Influence of rat bone marrow mesenchymal stem cells to rat Langerhans islets viability during co-cultivation with microst ructured collagen‑containing hydrogel
Abstract:Aim. To research the rat bone marrow mesenchymal stem cells (MSC BM) effect to the viability of isolated rat OL during cultivation with microstructured collagen-containing hydrogel (BMCH).Materials and methods. Rat islets and MSC BM were isolated according to classical technique with some modifications. The experiments on the co- cultivation were carried out under standard conditions. Islet’s viability was determined by immunofluorescence staining.Results. TheOL cultivation with BMCH and MSCBM resulted in de… Show more
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