Influence of reactive oxygen species on the sterilization of microbes

Uhm, Han S.; Choi, Eun Ha; Cho, Guang S.; Hwang, Daniel

doi:10.1016/j.cap.2012.12.018

Cited by 11 publications

(9 citation statements)

References 14 publications

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“…A comprehensive understanding of the influence of reactive oxygen species on microbe sterilization was also presented by our group [ 223 ]. A sterilization experiment with bacterial endospores reveals that an argon-oxygen plasma jet very effectively kills endospores of Bacillus atrophaeus (ATCC 9372), thereby indicating that oxygen radicals are the key element of sterilization.…”

Section: Health and Hygiene Applications Of Nonthermal Biocompatible mentioning

confidence: 99%

Plasma bioscience and its application to medicine

Choi

Uhm²,

Kaushik

2021

AAPPS Bull.

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Nonthermal atmospheric pressure biocompatible plasma (NBP), alternatively called bio-cold plasma, is a partially ionized gas that consists of charged particles, neutral atoms and molecules, photons, an electric field, and heat. Recently, nonthermal plasma-based technology has been applied to bioscience, medicine, agriculture, food processing, and safety. Various plasma device configurations and electrode layouts has fast-tracked plasma applications in the treatment of biological and material surfaces. The NBP action mechanism may be related to the synergy of plasma constituents, such as ultraviolet radiation or a reactive species. Recently, plasma has been used in the inactivation of viruses and resistant microbes, such as fungal cells, bacteria, spores, and biofilms made by microbes. It has also been used to heal wounds, coagulate blood, degrade pollutants, functionalize material surfaces, kill cancers, and for dental applications. This review provides an outline of NBP devices and their applications in bioscience and medicine. We also discuss the role of plasma-activated liquids in biological applications, such as cancer treatments and agriculture. The individual adaptation of plasma to meet specific medical requirements necessitates real-time monitoring of both the plasma performance and the target that is treated and will provide a new paradigm of plasma-based therapeutic clinical systems.

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Section: Health and Hygiene Applications Of Nonthermal Biocompatible mentioning

confidence: 99%

Plasma bioscience and its application to medicine

Choi

Uhm²,

Kaushik

2021

AAPPS Bull.

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“…2c ) that EB or reactive oxygen species and hydroxyl free radicals of EB-generating might directly or indirectly rupture the cell membranes of C . destructans chlamydospores, hyphae, and conidia with a size greater than 10 μm, causing the release of the cytoplasm 43 , 44 .…”

Section: Resultsmentioning

confidence: 99%

Continuous cropping of endangered therapeutic plants via electron beam soil-treatment and neutron tomography

Sim

Seong²,

Kim³

et al. 2018

Sci Rep

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Various medicinal plants are threatened with extinction owing to their over-exploitation and the prevalence of soil borne pathogens. In this study, soils infected with root-rot pathogens, which prevent continuous-cropping, were treated with an electron beam. The level of soil-borne fungus was reduced to ≤0.01% by soil electron beam treatment without appreciable effects on the levels of antagonistic microorganism or on the physicochemical properties of the soil. The survival rate of 4-year-old plant was higher in electron beam-treated soil (81.0%) than in fumigated (62.5%), virgin (78%), or untreated-replanting soil (0%). Additionally, under various soils conditions, neutron tomography permitted the monitoring of plant health and the detection of root pathological changes over a period of 4–6 years by quantitatively measuring root water content in situ. These methods allow continual cropping on the same soil without pesticide treatment. This is a major step toward the environmentally friendly production of endangered therapeutic herbs.

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“…2A-2B) suggest that NO radicals, OH radicals, and the N 2 second-positive system (SPS) were significantly produced, but the generation of OH radicals is much more compared to NO radicals. The excited nitrogen molecules in a metastable level of N ) dissociate water molecules, and generate hydroxyl radicals and hydrogen atoms, which is one of the most important reactive species [6,19]. Plasma discharge in the nitrogen gas mixed with water molecules produces NH radicals, but the density of NH radicals is 5 orders in magnitude less than the hydroxyl density [39], indicating that hydroxyl in the nitrogen plasma dominates over other species except hydrogen peroxides.…”

Section: Discussionmentioning

confidence: 99%

“…The ability of OH radicals to induce biological damage is often exploited in the medical fields as therapeutic tools. Among these, reactive oxygen species (ROS) generated by non-thermal atmospheric-pressure plasma are an important means of inactivating microbial pathogens [5,6]. More recently, the efficiency of the cold argon plasma jet and dielectric barrier discharge to inactivate mycobacteria were reported [7,8].…”

Section: Introductionmentioning

confidence: 99%

Inactivation of Mycobacteria by Radicals from Non-Thermal Plasma Jet

Lee¹,

Subhadra²,

Choi³

et al. 2019

Journal of Microbiology and Biotechnology

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Mycobacterial cell walls comprise thick and diverse lipids and glycolipids that act as a permeability barrier to antibiotics or other chemical agents. The use of OH radicals from a non-thermal plasma jet (NTPJ) for the inactivation of mycobacteria in aqueous solution was adopted as a novel approach. Addition of water vapor in a nitrogen plasma jet generated OH radicals, which converted to hydrogen peroxide (H 2 O 2) that inactivated non-pathogenic Mycobacterium smegmatis and pathogenic Mycobacterium tuberculosis H37Rv. A stable plasma plume was obtained from a nitrogen plasma jet with 1.91 W of power, killing Escherichia coli and mycobacteria effectively, whereas addition of catalase decreased the effects of the former. Mycobacteria were more resistant than E. coli to NTPJ treatment. Plasma treatment enhanced intracellular ROS production and upregulation of genes related to ROS stress responses (thiolrelated oxidoreductases, such as SseA and DoxX, and ferric uptake regulator furA). Morphological changes of M. smegmatis and M. tuberculosis H37Rv were observed after 5 min treatment with N 2 +H 2 O plasma, but not of pre-incubated sample with catalase. This finding indicates that the bactericidal efficacy of NTPJ is related to the toxicity of OH and H 2 O 2 radicals in cells. Therefore, our study suggests that NTPJ treatment may effectively control pulmonary infections caused by M. tuberculosis and nontuberculous mycobacteria (NTM) such as M. avium or M. abscessus in water.

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Influence of reactive oxygen species on the sterilization of microbes

Cited by 11 publications

References 14 publications

Plasma bioscience and its application to medicine

Plasma bioscience and its application to medicine

Continuous cropping of endangered therapeutic plants via electron beam soil-treatment and neutron tomography

Inactivation of Mycobacteria by Radicals from Non-Thermal Plasma Jet

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