

“…The silicone membrane swelling must be considered in membrane bioreactor design to prevent membrane bursting or solvent breakthrough. Silicone rubber tubing submerged into a liquid phase of a stirred-tank reactor is a practical and straightforward implementation of a membrane reactor [14,27]. The organic phase may be pumped through the silicone rubber tubing which is submerged in the aqueous phase such as during the bioconversion of geraniol to citronellol by S. cerevisiae [29] or the bioconversion of α-pinene oxide to isonovalal by Pseudomonas fluorescens NCIMB 11671 [14].…”

Section: Perstraction In Membrane Reactorsmentioning

confidence: 99%

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Bioprocess Engineering for Microbial Synthesis and Conversion of Isoprenoids

Schewe

Mirata²,

Schrader

2015

Biotechnology of Isoprenoids

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Isoprenoids represent a natural product class essential to living organisms. Moreover, industrially relevant isoprenoid molecules cover a wide range of products such as pharmaceuticals, flavors and fragrances, or even biofuels. Their often complex structure makes chemical synthesis a difficult and expensive task and extraction from natural sources is typically low yielding. This has led to intense research for biotechnological production of isoprenoids by microbial de novo synthesis or biotransformation. Here, metabolic engineering, including synthetic biology approaches, is the key technology to develop efficient production strains in the first place. Bioprocess engineering, particularly in situ product removal (ISPR), is the second essential technology for the development of industrial-scale bioprocesses. A number of elaborate bioreactor and ISPR designs have been published to target the problems of isoprenoid synthesis and conversion, such as toxicity and product inhibition. However, despite the many exciting applications of isoprenoids, research on isoprenoid-specific bioprocesses has mostly been, and still is, limited to small-scale proof-of-concept approaches. This review presents and categorizes different ISPR solutions for biotechnological isoprenoid production and also addresses the main challenges en route towards industrial application.

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Section: Two-liquid Phase Bioprocessesmentioning

confidence: 99%

Section: Two-liquid Phase Bioprocessesmentioning

confidence: 99%

Section: Two-liquid Phase Bioprocessesmentioning

confidence: 99%

Section: Perstraction In Membrane Reactorsmentioning

confidence: 99%

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Bioprocess Engineering for Microbial Synthesis and Conversion of Isoprenoids

Schewe

Mirata²,

Schrader

2015

Biotechnology of Isoprenoids

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“…therapeutic steroids [1], biofuels [2] and terpenoids [3]. The combination of the natural diversity with further synthetic modifications provides an unlimited resource for complex compounds unmatched by chemical synthesis [4,5].…”

Section: Introductionmentioning

confidence: 99%

Anchoring high-throughput screening methods to scale-up bioproduction of siderophores

Marques

Walshe

Doyle

et al. 2012

Process Biochemistry

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a b s t r a c tThe use of high throughput strategies is of acknowledged relevance since the rational use of small-scale reactors, coupled with suitable analytic tools, is contributing to the acceleration of process development in several areas of biotechnology. These small-scale reactors are available in different working volumes and configurations, being useful in a wide array of applications, from cell screening to process optimization.The present work was focused on the development of a high-throughput strategy, combining microtiter plates and analytic methodologies, to screen an in-house library of environmental bacteria in order to identify good siderophore producers. From a library of roughly 500 marine microorganisms, it was possible to ultimately obtain 11 bacterial strains with high production capabilities. Two of them had not been previously identified as siderophore producers. The bioprocess was scaled-up from microtiter plates to a 5 L stirred tank reactor, while maintaining the overall volumetric productivity, using the k L a similarity as scale-up criterion.This novel approach is a suitable alternative to traditional screening tools.

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Oxidation of Alcohols, Aldehydes, and Acids

Hollmann

Bühler

2012

Enzyme Catalysis in Organic Synthesis

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Influence of reactor configuration on the production of carvone from carveol by whole cells of Rhodococcus erythropolis DCL14

Cited by 29 publications

References 10 publications

Bioprocess Engineering for Microbial Synthesis and Conversion of Isoprenoids

Bioprocess Engineering for Microbial Synthesis and Conversion of Isoprenoids

Anchoring high-throughput screening methods to scale-up bioproduction of siderophores

Oxidation of Alcohols, Aldehydes, and Acids

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